Isolation of plant organelles and structures : methods and protocols

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書誌事項

Isolation of plant organelles and structures : methods and protocols

edited by Nicolas L. Taylor; A. Harvey Millar

（Methods in molecular biology / John M. Walker, series editor, 1511）（Springer protocols）

Humana Press, c2017

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This book brings together the major techniques used in the isolation or enrichment of individual populations of organelles and other subcellular structures from plants with the goal that, by being able to isolate subcellular structures, the research and understanding of various facets of compartmentalized function in plant cells can be advanced. Written for the highly successful Methods in Molecular Biology series, expert contributors provide chapters that contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Isolation of Plant Organelles and Structures: Methods and Protocols will greatly aid those who regularly isolate subcellular components as well as those whose research has lead them to focus on a subcellular compartment or a particular process for the first time, thus producing the need to be able to isolate it or enrich it for study.

目次

1. The Isolation of Plant Organelles and Structures in the Post-Genomics Era A. Harvey Millar and Nicolas L. Taylor 2. Approaches to Characterize Organelle, Compartment, or Structure Purity Stefanie J. Mueller, Sebastian N.W. Hoernstein, and Ralf Reski Part I: Isolation of Organelles and Organelle Compartments < 3. Isolation of Nuclei and Nucleoli Alison F. Pendle and Peter J. Shaw 4. Isolation and Suborganellar Fractionation of Arabidopsis Chloroplasts Ursula Flores-Perez and Paul Jarvis 5. Isolation of Chromoplasts and Sub-Organellar Compartments from Tomato and Bell Pepper Fruit Cristina Barsan, Marcel Kuntz, and Jean-Claude Pech 6. Leucoplast Isolation and Subfractionation William C. Plaxton 7. Isolation of Mitochondria, Their Sub-Organellar Compartments, and Membranes Owen Duncan, A. Harvey Millar, and Nicolas L. Taylor 8. Isolation of Arabidopsis Leaf Peroxisomes and the Peroxisomal Membrane Sigrun Reumann and Piotr Lisik 9. Isolation of Vacuoles and the Tonoplast Jan Zouhar 10. Isolation of Endoplasmic Reticulum and Its Membrane G. Eric Schaller 11. Enrichment of Golgi Membranes from Triticum aestivum (Wheat) Seedlings Wei Zeng, Berit Ebert, Harriet T. Parsons, Carsten Rautengarten, Antony Bacic, and Joshua L. Heazlewood 12. Isolation of Autolysosomes from Tobacco BY-2 Cells Chihiro Takatsuka, Yuko Inoue, and Yuji Moriyasu 13. Isolation of Protein Storage Vacuoles and Their Membranes Tomoo Shimada and Ikuko Hara-Nishimura Part II: Isolation of Other Structures and Compartments < 14. Isolation of the Cell Wall Herve Canut, Cecile Albenne, and Elisabeth Jamet 15. Isolation of Plasmodesmata Christine Faulkner and Emmanuelle M.F. Bayer 16. Isolation of Plasma Membrane and Plasma Membrane Microdomains Anzu Minami, Daisuke Takahashi, Yukio Kawamura, and Matsuo Uemura 17. Enrichment of the Plant Cytosolic Fraction Jeemeng Lao, Andreia M. Smith-Moritz, Jennifer C. Mortimer, and Joshua L. Heazlewood 18. Isolation of Apoplast Erik Andreasson, Kibrom B. Abreha, and Svante Resjoe 19. Isolation of Cytosolic Ribosomes Hanna Klang Arstrand and Maria V. Turkina 20. Isolation of Plastid Ribosomes Kenichi Yamaguchi 21. Isolation of Mitochondrial Ribosomes Adam J. Carroll 22. Isolation of Microtubules and Microtubule-Associated Proteins Takahiro Hamada and Seiji Sonobe 23. Isolation of Actin and Actin Binding Proteins Etsuo Yokota 24. Purification of 26S Proteasomes and Their Sub-Complexes from Plants Richard S. Marshall, David C. Gemperline, and Richard D. Vierstra

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