Gene therapy of solid cancers : methods and protocols

Author(s)

• Walther, Wolfgang
• Stein, Ulrike

Bibliographic Information

Gene therapy of solid cancers : methods and protocols

edited by Wolfgang Walther and Ulrike Stein

（Methods in molecular biology / John M. Walker, series editor, 1317）（Springer protocols）

Humana Press, c2015

Note

Includes bibliographical references and index

Description and Table of Contents

Description

This volume provides insight into recent developments on experimental and clinical strategies for cancer gene therapy. Gene Therapy of Solid Cancers: Methods and Protocols guides readers through protocols on gene therapeutic strategies in combination with helpful technical notes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, Gene Therapy of Solid Cancers: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Table of Contents

Part I: Immunogene Therapy 1. Aptamer Targeting the ERBB2 Receptor Tyrosine Kinase for Applications in Tumor Therapy Georg Mahlknecht, Michael Sela, and Yosef Yarden 2. Gene Gun Her2/neu DNA Vaccination: Evaluation Of Vaccine Efficacy in a Syngeneic Her2/neu Mouse Tumor Model Tam Nguyen-Hoai, Antonio Pezzutto, and Joerg Westermann 3. MIDGE Technology for the Production of a Fourfold Gene-Modified, Allogenic Cell-Based Vaccine For Cancer Therapy Manuel Schmidt, Barbara Volz, Patrick Grossmann, Kerstin Heinrich, and Burghardt Wittig Part II: Suicide Gene Therapy 4. Evaluation of Bystander Cell Killing Effects in Suicide Gene Therapy of Cancer: Engineered Thymidylate Kinase (TMPK)/AZT Enzyme-Prodrug Axis Takeya Sato, Anton Neschadim, Ryo Nakagawa, Teruyuki Yanagisawa, and Jeffrey A. Medin 5. Oncoleaking: Use of the Pore-forming Clostridium perfringens Enterotoxin (CPE) for Suicide Gene Therapy Jessica Pahle, Jutta Aumann, Dennis Kobelt, and Wolfgang Walther 6. iCaspase 9 Suicide Gene System Xiaoou Zhou, Antonio Di Stasi, and Malcolm K. Brenner Part III: Gene Replacement Therapies 7. p53-encoding pDNA Purification by Affinity-Chromatography for Cancer Therapy Angela Sousa, Joao A. Queiroz, and Fani Sousa 8. A qRT-PCR Method for Determining the Biodistribution Profile of a miR-34a Mimic Kevin Kelnar and Andreas G. Bader Part IV: Gene Suppression and Signaling Modulation Therapy 9. Design and Selection of Antisense Oligonucleotides Targeting Transforming Growth Factor Beta (TGF-b) Isoform mRNAs for the Treatment of Solid Tumors Frank Jaschinski, Hanna Korhonen, and Michel Janicot 10. RNA Interference for Antimetastatic Therapy Mathias Dahlmann and Ulrike Stein 11. STAT3 Decoy ODN Therapy for Cancer Xiaoxia Sun and Jian Zhang Part V: Oncolytic Virotherapy and Bacterial Vectors for Cancer Treatment 12. Oncolytic Viral Therapy using Reovirus Chandini Thirukkumaran and Don G. Morris 13. Use of GLV-1h68 for Vaccinia Virotherapy and Monitoring Alexa Frentzen, Ulrike Geissinger, Desislava Tsoneva, and Jochen Stritzker 14. Back To The Future: Are Tumor-Targeting Bacteria The Next Generation Cancer Therapy? Robert M. Hoffman Part IV: Clinical Application of Cancer Gene Therapy 15. Ethics of Cancer Gene Transfer Clinical Research Jonathan Kimmelman 16. Planning an Academic Clinical Trial Kim M. Champion and Gemma R. Jones 17. Production of Plasmid DNA as PharmaceuticalMarco Schmeer and Martin Schleef 18. Minicircle - Next Generation DNA Vectors for Vaccination Schleef, M., Schirmbeck, R., Michael Reiser2, M.-L. Michel, and Schmeer, M. VII: Protocols of Clinical Cancer Gene Therapy 19. A Phase 2, Open-Label, Randomized Study of Pexa-Vec (JX-594) Administered by Intratumoral Injection in Patients with Unresectable Primary Hepatocellular Carcinoma Caroline J. Breitbach, Anne Moon, James Burke, Tae-Ho Hwang, and David H. Kirn 20. Antiangiogenic Metargidin Peptide (AMEP) Gene Therapy in Disseminated MelanomaIben Spanggaard and Julie Gehl 21. Clinical Evaluation of ErbB-targeted CAR T-cells, Following Intra-Cavity Delivery in Patients with ErbB-Expressing Solid TumorsSophie Papa, May van Schalkwyk, and John Maher

by "Nielsen BookData"

Details

• NCID: BB20347726
• ISBN:
  • 9781493927265
• LCCN: 2015940999
• Country Code: us
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: New York
• Pages/Volumes: xiii, 387 p.
• Size: 27 cm
• Subject Headings:
  • LCSH : Cancer -- Gene therapy -- Laboratory manuals
• Parent Bibliography ID:
  • BA10794673
  • BA88873632