P-type ATPases : methods and protocols

著者

• Bublitz, Maike

書誌事項

P-type ATPases : methods and protocols

edited by Maike Bublitz

（Methods in molecular biology / John M. Walker, series editor, 1377）

Humana Press, c2016

注記

Includes bibliographical references and index

Also published electronically

内容説明・目次

内容説明

This volume provides a broad collection of protocols for many of the common experimental techniques used for the characterization of P-type ATPases. P-Type ATPases: Methods and Protocols provides comprehensive practical instructions for all researchers in the P-type ATPase field, from the protein biochemist to the mouse geneticist, covering the identification and classification, isolation, purification, in vitro characterization, knock-out studies, as well as crystallization and structural analysis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, P-Type ATPases: Methods and Protocols aims to ensure successful results in the further study of this vital field of research.

目次

1. An Introduction to P-type ATPase research Poul Nissen Part I: Protein Production, Isolation, Purification, and Stabilization 2. Purification of Na,K-ATPase from Pig Kidney Natalya U. Fedosova 3. Preparation of Ca2+-ATPase1a Enzyme from Rabbit Sarcoplasmic Reticulum Jesper V. Moller and Claus Olesen 4. Isolation of H+/K+-ATPase-enriched Membrane Fraction from Pig Stomachs Kazuhiro Abe and Claus Olesen 5. Overproduction of PiB-type ATPases Xiangyu Liu, Oleg Sitsel, Kaituo Wang, and Pontus Gourdon 6. Coordinated Overexpression in Yeast of a P4-ATPase and its Associated Cdc50 Subunit: The Case of the Drs2p/Cdc50p Lipid Flippase Complex Hassina Azouaoui, Cedric Montigny, Aurore Jacquot, Raphaelle Barry, Philippe Champeil, and Guillaume Lenoir 7. The Plasma Membrane Ca2+-ATPase: Purification by Calmodulin Affinity Chromatography, and Reconstitution of the Purified Protein Verena Niggli and Ernesto Carafoli 8. Expression of Na,K-ATPase and H,K-ATPase Isoforms With The Baculovirus Expression System Jan B. Koenderink and Herman G.P. Swarts 9. Time-dependent Protein Thermostability Assay Ilse Vandecaetsbeek and Peter Vangheluwe Part II : Activity Assays 10. Colorimetric Assays of Na,K-ATPase Kathleen J. Sweadner 11. ATPase Activity Measurements by an Enzyme-Coupled Spectrophotometric Assay Pankaj Sehgal, Claus Olesen, and Jesper V. Moller 12. Antimony-Phosphomolybdate ATPase Assay Gianluca Bartolommei and Francesco Tadini-Buoninsegni 13. ATPase Activity Measurements using radiolabeled ATP Herman G.P. Swarts and Jan B. Koenderink 14. Assaying P-type ATPases Reconstituted in Liposomes Hans-Jurgen Apell and Bojana Damnjanovic 15. Coupling ratio for Ca2+ Transport by Calcium Oxalate Precipitation Pankaj Sehgal, Claus Olesen, and Jesper V. Moller 16. Calcium Uptake in Crude Tissue Preparation Philip A. Bidwell and Evangelia G. Kranias 17. Measuring H+ Pumping and Membrane Potential Formation in Sealed Membrane Vesicle Systems Alex Green Wielandt, Michael G. Palmgren, Anja Thoe Fuglsang, Thomas Gunther Pomorski, and Bo Hojen Justesen 18. Assay of Flippase Activity in Proteoliposomes using Fluorescent Lipid Derivatives Magdalena Marek and Thomas Gunther Pomorski Part III : In vitro Functional Studies 19. The use of Metal Fluoride Compounds as Phosphate Analogs for Understanding the Structural Mechanism in P-type ATPases Stefania J. Danko and Hiroshi Suzuki 20. Phosphorylation/Dephosphorylation Assays Hiroshi Suzuki 21. Tryptophan Fluorescence