Fungal secondary metabolism : methods and protocols
Author(s)
Bibliographic Information
Fungal secondary metabolism : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 944）（Springer protocols）
Humana Press, 2012
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Includes bibliographical references and index
Description and Table of Contents
Description
Filamentous fungi have long been known for their ability to produce an enormous range of unusual chemical compounds known as secondary metabolites, many of which have potentially useful antibiotic or pharmacological properties. Recent focus on fungal genomics coupled with advances in detection and molecular manipulation techniques has galvanized a revitalization of this field. Fungal Secondary Metabolism: Methods and Protocols is aimed at providing the key methodologies currently in use and necessary for accessing and exploiting the natural product information provided by the genomes of this large and varied kingdom. Written by active researchers in the field, the chapters deal with all the steps necessary, from optimization of fungal culture conditions for metabolite production, through rapid genome sequencing and bioinformatics, and genetic manipulations for functional analysis, to detection and testing of metabolites. In addition, chapters on basic science address approaches to the genetic regulation, protein biochemistry, and cellular localization of the biosynthetic pathways. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and hands-on, Fungal Secondary Metabolism: Methods and Protocols encourages new investigators to enter the field and expands upon the expertise and range of skills of those already researching fungal natural products.
Table of Contents
1. Library Preparation and Data Analysis Packages for Rapid Genome Sequencing
Kyle R. Pomraning, Kristina M. Smith, Erin L. Bredeweg, Lanelle R. Connolly, Pallavi A. Phatale, and Michael Freitag
2. Bioinformatics Approaches and Software for Detection of Secondary Metabolic Gene Clusters
Natalie D. Fedorova, Venkatesh Moktali, and Marnix H. Medema
3. Media and Growth Conditions for Induction of Secondary Metabolite Production
Jens C. Frisvad
4. Manipulating Filamentous Fungus Chemical Phenotypes by Growth on Nutritional Arrays
Jose R. Tormo, Francisco J. Asensio, and Gerald F. Bills
5. The "FERMEX" Method for Metabolite-Enriched Fungal Extracts
Gerald F. Bills, Anne W. Dombrowski, and Michael A. Goetz
6. The Chemical Identification and Analysis of Aspergillus nidulans Secondary Metabolites
James F. Sanchez and Clay C.C. Wang
7. Detection and Purification of Non-Ribosomal Peptide Synthetase Products in Neosartorya fischeri
Beate Wollinsky and Shu-Ming Li
8. Production, Detection, and Purification of Clavine-Type Ergot Alkaloids
Christiane Wallwey and Shu-Ming Li
9. Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS)
Ludmila V. Roze, Randolph M. Beaudry, and John E. Linz
10. Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans
C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley
11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis
Jin Woo Bok and Nancy P. Keller
12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host
Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii
13. Multi-Copy Genetic Screen in Aspergillus nidulans
Hee Soo Park and Jae-Hyuk Yu
14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification
OEzgur Bayram, OEzlem Sarikaya Bayram, Oliver Valerius, Bastian Joehnk, and Gerhard H. Braus
15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra
Ry R. Forseth and Frank C. Schroeder
16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi
Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss
17. Targeted Proteomics for Metabolic Pathway Optimization
Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold
18. Investigation of In Vivo Protein Interactions in Aspergillus Spores
Kwang Cheol Jeong and Jae-Hyuk Yu
19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus
Anindya Chanda, Ludmila V. Roze, and John E. Linz
20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi
Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson
21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects
Ulrike Fohgrub and Frank Kempken
Jose R. Tormo, Francisco J. Asensio, and Gerald F. Bills
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