Correlation force spectroscopy for single molecule measurements
著者
書誌事項
Correlation force spectroscopy for single molecule measurements
（Springer theses : recognizing outstanding Ph. D. research）
Springer, c2015
- : hardcover
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注記
Includes bibliographical references
内容説明・目次
内容説明
This thesis addresses the development of a new force spectroscopy tool, correlation force spectroscopy (CFS) for the measurement of the properties of very small volumes of material (molecular to m3) at kHz-MHz frequency range. CFS measures the simultaneous thermal fluctuations of two closely-spaced atomic force microscopy (AFM) cantilevers. CFS then calculates the cross-correlation in the thermal fluctuations that gives the mechanical properties of the matter that spans the gap of the two cantilevers. The book also discusses development of CFS, its advantages over AFM, and its application in single molecule force spectroscopy and micro-rheology.
目次
Chapter 1 - Introduction
Overview of Single Molecule Force Spectroscopy Techniques
Experiments Using Single molecule AFM
Limitations of AFM-based single molecule force spectroscopy
Summary
Chapter 2- Correlation Force Spectroscopy
Correlation Force Spectroscopy: Rationale
Correlation Force Spectroscopy: Development
Laterally Offset Configuration
Vertically Offset Configuration
Analysis of Correlations between Two Cantilevers
Validation of Fluctuation-Dissipation Theorem for One Cantilever
Analysis of Thermal Fluctuations to obtain Correlations
Chapter 3- Dynamics of Single Molecules
Elastic Properties of Single Molecules: Worm-Like Chain and Freely-Jointed Chain Models
Hydrodynamics of Single Molecules: Dumbbell Model and Rouse Model
Internal Friction
Rouse with Internal Friction
Model of Linear Viscoelasticity of a Semiflexible Chain
Summary
Chapter 4- Microrheology with Correlation Force Spectroscopy
Existing Techniques of Rheometry
Experimental Methods
Comparison to Finite Element Analysis
Comparison to Simple Harmonic Oscillator Model
Summary
Chapter 5- Development of Colloidal Probe Correlation Force Spectroscopy: Case Study
Materials and Methods
Analysis
Results
Discussion
Summary
Chapter 6- Correlation Force Spectroscopy for Single Molecule Measurements
Effect of the Distance between Cantilever Tips
Harmonic Oscillator Modeling of Vertically Offset Correlation Force Spectroscopy
Summary
Chapter 7- Single Molecule Force Spectroscopy of Dextran
Materials and Methods
Results
Stretching a Dextran Molecule
Static Force-Elongation Mode
Dynamic Correlated Fluctuations Mode
Discussion
Summary
Chapter 8- Single Molecule Force Spectroscopy of Single-Stranded DNA
Rationale
Introduction Materials and Methods
Modal Analysis
Results
Discussion
Summary
Chapter 9- Summary
An Overview of Chapters
Future Work
Appendix I: Simple Harmonic Oscillator Model for Laterally Offset Correlation Force Spectroscopy
Appendix II: Simple Harmonic Oscillator Model for Vertically Offset Correlation Force Spectroscopy with Tethered Molecule
References
「Nielsen BookData」 より