T follicular helper cells : methods and protocols
著者
書誌事項
T follicular helper cells : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1291）（Springer protocols）
Humana Press, c2015
大学図書館所蔵 件 / 全1件
-
該当する所蔵館はありません
- すべての絞り込み条件を解除する
注記
Includes bibliographical references and index
内容説明・目次
内容説明
This volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Concise and easy-to-use, T follicular Helper Cells: Methods and Protocols provides scientist with techniques and protocols that have facilitated breakthroughs in Tfh biology and to present them in a way that will enable both new and experienced researchers to continue to move this exciting field forward.
目次
Part I: Mouse
1. Identification of Mouse T Follicular Helper Cells by Flow Cytometry
Alexandre Meli & Irah King
2. Identification of Follicular T Helper Cells in Tissue Sections
Yanna Ding, John D. Mountz, and Hui-Chen Hsu
3. Tracking Early T Follicular Helper Cell Differentiation In vivo
Dirk Baumjohann and K. Mark Ansel
4. Tracking by Flow Cytometry Antigen-Specific Follicular Helper T-Cells In Wild-Type Animals After Protein Vaccination
Svetoslav Chakarov and Nicolas Fazilleau
5. Retroviral Vector Expression in TCR Transgenic CD4+ T Cells
Youn Soo Choi and Shane Crotty
6. Two-photon Microscopy For Imaging Germinal Centres and T Follicular Helper Cells
Menna R. Clatworthy
7. In vivo Induction of T-follicular Helper Cells by Modulation of Regulatory T Cell Function
James B. Wing and Shimon Sakaguchi
8. Assessing T Follicular Helper Cell Function in vivo: Antigen-specific B Cell Response to Hapten and Affinity Maturation
Jessica Natt and Marion Espeli
9. The SWHEL System For High-resolution Analysis of in vivo Antigen-specific T-Dependent B Cell Responses
R. Brink, D. Paus, K. Bourne, J.R. Hermes, S. Gardam, T.G. Phan, and T.D. Chan
10. Triggering Positive Selection of Germinal Center B cells by Antigen Targeting to DEC-205
Giulia Pasqual, Alessandro Angelini, and Gabriel D. Victora
11. Detection of Mouse Natural Killer T Follicular Helper (NKTFH) Cells by Flow Cytometry
Pheh-Ping Chang and Carola G. Vinuesa
12. Identification of Foxp3+ T Follicular Regulatory (Tfr) Cells
by Flow Cytometry
Ana Raquel Maceiras and Luis Graca
13. In vitro Assay to Sensitively Measure Tfr Suppressive Capacity and Tfh Stimulation of B Cell Responses
Peter T. Sage and Arlene H. Sharpe
Part II: Human
14. Flow Cytometric Detection and Isolation of Human Tonsil or Lymph Node T Follicular Helper Cells
Jan Misiak, Karin Tarte, and Patricia Ame-Thomas
15. Human Tfh and Tfr cells: Identification and Assessment of their Migration Potential
Chang H. Kim, Seika Hashimoto-Hill, and Seung G. Kang
16. Analysis of Human Blood Memory T Follicular Helper Subsets
Salah-Eddine Bentebibel, Clement Jacquemin, Nathalie Schmitt, and Hideki Ueno
17. Flow Cytometric Analysis of Circulating Follicular Helper T (Tfh) and Follicular Regulatory T (Tfr) Populations in Human Blood
Yunbo Wei, Jinghong Feng, Zhaohua Hou, Xin Maggie Wang, and Di Yu
18. Quantifying Helper Cell Function of Human TFH Cells in vitro
Nina Chevalier
「Nielsen BookData」 より