Changes Related to Ca2+-ATPase Function Pankaj Sehgal, Claus Olesen, and Jesper V. Moller Part IV: Ligand Binding Studies 22. Determination of the ATP Affinity of the Sarcoplasmic Reticulum Ca2+-ATPase by Competitive Inhibition of [g-32P]TNP-8N3-ATP Photolabeling Johannes D. Clausen, David B. McIntosh, David G. Woolley, and Jens Peter Andersen 23. Ca2+ Binding and Transport Studied with Ca2+/EGTA Buffers and 45Ca2+ Pankaj Sehgal, Claus Olesen, and Jesper V. Moller 24. Assay of Copper Transfer and Binding to P1B-ATPases Teresita Padilla-Benavides and Jose M. Arguello Part V: Electrophysiology 25. Voltage Clamp Fluorometry of P-Type ATPases Robert E. Dempski 26. Electrophysiological Measurements on Solid Supported Membranes Francesco Tadini-Buoninsegni and Gianluca Bartolommei 27. Electrophysiological Characterization of Na,K-ATPases Expressed in Xenopus laevis Oocytes Using Two-Electrode Voltage Clamping Florian Hilbers and Hanne Poulsen Part VI: Functional Studies by Cell Culture and Transgenic Animals 28. Functional Studies of Na+,K+-ATPase using Transfected Cell Cultures Elena Arystarkhova and Kathleen J. Sweadner 29. HPLC Neurotransmitter Analysis Thomas Hellesoe Holm, Toke Jost Isaksen, and Karin Lykke-Hartmann 30. Behavior Test Relevant to a2/a3Na+/K+-ATPase Gene Modified Mouse Models Toke Jost Isaksen, Thomas Hellesoe Holm, and Karin Lykke-Hartmann 31. Zebrafish Whole-mount in situ Hybridization Followed by Sectioning Canan Doganli, Jens Randel Nyengaard, and Karin Lykke-Hartmann 32. Whole-mount Immunohistochemistry for Anti-F59 in Zebrafish Embryos (1-5 days Post Fertilization (dpf)) Canan Doganli Lucas Bukata and Karin Lykke-Hartmann 33. Cell-based Lipid Flippase Assay Employing Fluorescent Lipid Derivatives Maria S. Jensen, Sara Costa, Thomas Gunther-Pomorski, and Rosa L. Lopez-Marques 34. Transient Expression of P-type ATPases in Tobacco Epidermal Cells Lisbeth R. Poulsen, Michael G. Palmgren, and Rosa L. Lopez-Marques Part VII: Lipid Techniques 35. Lipid Exchange by Ultracentrifugation Nikolaj D. Drachmann and Claus Olesen 36. Reconstitution of Na+, K+-ATPase in Nanodiscs Jonas Lindholt Gregersen, Natalya Fedosova, Poul Nissen, and Thomas Boesen Part VIII: Crystallization 37. Crystallization of P-type ATPases by the High Lipid-detergent (HiLiDe) Method Oleg Sitsel, Kaituo Wang, Xiangyu Liu, and Pontus Gourdon 38. Two-dimensional Crystallization of the Ca2+-ATPase for Electron Crystallography John Paul Glaves, Joseph O. Primeau, and Howard S. Young 39. Two-dimensional Crystallization of Gastric H+,K+-ATPase for Structural Analysis By Electron Crystallography Kazuhiro Abe Part IX: Computational Approaches in analyzing P-type ATPases 40. MD Simulations of P-type ATPases in a Lipid Bilayer System Henriette Elisabeth Autzen and Maria Musgaard 41. Computational Classification of P-Type ATPases Dan Sondergaard, Michael Knudsen, and Christian Norgaard Storm Pedersen 42. Molecular Modeling of Fluorescent SERCA Biosensors Bengt Svensson, Joseph M. Autry, and David D. Thomas 43. How to Compare, Analyze and Morph Between Crystal Structures of Different Conformations: The P-type ATPase Example Jesper L. Karlsen and Maike Bublitz

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詳細情報

• NII書誌ID(NCID): BB21543049
• ISBN:
  • 9781493931781
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xiv, 544 p.
• 大きさ: 27 cm
• 分類:
  • DC23 : 572.696
• 件名:
  • LCSH : Carrier proteins
  • LCSH : Ion pumps
• 親書誌ID:
  • BA10794673