Isotope labeling of biomolecules : applications

著者

• Kelman, Zvi

書誌事項

Isotope labeling of biomolecules : applications

edited by Zvi Kelman

（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, 566）

Academic Press, c2016

• hbk.
• PDF ebook

注記

Includes bibliographical references and index

内容説明・目次

内容説明

Isotope Labeling of Biomolecules: Applications, the latest in the Methods in Enzymology series, focuses on stable isotope labeling methods and applications for biomolecules. This practical guide to biomolecular labeling looks at new techniques that are becoming widely used.

目次

Nuclear Magnetic Resonance Spectroscopy 1. Application of Natural Isotopic Abundance 1H-13C and 1H-15N Correlated Two- Dimensional NMR for Evaluation of the Structure of Protein Therapeutics Luke W. Arbogast, Robert G. Brinson and John P. Marino 2. A Semi-Automated Assignment Protocol for Methyl Group Side-Chains in Large Proteins Jonggul Kim, Yingjie Wang, Geoffrey Li and Gianluigi Veglia 3. 19F-site Specific Labeled Nucleotides for Nucleic Acid Structural Analysis by NMR Lincoln G. Scott and Mirko Hennig 4. Applying Thymine Isostere 2,4-Difluoro-5-methylbenzene as a NMR Assignment Tool and Probe of Homopyrimidine/Homopurine Tract Structural Dynamics Robert G. Brinson, Jennifer T. Miller, Jason D. Kahn, Stuart F. J. Le Grice and John P. Marino Scattering Techniques 5. Biomolecular Deuteration for Neutron Structural Biology and Dynamics Michael Haertlein, Martine Moulin, Juliette M. Devos, Valerie Laux, Orla Dunne, V. Trevor Forsyth 6. Deuterium Labeling Together with Contrast Variation Small-angle Neutron Scattering Suggests How Skp Captures and Releases Unfolded Outer Membrane Proteins Nathan R. Zaccai, Clifford W. Sandlin, James T. Hoopes, Joseph E. Curtis, Patrick J. Fleming, Karen G. Fleming and Susan Krueger 7. Deuteration in Biological Neutron Reflectometry Frank Heinrich 8. Deuterium Labelling Strategies for Creating Contrast in Structure-Function Studies of Model Bacterial Outer Membranes Using Neutron Reflectometry Anton P. Le Brun, Luke A. Clifton, Stephen A. Holt, Peter J. Holden, and Jeremy H. Lakey 9. Essential Strategies for Revealing Nanoscale Protein Dynamics By Neutron Spin Echo Spectroscopy David J. E. Callaway and Zimei Bu Mass Spectrometry 10. Method for the Determination of 15N Incorporation Percentage in Labeled Peptides and Proteins Eric L. Kilpatrick 11. QconCAT: Internal Standard for Protein Quantification Kerry Bauer Scott, Illarion V. Turko and Karen W. Phinney 12. Production, Purification and Characterization of 15N-Labeled DNA Repair Proteins as Internal Standards for Mass Spectrometric Measurements Prasad T. Reddy, Pawel Jaruga, Bryant C. Nelson, Mark S. Lowenthal, Ann-Sofie Jemth, Olga Loseva, Erdem Coskun, Thomas Helleday and Miral Dizdaroglu Hydrogen-Deuterium Exchange Mass Spectrometry 13. Hydrogen Exchange Mass Spectrometry Leland Mayne 14. Mapping Protein-Ligand Interactions with Proteolytic Fragmentation, Hydrogen/Deuterium Exchange-Mass Spectrometry Elyssia S. Gallagher and Jeffrey W. Hudgens 15. Isotope Labeling of Biomolecules "Structural analysis of viruses by HDX-MS" Miklos Guttman and Kelly K. Lee

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詳細情報

• NII書誌ID(NCID): BB2055498X
• ISBN:
  • 9780128030493
• 出版国コード: ne
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Amsterdam
• ページ数/冊数: xvi, 465 p., [30] p. of plates
• 大きさ: 24 cm
• 分類:
  • DC23 : 572.8
• 件名:
  • LCSH : Biophysical labeling
  • LCSH : Biomolecules
  • LCSH : Isotopes
• 親書誌ID:
  • BA00197543