Plasmodesmata : methods and protocols

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書誌事項

Plasmodesmata : methods and protocols

edited by Manfred Heinlein

（Methods in molecular biology / John M. Walker, series editor, 1217）（Springer protocols）

Humana Press, c2015

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注記

Includes bibliographic references and index

内容説明・目次

内容説明

Plasmodesmata: Methods and Protocols provides a unique compendium of currently applied methods to explore plasmodesmata (PD). Opening with overviews of PD structure and their function during development as well as the role of viruses as models for addressing cellular mechanisms in macromolecular trafficking and movement through PD, the book continues with detailed experimental protocols for imaging PD, the isolation and structural analysis of PD, the analysis of PD conductivity and regulation, and for studying cellular mechanisms by which macromolecules are targeted to PD and transported through the channel. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Plasmodesmata: Methods and Protocols delivers detailed information about specific practical procedures in order to enable their reproduction and application in PD-related research in laboratories around the world.

目次

Part I: Overview 1. Plasmodesmata: Channels for Intercellular Signaling During Plant Growth and Development Iris Sevilem, Shri Ram Yadav, and Yka Helariutta 2. Plasmodesmata: Channels for Viruses on the Move Manfred Heinlein Part II: Imaging of Plasmodesmata 3. Imaging Plasmodesmata with High Resolution Scanning Electron Microscopy Deborah A. Barton and Robyn L. Overall 4. Preparative Methods for Imaging Plasmodesmata at Super-Resolution Karen Bell and Karl Oparka Part III: Structural Analysis of Plasmodesmata 5. Isolation of Plasmodesmata from Arabidopsis Suspension Culture Cells Magali S. Grison, Lourdes Fernandez-Calvino, Sebastien Mongrand, and Emmanuelle M.F. Bayer 6. Immunofluorescence Detection of Callose Deposition around Plasmodesmata Sites Ali Pendle and Yoselin Benitez-Alfonso 7. Imaging Callose at Plasmodesmata Using Aniline Blue: Quantitative Confocal Microscopy Raul Zavaliev and Bernard L. Epel 8. Localization of Fluorescently-Tagged Protein to Plasmodesmata by Correlative Light and Electron Microscopy Shannon Modla, Jeffrey L. Caplan, Kirk J. Czymmek, and Jung-Youn Lee Part IV: Analysis of Plasmodesmata Conductivity and Regulation 9. Quantification of Plant Cell Coupling with Live-Cell Microscopy Johannes Liesche and Alexander Schulz 10. Drop-ANd-See: A Simple, Real-Time, and Non-Invasive Technique for Assaying Plasmodesmal Permeability Weier Cui, Xu Wang, and Jung-Youn Lee 11. Mapping Symplasmic Fields at the Shoot Apical Meristem Using Iontophoresis and Membrane Potential Measurements Christiaan van der Schoot and Paivi L.H. Rinne 12. Analysis of the Conductivity of Plasmodesmata by Microinjection Friedrich Kragler 13. Investigating Plasmodesmata Genetics with Virus-Induced Gene Silencing and an Agrobacterium-Mediated GFP Movement Assay Jacob O. Brunkard, Tessa M. Burch-Smith, Anne M. Runkel, and Patricia Zambryski 14. Probing Plasmodesmata Function with Biochemical Inhibitors Rosemary G. White Part V: Studying Macromolecular Trafficking 15. GAL4 Transactivation-Based Assay for the Detection of Selective Intercellular Protein Movement Dhinesh Kumar, Huan Chen, Yeonggil Rim, and Jae-Yean Kim 16. Techniques for Assessing the Effects of Pharmacological Inhibitors on Intercellular Protein Movement Shuang Wu and Kimberly L. Gallagher 17. Probing Protein Targeting to Plasmodesmata Using Fluorescence Recovery after Photo-Bleaching Kathryn M. Wright and Katrin M. MacKenzie 18. The Tracking of Intercellular Small RNA Movement Christophe Himber and Patrice Dunoyer 19. Analysis of the Role of Myosins in Targeting Proteins to Plasmodesmata Martin Di Donato and Khalid Amari 20. Pumilio-Based RNA In Vivo Imaging Jens Tilsner 21. In Vivo RNA Labeling Using MS2 Eduardo Pena, Manfred Heinlein, and Adrian Sambade

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