Functional analysis of DNA and chromatin

著者

• Stockert, Juan C.
• Espada, Jesús
• Blázquez-Castro, Alfonso

書誌事項

Functional analysis of DNA and chromatin

edited by Juan C. Stockert, Jesús Espada, Alfonso Blázquez-Castro

（Methods in molecular biology / John M. Walker, series editor, v. 1094）（Springer protocols）

Humana Press, c2014

注記

Includes bibliographical references and index

内容説明・目次

内容説明

In Functional Analysis of DNA and Chromatin, expert researchers in the field provide an overview of standard and more recent methods for the functional analysis of the genetic material. These include methods on DNA-dye binding modes, chromatin staining, nuclear dispersion assays, DNA labeling in vivo, sister chromatid exchanges, FISH, DNA and chromatin imaging by fluorescence, electron and atomic force microscopy, detection of apoptotic DNA, cytosine methylation and hydroxy-methylation, DNA thermophoresis, improved methods for histone analysis, chromatin immunoprecipitation, and analysis of rDNA genes and chromatin-associated RNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Functional Analysis of DNA and Chromatin seeks to aid scientists in the further study of cellular and molecular biology of the nucleus.

目次

1. Predictive Binding Geometry of Ligands to DNA Minor Groove: Isohelicity and Hydrogen-Bonding Pattern Juan C. Stockert 2. Using Microchip Gel Electrophoresis to Probe DNA-Drug Binding Interactions Nan Shi and Victor M. Ugaz 3. Identifying Different Types of Chromatin using Giemsa Staining Juan C. Stockert, Alfonso Blazquez-Castro, and Richard W. Horobin 4. Analysis of DNA Damage and Repair by Comet Fluorescence in situ Hybridization (Comet-FISH) M. Glei and W. Schloermann 5. Alkaline Nuclear Dispersion Assays for the Determination of DNA Damage at the Single Cell Level Piero Sestili, and Carmela Fimognari 6. Polarization Microscopy of Extended Chromatin Fibres Maria Luiza S. Mello and Benedicto de Campos Vidal 7. DNA Labeling in Vivo: Quantification of Epidermal Stem Cell Chromatin Content in Whole Mouse Hair Follicles Using Fiji Image Processing Software Elisa Carrasco, Maria I. Calvo, and Jesus Espada 8. A Historical Overview of Bromo-Substituted DNA and Sister Chromatid Differentiation Roberto Mezzanotte and Mariella Nieddu 9. Image Analysis of Chromatin Remodelling Benedicto de Campos Vidal, PhD, Marina B. Felisbino, MS, Maria Luiza S. Mello 10. FISH Methods in Cytogenetic Studies Miguel Pita, Juan Orellana, Paloma Martinez-Rodriguez, Angel Martinez-Ramirez, Begona Fernandez-Calvin, and Jose L. Bella 11. Ultrastructural and Immunofluorescent Methods for the Study of the XY Body as a Biomarker Roberta B. Sciurano and Alberto J. Solari 12. Atomic Force Microscopy for Analyzing Metaphase Chromosomes. Comparison of AFM Images with Fluorescence Labeling Images of Banding Patterns Osamu Hoshi, and Tatsuo Ushiki 13. Selective Detection of Phagocytic Phase of Apoptosis in Fixed Tissue Sections Vladimir V. Didenko 14. Selective Transport of Cationized Fluorescent Topoisomerase into Nuclei of Live Cells for DNA Damage Studies Candace L. Minchew, and Vladimir V. Didenko 15. Visualization and Interpretation of Eukaryotic DNA Replication Intermediates in vivo by Electron Microscopy Kai J. Neelsen, Arnab Ray Chaudhuri, Cindy Follonier, Raquel Herrador, and Massimo Lopes 16. Combined Bi-dimensional Electrophoresis and Electron Microscopy to Study Specific Plasmid DNA Replication Intermediates in Human Cells Cindy Follonier and Massimo Lopes 17. Standard DNA Methylation Analysis in Mouse Epidermis: Bisulfite Sequencing, Methylation-Specific PCR, and 5-Methyl-Cytosine (5mC) Immunological Detection Jesus Espada, Elisa Carrasco, and Maria I. Calvo 18. Methyl-combing-Single Molecule Analysis of DNA Methylation on Stretched DNA Fibers Attila Nemeth 19. Investigating 5-Hydroxymethylcytosine (5hmC): The State of the Art Colm E. Nestor, James P. Reddington, Mikael Benson, and Richard R. Meehan 20. Hydroxymethylated DNA Immunoprecipitation (hmeDIP) Colm E. Nestor and Richard R. Meehan 21. Microscale Thermophoresis for the Assessment of Nuclear Protein Binding Affinities Wei Zhang, Stefan Duhr, Philipp Baaske, and Ernest Laue22. Analysis of Histone Post-translational Modifications from Nucleolus-associated Chromatin by Mass Spectrometry 23. Salt-Urea, Sulfopropyl-Sepharose, and Covalent Chromatography Methods for Histone Isolation and Fractionation Pedro Rodriguez-Collazo, Sanford Leuba, and Jordanka Zlatanova 24. Chromatin Immunoprecipitation Javier Rodriguez-Ubreva, and Esteban Ballestar 25. Analysis of Chromatin Composition of Repetitive Sequences: the ChIP-chop Assay Raffaella Santoro 26. Purification of Specific Chromatin Domains from Single-Copy Gene Loci in Saccharomyces cerevisiae Stephan Hamperl, Christopher Brown, Jorge Perez-Fernandez, Katharina Huber, Manuel Wittner, Virginia Babl, Ulrike Stoeckl, Hinrich Boeger, Herbert Tschochner, Philipp Milkereit, and Joachim Griesenbeck 27. Deep Sequencing of Small Chromatin-Associated RNA - Isolation and Library Preparation Sarah Diermeier, Thomas Schubert, and Gernot Langst 28. Deep Sequencing of Small Chromatin-Associated RNA - Bioinformatic Analysis Sarah Diermeier and Gernot Langst

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詳細情報

• NII書誌ID(NCID): BB21220094
• ISBN:
  • 9781627037051
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xv, 365 p.
• 大きさ: 27 cm
• 分類:
  • DC23 : 572.86
• 件名:
  • LCSH : DNA -- Analysis
  • LCSH : Chromatin -- Analysis
• 親書誌ID:
  • BA10794673
  • BA88873632