Plant gravitropism : methods and protocols
Author(s)
Bibliographic Information
Plant gravitropism : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1309）（Springer protocols）
Humana Press, c2015
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
This volume provides the plant scientific community with a collection of established and recently developed experimental protocols to study plant gravitropism. The first few chapters in this book discuss topics such as methods to properly orient plant material for gravitropism studies; protocols for data collection and image analysis; and techniques to investigate ion, organelle, and auxin transporter dynamics, particularly in living cells, as the plant is responding to a change in its orientation. The next few chapters talk about topics that are essential for understanding the complexities underlying tropisms and plant movements in general, and outline basic protocols on handling ornamental flowering shoots for basic plant gravitropism studies. The book concludes with chapters that discuss plant biological studies in space in order to take advantage of unique microgravity conditions not available in Earth-based studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Informative and cutting-edge, Plant Gravitropism is the perfect book for researchers in the plant scientific community because it is not only useful for plant gravitropism studies, but also addresses a range of interesting problems in plant growth and development.
Table of Contents
1. Immunohistochemistry Relative to Gravity: A Simple Method to Retain Information About Gravity for Immunolocalization and Histochemistry
Benjamin R. Harrison and Patrick H. Masson
2. A Flat Embedding Method to Orient Thin Biological Samples for Sectioning
Utku Avci and Jin Nakashima
3. Quantification of Root Gravitropic Response Using a Constant Stimulus Feedback System
Chris Wolverton
4. The Analysis of Gravitropic Setpoint Angle Control in Arabidopsis
Suruchi Roychoudhry and Stefan Kepinski
5. Imaging of Dynamic Ion Signaling During Root Gravitropism
Gabriele B. Monshausen
6. Live Cell Imaging of Cytoskeletal and Organelle Dynamics in Gravity-Sensing Cells in Plant Gravitropism
Moritaka Nakamura, Masatsugu Toyota, Masao Tasaka, and Miyo Terao Morita
7. Auxin Carrier and Signaling Dynamics During Gravitropic Root GrowthMugurel I. Feraru, Jurgen Kleine-Vehn, and Elena Feraru
8. Imaging and Quantitative Methods for Studying Cytoskeletal Rearrangements during Root Development and Gravitropism
Eveline Jacques, Darren Wells, Malcolm J. Bennett, and Kris Vissenberg
9. Methods for RNA Profiling of Gravi-Responding Plant Tissues
Jyoti Dalal, Eric Land, Naresh Vasani, Luyan He, Caroline Smith, Maria Rodriguez-Welsh, Imara Y. Perera, and Heike Sederoff
10. Proteomic Approaches and their Application to Plant Gravitropism
Proma Basu, Darron R. Luesse, and Sarah E. Wyatt
11. Assays for Root Hydrotropism and Response to Water Stress
Delfeena Eapen, Jesus J. Martinez, and Gladys I. Cassab
12. Evaluating Mechano-Transduction and Touch Responses in Plant Roots
Sarah J. Swanson, Richard Barker, Yonggeng Ye, and Simon Gilroy
13. Expressing and Characterizing Mechanosensitive Channels in Xenopus Oocytes
Grigory Maksaev and Elizabeth S. Haswell
14. Flowering Shoots of Ornamental Crops as a Model to Study Cellular and Molecular Aspects of Plant Gravitropism
Sonia Philosoph-Hadas, Haya Friedman, and Shimon Meir
15. Studying Molecular Changes During Gravity Perception and Response in a Single Cell
Ashley E. Cannon, Mari L. Salmi, Thomas J. Bushart, and Stanley J. Roux
16. Live Cell and Immuno-Labeling Techniques to Study Gravitational Effects on Single Plant Cells
Youssef Chebli and Anja Geitmann
17. Use of High Gradient Magnetic Fields to Evaluate Gravity Perception and Response Mechanisms in Plants and Algae
Karl H. Hasenstein
18. Use of Microgravity Simulators for Plant Biological Studies
Raul Herranz, Miguel A. Valbuena, Aranzazu Manzano, Khaled Youssef, and F. Javier Medina
19. Conducting Plant Experiments in Space
John Z. Kiss
20. Spaceflight Exploration in Plant Gravitational Biology
Anna-Lisa Paul and Robert J. Ferl
21. Hypergravity Experiments to Evaluate Gravity Resistance Mechanisms in Plants
Kouichi Soga, Sachiko Yano, Shouhei Matsumoto, and Takayuki Hoson
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