Glycosaminoglycans : chemistry and biology
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Bibliographic Information
Glycosaminoglycans : chemistry and biology
（Methods in molecular biology / John M. Walker, series editor, v. 1229）（Springer protocols）
Humana Press, c2015
- hbk.
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
Glycosaminoglycans: Chemistry and Biology emphasizes several areas of glycosaminoglycan research especially analysis and application of GAGs using a variety of approaches. Chapters focus on chemical and enzymatic synthesis of GAGs for therapeutic purposes; biophysical and biochemical methods for studying GAG-protein interactions; molecular approaches for modulating and defining GAG biosynthesis; informatics approaches for deciphering GAG code; computational approaches for establishing specific and/or non-specific interactions and genetic and biochemical tools for manipulating/visualizing glycosaminoglycan expression and studying their functions in a variety of model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Glycosaminoglycans: Chemistry and Biology serves as a valuable manual for cutting edge methodologies and practical tips to overcome any obstacles with experimentation pertaining to chemistry and biology of glycosaminoglycans.
Table of Contents
Part I: Structure
1. Automated Synthesis Of Chondroitin Sulfate Oligosaccharides
Chien-Fu Liang, Heung Sik Hahm, Peter H. Seeberger
2. Enzymatic Synthesis Of Heparan Sulfate And Heparin
April Joice, Karthik Raman, Caitlin Mencio, Maritza Quintero, Spencer Brown, Thao Kim Nu Nguyen And Balagurunathan Kuberan
3. Production Of Size-Defined Heparosan, Heparan Sulfate, and Heparin Oligosaccharides By Enzymatic Depolymerization
Spencer Brown And Balagurunathan Kuberan
4. Chemical Modification Of Heparin And Heparosan
Karthik Raman, Balagurunathan Kuberan, and Sailaja Arungundram
5. Synthesis Of Sulfur Isotope Labeled Sulfate Donor, 3'-Phosphoadenosine-5'-Phosphosulfate, For Studying Glycosaminoglycan Functions
Caitlin Mencio, Vimal Swarup, Marcus Soliai, and Balagurunathan Kuberan
6. Preparation Of Isotope Enriched Heparan Sulfate Precursors For Structural Biology Studies Xylophone V. Victor, Vy My Tran, Balagurunathan Kuberan, and Thao Kim Nu Nguyen
7. Synthesis Of Glycosaminoglycan Mimetics Through Sulfation Of Polyphenols
Rami A. Al-Horani, Rajesh Karuturi, Stephen Verespy III, and Umesh R. Desai
8. Synthesis Of Selective Inhibitors Of Heparan Sulfate And Chondroitin Sulfate Proteoglycan Biosynthesis
Caitlin Mencio, Dinesh R. Garud, Balagurunathan Kuberan, nd Mamoru Koketsu
9. Ascidian (Chordata-Tunicata) Glycosaminoglycans: Extraction, Purification, Biochemical and Spectroscopic Analysis
Mauro S. G. Pavao
10. Human Blood Glycosaminoglycans: Isolation and Analysis
Md.Ferdous Anower-E-Khuda, and Koji Kimata
11. Chromatographic Molecular Weight Measurements For Heparin, Its Fragments And Fractions, and Other Glycosaminoglycans
Barbara Mulloy and John Hogwood
12. Mass Spectrometric Methods For The Analysis Of Heparin And Heparan Sulfate
Miroslaw Lech, Ishan Capila, and Ganesh V. Kaundinya
13. Validated Capillary Electrophoretic Assays For Disaccharide Composition Analysis Of Galactosaminoglycans In Biologic Samples and Drugs/Neutraceuticals
Athanasia P. Asimakopoulou, Christina Malavaki, Nikolaos A. Afratis, Achilleas D. Theocharis, Fotini N. Lamari, and Nikos K. Karamanos
14. Fast Screening Of Glycosaminoglycan Disaccharides By Fluorophore Assisted Carbohydrate Electrophoresis (Face): Applications To Biologic Samples And Pharmaceutical Formulations
Evgenia Karousou, Athanasia Asimakopoulou, Vassiliki Zafeiropoulou, Manuela Viola, Luca Monti, Antonio Rossi, Alberto Passi, and Nikos Karamanos
15. Capillary Electrophoretic Analysis Of Isolated Sulfated Polysaccharides To Characterize Pharmaceutical Products
Zachary Shriver And Ram Sasisekharan
16. Methods For Measuring Exchangeable Protons In Glycosaminoglycans
Consuelo N. Beecher And Cynthia K. Larive
17. Heparan Sulfate Structure: Methods To Study N-Sulfation and NDST Action
Anders Dagalv, Anders Lundequist, Beata Filipek-Gorniok, Tabea Dierker, Inger Eriksson, and Lena Kjellen
18. Analysis Of Hyaluronan Synthase Activity
Davide Vigetti, Evgenia Karousou, Manuela Viola, and Alberto Passi
