The BAM complex : methods and protocols

著者

    • Buchanan, Susan K.
    • Noinaj, Nicholas

書誌事項

The BAM complex : methods and protocols

edited by Susan Buchanan, Nicholas Noinaj

（Methods in molecular biology / John M. Walker, series editor, 1329）（Springer protocols）

Humana Press, c2015

  • : hardback

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers through methods on the function of the BAM complex, the roles played by each of the individual components, the expression and purification of the components, crystallization and structure determination of the components, and how the individual Bam components may assemble into a functional complex. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The BAM Complex: Methods and Protocols will serve as an invaluable reference for those interested in studying the BAM complex.

目次

1. The -barrel Assembly Machinery Complex Denisse L. Leyton, Matthew J. Belousoff, and Trevor Lithgow 2. Yeast Mitochondria as a Model System to Study the Biogenesis of Bacterial -barrel Proteins Thomas Ulrich, Philipp Oberhettinger, Ingo B. Autenrieth, and Doron Rapaport 3. Experimental Methods for Studying the BAM Complex in Neisseria meningitidis Martine P. Bos, Ria Tommassen-van Boxtel, and Jan Tommassen 4. Heat Modifiability of Outer Membrane Proteins from Gram-Negative Bacteria Nicholas Noinaj, Adam J. Kuszak, and Susan K. Buchanan 5. The Role of a Destabilized Membrane for OMP Insertion Ashlee M. Plummer, Dennis Gessmann, and Karen G. Fleming 6. Treponema pallidum in Gel Microdroplets: A Method for Topological Analysis of BamA (TP0326) and Localization of Rare Outer Membrane Proteins Amit Luthra, Arvind Anand, and Justin D. Radolf 7. Analyzing the Role of Periplasmic Folding Factors in the Biogenesis of OMPs and Members of the Type V Secretion System Gustavo Bodelon, Elvira Marin, and Luis Angel Fernandez 8. An In Vitro Assay for Substrate Translocation by FhaC in Liposomes Enguo Fan, Derrick Norell, and Matthias Muller 9. Measuring Cell-cell Binding Using Flow-Cytometry Zachary C. Ruhe, Christopher S. Hayes, and David A. Low 10. Methods to Characterize Folding and Function of BamA Crosslink Mutants Adam J. Kuszak, Nicholas Noinaj, and Susan K. Buchanan 11. Small Angle X-ray Scattering (SAXS) Characterization of the POTRA Domains of BamA Pamela Arden Doerner and Marcelo Carlos Sousa 12. Assessing the Outer Membrane Insertion and Folding of Multimeric Transmembrane -barrel Proteins Jack C. Leo, Philipp Oberhettinger, and Dirk Linke 13. The Expression, Purification, and Structure Determination of BamA from E. coli Dongchun Ni and Yihua Huang 14. Expression and Purification of the Individual Bam Components BamB-E Suraaj Aulakh, Kelly H. Kim, and Mark Paetzel 15. Structure Determination of the BAM Complex Accessory Lipoproteins BamB-E Kornelius Zeth 16. An In Vitro Assay for Outer Membrane Protein Assembly by the BAM complex Giselle Roman-Hernandez and Harris D. Bernstein 17. Identification of BamC on the Surface of E. coli Chaille T. Webb and Trevor Lithgow 18. Construction and Characterization of an E. coli bamD Depletion Strain Dante P. Ricci 19. Expression, Purification and Screening of BamE, a Component of the BAM Complex, for Structural Characterization Mark Jeeves, Pooja Sridhar, and Timothy J. Knowles 20. Purification and Bicelle Crystallization for Structure Determination of the E. coli Outer Membrane Protein TamA Fabian Gruss, Sebastian Hiller, and Timm Maier 21. Strategies for the Analysis of Bam Recognition Motifs in Outer Membrane Proteins Nagarajan Paramasivam and Dirk Linke 22. Summary and Future Directions Nicholas Noinaj and Susan K. Buchanan

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