Neurodegeneration : methods and protocols

著者

    • Manfredi, Giovanni
    • Kawamata, Hibiki

書誌事項

Neurodegeneration : methods and protocols

edited by Giovanni Manfredi, Hibiki Kawamata

（Methods in molecular biology / John M. Walker, series editor, 793）（Springer protocols）

Humana Press, c2011

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

Neurodegenerative disorders, such as Alzheimer's and Parkinson's disease are a growing burden on our society. Understanding and treating these common diseases is one of the major current challenges for the biomedical field. In Neurodegeneration: Methods and Protocols, experts in the field tackle this challenge and present cutting-edge analytical and technological approaches to study the mechanisms underlying neurodegenerative processes. Organizes into five sections, chapters detail protocols for isolating and culturing cells from the nervous system, production and use of animal models, characterization of cell death, analytical tools to study disease mechanisms, and therapeutic approaches for neurodegeneration. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Neurodegeneration: Methods and Protocols serves as an ideal guide to both novice and professional researchers in the field of neurodegeneration.

目次

Part I. Neurodegeneration 1. Introduction to Neurodegenerative Diseases and Related Techniques Hibiki Kawamata and Giovanni Manfredi 2. In vivo and In vitro Determination of Cell Death Markers in Neurons Nicole Naniche, Daniela Sua, and Piera Pasinelli 3. Degenerating Processes Identified by Electron Microscopic Immunocytochemical Methods Teresa A. Milner, Elizabeth M. Waters, Danielle C. Robinson, and Joseph P. Pierce Part II. Neural Cell Culture Techniques 4. Isolation and Culture of Neurons and Astrocytes from the Mouse Brain Cortex Hyun-Jeong Kim and Jordi Magrane 5. Isolation and Culture of Postnatal Spinal Motoneurons Carol Milligan and David Gifondorwa 6. Converting Human Pluripotent Stem Cells to Neural Tissue and Neurons to Model Neurodegeneration Stuart M. Chambers, Yvonne Mica, Lorenz Studer, and Mark J. Tomishima 7. Neural Differentiation of Induced Pluripotent Stem Cells Mark Denham and Mirella Dottori Part III. Models of Neurodegeneration 8. Developing Yeast Models of Human Neurodegenerative Disorders Alejandro Ocampo and Antoni Barrientos 9. Modeling Dopamine Neuron Degeneration in C. elegans Michelle L. Tucci, Adam J. Harrington, Guy A. Caldwell, and Kim A. Caldwell 10. Role of the Proteasome in Fly Models of Neurodegeneration Chun-Hung Yeh, Marlon Jansen, and Thomas Schmidt-Glenewinkel 11. Modeling Neurodegenerative Diseases in Zebrafish Embryos Angela Laird and Wim Robberecht 12. Practical Considerations of Genetic Rodent Models for Neurodegenerative Diseases Kindiya Geghman and Chenjian Li 13. Modeling Focal Cerebral Ischemia In vivo Katherine Jackman, Alexander Kunz, and Costantino Iadecola 14. Pharmacological Models of Parkison's Disease in Rodents Peter Klivenyi and Laszlo Vecsei 15. Behavioral Phenotyping of Mouse Models of Neurodegeneration Magali Dumont Part IV. Neurodegenerative Disease Mechanisms - Proteinopathies 16. Characterization of Amyloid Deposits in Neurodegenerative Diseases Ruben Vidal and Bernardino Ghetti 17. Detection of Protein Aggregates in Neurodegenerative Diseases Han-xiang Deng, Eileen H. Bigio, and Teepu Siddique 18. Assessment of Proteasome Impairment and Accumulation/Aggregation of Ubiquitinated Proteins in Neuronal Cultures Natura Myeku, Maria Jose Metcalfe, Qian Huang, and Maria Figueiredo-Pereira Part V. Neurodegenerative Disease Mechanisms - Mitochondria 19. Measurements of Threshold of Mitochondrial Transition Pore Opening in Intact and Permeabilized Cells by Flash Photolysis of Caged Calcium Andrey Y. Abramov and Michael R. Duchen 20. Isolation and Functional Assessment of Mitochondria from Small Amounts of Mouse Brain Tissue Christos Chinopoulos, Steven F. Zhang, Bobby Thomas, Vadim Ten, and Anatoly A. Starkov 21. Monitoring Mitophagy in Neuronal Cell Cultures Jianhui Zhu, Ruben K. Dagda, and Charleen T. Chu Part VI. Neurodegenerative Disease Mechanisms - Biological functions 22. Analysis of Vesicular Trafficking in Primary Neurons by Live Imaging Davide Tampellini and Gunnar K. Gouras 23. Surface Trafficking of Sodium Channels in Cells and in Hippocampal Slices Doo Yeon Kim and Dora M. Kovacs 24. Imaging Presynaptic Exocytosis in Corticostriatal Slices Minerva Yue, David Sulzer, and Nigel Bamford 25. Microscopic Imaging of Intracellular Calcium in Live Cells using Lifetime based Ratiometric Measurements of Oregon Green BAPTA-1 Carli Lattarulo, Diana Thyssen, Kishore V. Kuchibholta, Bradley T. Hyman, and Brian J. Bacskai 26. Electrophysiological Characterization of Neuromuscular Synaptic Dysfunction in Mice Yoshie Sugiura and Weichun Lin 27. Determination of Neurotransmitter Levels in Models of Parkinson's Disease by HPLC-ECD Lichuan Yang and Flint M. Beal 28. Designing, Performing, and Interpreting a Microarray-based Gene Expression Study Giovanni Coppola Part VII. Therapeutic Approaches in Neurodegeneration 29. Adeno-associated Viral Gene Transfer for Neurodegenerative Diseases Peter F. Morgenstern, Roberta Marongiu, Sergei A. Musatov, and Michael G. Kaplitt 30. Specific Gene Silencing using RNAi in Cell Culture Chunxing Yang, Linghua Qiu, and Zuoshang Xu 31. Stem Cell Transplantation for Spinal Cord Neurodegeneration Angelo C. Lepore and Nicholas J. Maragakis 32. Evaluation of Histone Deacetylase Inhibitors as Therapeutics for Neurodegenerative Diseases Elisabetta Soragni, Chunping Xu, Andrew Cooper, Heather L. Plasterer, James R. Rusche, and Joel M. Gottesfeld

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