Single cell protein analysis : methods and protocols
著者
書誌事項
Single cell protein analysis : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1346）（Springer protocols）
Humana Press, c2015
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell level. The book also includes chapters covering a suite of biochemical and biophysical methods capable of making an entire gamut of proteomic measurements, including analysis of protein abundance or expression, protein interaction networks, post-translational modifications, translocation and enzymatic activity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, Single Cell Protein Analysis: Methods and Protocols is useful to researchers and students in biological and biomedical sciences who have an interest in proteomic measurements in cells.
目次
1. Single-Cell Western Blotting
Elly Sinkala and Amy E. Herr
2. A Microfluidic Device for Immunoassay-Based Protein Analysis of Single E. Coli Bacteria
Simone Stratz and Petra S. Dittrich
3. Enzyme-Linked ImmunoSpot (ELISpot) for Single Cell Analysis
Sylvia Janetzki and Rachel Rabin
4. Photocleavable DNA Barcoding Antibodies for Multiplexed Protein Analysis in Single Cells
Adeeti V. Ullal and Ralph Weissleder
5. Genome-Wide Analysis of Protein and mRNA Copy Numbers in Single Escherichia Coli Cells with Single-Molecule Sensitivity
Yuichi Taniguchi
6. Microfluidic Flow Cytometry for Single Cell Protein Analysis
Meiye Wu and Anup K. Singh
7. Microfluidic Image Cytometry for Single Cell Phenotyping of Human Pluripotent Stem Cells
Yasumasa Mashimo and Ken-Ichiro Kamei
8. Characterizing Phenotypes and Signaling Networks of Single Human Cells by Mass Cytometry
Nalin Leelatian, Kirsten E. Diggins, and Jonathan M. Irish
9. Multiplex Peptide-MHC Tetramer Staining with Mass Cytometry
Leong Mei Ling and Evan W. Newell
10. Imaging and Mapping of Tissue Constituents at the Single Cell Level Using MALDI MSI and Quantitative Laser Scanning Cytometry
Catherine M. Rawlins, Joseph P. Salisbury, Daniel R. Feldman, Sinan Isim, Nathalie Y.R. Agar, Ed Luther, and Jeffery N. Agar
11. SPLIFF: A Single Cell Method to Map Protein-Protein Interactions in Time and Space
Alexander Dunkler, Reinhild Roesler, Hans A. Kestler, Daniel Moreno, and Nils Johnsson
12. Microfluidic Proximity Ligation Assay for Profiling Signaling
Networks with Single-Cell Resolution
Matthias Blazek, Roland Zengerle, and Matthias Meier
13. Dynamics and Interactions of Individual Proteins in the Membrane of Single, Living Cells
Stephen Anthony, Amanda Carroll-Portillo, and Jerilyn Timlin
14. Microfluidics Enabled Enzyme Activity Measurement in Single Cells
Cinzia Tesauro, Rikke Frohlich, Magnus Stougaard, Yi-Ping Ho, and Birgitta R. Knudsen
15. Microfluidic Chemical Cytometry for Enzyme Assays of Single Cells
Livia Shehaj, Lorena Lazo de la Vega, and Michelle L. Kovarik
16. Quantitative Detection of Nucleocytoplasmic Transport of Native Proteins in Single Cells
Zhenning Cao and Chang Lu
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