19. A Rapid, Non-Radioactive Assay For Measuring Heparan Sulfate C-5 Epimerase Activity Using Hydrogen/Deuterium Exchange-Mass Spectrometry
Ponnusamy Babu, Xylophone V. Victor, Karthik Raman, and Balagurunathan Kuberan
20. Aggrecan - Approaches To Study Biophysical And Biomechanical Properties
Hadi Tavakoli Nia, Christine Ortiz, and Alan Grodzinsky
21. Use Of Flow Cytometry For Characterisation And Fractionation Of Cell Populations Based On Their Expression Of Heparan Sulfate Epitopes
Rebecca J Holley, Raymond Aa Smith, Els Ma Van De Westerlo, Claire L Pickford, Catherine Lr Merry, and Toin H Van Kuppevelt
22. A Transgenic Approach To Live Imaging Of Heparan Sulfate Modification Patterns
Matthew Attreed and Hannes E. Bu Low
Part II: Functions
23. Informatics Tools To Advance The Biology Of Glycosaminoglycans and Proteoglycans
Lewis J. Frey
24. Designing 'High-Affinity, High-Specificity' Glycosaminoglycan Sequences Through Computerized Modeling. Nehru Viji Sankaranarayanan, Aurijit Sarkar, Umesh R. Desai, and Philip D. Mosier
25. Using Isothermal Titration Calorimetry To Determine Thermodynamic Parameters Of Protein-Glycosaminoglycan Interactions
Amit K. Dutta, Joerg Roesgen, and Krishna Rajarathnam
26. Characterizing Protein-Glycosaminoglycan Interactions Using Solution NMR Spectroscopy
Prem Raj B. Joseph, Krishna Mohan Poluri, Krishna Mohan Sepuru, and Krishna Rajarathnam
27. Glycosaminoglycan-Protein Interaction Studies Using Fluorescence Spectroscopy
Rio S. Boothello, Rami A. Al-Horani, And Umesh R. Desai
28. Studying Glycosaminoglycan-Protein Interactions Using Capillary Electrophoresis
Aiye Liang and Umesh R. Desai
29. Histochemical Analysis Of Heparan Sulfate 3-O-Sulfotransferase Expression In Mouse Brain
Tomio Yabe And Nobuaki Maeda
30. Keratan Sulfate: Biosynthesis, Structures And Biological Functions
Kenji Uchimura
31. The Sulfs: Expression, Purification And Substrate Specificity
Kenji Uchimura
32. The Detection Of Glycosaminoglycans In Pancreatic Islets And Lymphoid Tissues
Marika Bogdani,Charmaine Simeonovic, Nadine Nagy, Pamela Y. Johnson, Christina K. Chan, and Thomas N. Wight
33. Non-Radioactive Glycosyltransferase And Sulfotransferase Assay To Study Glycosaminoglycan Biosynthesis
Cheryl M Ethen, Miranda Machacek, Brittany Prather, Timothy Tatge, Haixiao Yu, and Zhengliang L Wu
34. Mapping Proteoglycan Functions With Glycosidases
Mauricio Cortes, Leslie K. Cortes, and Nancy B. Schwartz
35. Cell Substrate Patterning With Glycosaminoglycans To Study Their Biological Roles In The Central Nervous System. Tony W. Hsiao, Vimal P. Swarup, Colin D. Eichinger, and Vladimir Hlady
36. Analyzing The Role Of Heparan Sulfate Proteoglycans In Axon Guidance In Vivo In Zebrafish
Fabienne E. Poulain
37. Murine Models In The Evaluation Of Heparan Sulfate-Based Anticoagulants
David Gailani, Qiufang Cheng, and Ivan S. Ivanov
38. Genetic Approaches In The Study Of Heparan Sulfate Functions In Drosophila
Masahiko Takemura and Hiroshi Nakato
39. Measuring Sulfatase Expression And Invasion In Glioblastoma
Anna Wade, Jane R. Engler, Vy My Tran, and Joanna J. Phillips
40. Synthesis And Biomedical Applications Of Xylosides
Mausam Kalita, Maritza V. Quintero, Karthik Raman, Vy M. Tran, and Balagurunathan Kuberan
41. A Strategic Approach To Identification Of Selective Inhibitors Of Cancer Stem Cells
Nirmita Patel, Somesh Baranwal, and Bhaumik B. Patel
42. Analysis Of The Heavy Chain Modification And Tsg-6 Activity In Pathological Hyaluronan Matrices Mark E. Lauer, Jacqueline Loftis, Carol De La Motte, and Vincent C. Hascall
43. Heparan Sulfate Modulates Slit3-Induced Endothelial Cell Migration
Hong Qiu, Wen-Yuan Xiao, Jingwen Yue, and Lianchun Wang
44. Glycosaminoglycan Functionalized Nanoparticles Exploit Glycosaminoglycan Functions
James A. Vassie, John M. Whitelock, and Megan S. Lord
45. Role Of Glycosaminoglycans In Infectious Disease
Akiko Ohno-Jinno and Pyong Woo Park
46. Isolation And Purification Of Versican And Analysis Of Versican Proteolysis
Simon J. Foulcer , Anthony J. Day, and Suneel S. Apte
47. Analysis Of Human Hyaluronan Synthase Gene Transcriptional Regulation and Downstream Hyaluronan Cell Surface Receptor Mobility In Myofibroblast Differentiation
Adam C. Midgley and Timothy Bowen
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