Skeletal development and repair : methods and protocols

著者

    • Hilton, Matthew J.

書誌事項

Skeletal development and repair : methods and protocols

edited by Matthew J. Hilton

（Methods in molecular biology / John M. Walker, series editor, 1130）（Springer protocols）

Humana Press, c2014

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注記

Humana Press is a brand of Springer

Includes bibliographical references and index

内容説明・目次

内容説明

Skeletal Development and Repair: Methods and Protocols is a compilation of a variety of skeletal research protocols utilizing the laboratory mouse as the platform for surgical manipulation and/or transplantation as well as the source of tissues and cells for in vitro culture and analyses. Chapters are written by experts in the field and cover topics including surgical, transplantation and organ culture methods that permit analyses of skeletal tissues undergoing repair in vivo and permits analyses of cellular interactions ex vivo, histological and molecular techniques developed to study gene and protein expression in whole embryos, skeletal tissues and tissue sections and in vitro primary cell culture protocols designed to assay gene function in specific cell populations. Written in the successful Methods in Molecular Biology series format chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Skeletal Development and Repair: Methods and Protocols is a comprehensive laboratory manual for all levels of basic research scientists working in the broad fields of skeletal development and skeletal repair research.

目次

Part I. Understanding Mouse Skeletal Development and Repair 1. Overview of Skeletal Development Tatsuya Kobayashi and Henry M. Kronenberg 2. Overview of Skeletal Repair (Fracture Healing and Its Assessment) Elise F. Morgan, Anthony De Giacomo, and Louis C. Gerstenfeld Part II. Skeletal Repair, Transplantations, and Organ Cultures 3. Generation of Closed Transverse Fractures in Small Animals Anthony De Giacomo, Elise F. Morgan, and Louis C. Gerstenfeld 4. The Murine Femoral Bone Graft Model and a Semi-Automated Histomorphometric Analysis Tool Robinder S. Dhillon, Longze Zhang, Edward M. Schwarz, Brendan F. Boyce, and Chao Xie 5. Surgical Induction of Posttraumatic Osteoarthirits Daisuke Hamada, Erik R. Sampson, Robert D. Maynard, and Michael J. Zuscik 6. A Mouse Model of Flexor Tendon Repair Sys Hasslund, Regis J. O'Keefe, and Hani A. Awad 7. Cartilage Explant Cultures Manuela Wuelling and Andrew Vortkamp 8. Renal Capsule Transplantations to Assay Skeletal Angiogenesis Rana Abou-Khalil and Celine Colnot Part III. Histological and Molecular Analyses on Whole Tissues and Tissue Sections 9. Whole Mount Skeletal Staining Diana Rigueur and Karen M. Lyons 10. Tips and Techniques for Processing and Sectioning Undecalcified Murine Bone Specimens Thomas B. Bemenderfer, Jonathan S. Harris, Keith W. Condon, and Melissa A. Kacena 11. Preparation of Thin Sections from Non-fixed and Undecalcified Hard Tissues Using Kawamoto's Film Method (2012) Tadafumi Kawamoto and Komei Kawamoto 12. Demineralized Murine Skeletal Histology Sarah A. Mack, Kathleen M. Maltby, and Matthew J. Hilton 13. Beta-Galactosidase Staining in the Skeleton X.L. Han and Jian Q. Feng 14. Whole-Mount In Situ Hybridization on Murine Skeletogenic Tissues Timothy Rutkowsky, Deepika Sharma, and Matthew J. Hilton 15. Non-Radioactive In Situ Hybridization on Skeletal Tissue Sections Yulia Shwartz and Elazar Zelzer 16. Radioactive In Situ Hybridization to Detect Gene Expression in Skeletal Tissue Sections Kyu Sang Joeng, Jenna Regan, and Fanxin Long 17. Proliferation Assays (BrdU and EdU) on Skeletal Tissue Sections Timothy J. Mead and Veronique Lefebvre 18. TUNEL Assay on Skeletal Tissue Sections to Detect Apoptosis Laura Mangiavini and Ernestina Schipani Part IV. Primary Cell Isolations, Cultures, and Assays 19. Analysis of Chondrogenesis Using Micromass Cultures of Limb Mesenchyme T. Michael Underhill, Helen J. Dranse, and Lisa M. Hoffman 20. Isolation and Culture of Murine Primary Chondrocytes Anthony J. Mirando, Yufeng Dong, Jinsil Kim, and Matthew J. Hilton 21. Bone Marrow Stromal Cell Assays - In Vitro and In Vivo Pamela Gehron Rober, Sergei A. Kuznetsov, Mara Riminucci, and Paolo Bianco 22. Isolation and Culture of Neonatal Mouse Calvarial Osteoblasts Jennifer H. Jonason and Regis J. O'Keefe 23. RANKL-Based Osteoclastogenic Assays from Murine Bone Marrow Cells Lianping Xing and Brendan F. Boyce 24. Hematopoietic Stem Cell Cultures and Assays Benjamin J. Frisch and Laura M. Calvi

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