Isolation of plant organelles and structures : methods and protocols
著者
書誌事項
Isolation of plant organelles and structures : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, 1511)(Springer protocols)
Humana Press, c2017
大学図書館所蔵 全5件
  青森
  岩手
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  福島
  茨城
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  埼玉
  千葉
  東京
  神奈川
  新潟
  富山
  石川
  福井
  山梨
  長野
  岐阜
  静岡
  愛知
  三重
  滋賀
  京都
  大阪
  兵庫
  奈良
  和歌山
  鳥取
  島根
  岡山
  広島
  山口
  徳島
  香川
  愛媛
  高知
  福岡
  佐賀
  長崎
  熊本
  大分
  宮崎
  鹿児島
  沖縄
  韓国
  中国
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  イギリス
  ドイツ
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This book brings together the major techniques used in the isolation or enrichment of individual populations of organelles and other subcellular structures from plants with the goal that, by being able to isolate subcellular structures, the research and understanding of various facets of compartmentalized function in plant cells can be advanced. Written for the highly successful Methods in Molecular Biology series, expert contributors provide chapters that contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Isolation of Plant Organelles and Structures: Methods and Protocols will greatly aid those who regularly isolate subcellular components as well as those whose research has lead them to focus on a subcellular compartment or a particular process for the first time, thus producing the need to be able to isolate it or enrich it for study.
目次
1. The Isolation of Plant Organelles and Structures in the Post-Genomics Era
A. Harvey Millar and Nicolas L. Taylor
2. Approaches to Characterize Organelle, Compartment, or Structure Purity
Stefanie J. Mueller, Sebastian N.W. Hoernstein, and Ralf Reski
Part I: Isolation of Organelles and Organelle Compartments
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3. Isolation of Nuclei and Nucleoli
Alison F. Pendle and Peter J. Shaw
4. Isolation and Suborganellar Fractionation of Arabidopsis Chloroplasts
Ursula Flores-Perez and Paul Jarvis
5. Isolation of Chromoplasts and Sub-Organellar Compartments from Tomato and Bell Pepper Fruit
Cristina Barsan, Marcel Kuntz, and Jean-Claude Pech
6. Leucoplast Isolation and Subfractionation
William C. Plaxton
7. Isolation of Mitochondria, Their Sub-Organellar Compartments, and Membranes
Owen Duncan, A. Harvey Millar, and Nicolas L. Taylor
8. Isolation of Arabidopsis Leaf Peroxisomes and the Peroxisomal Membrane
Sigrun Reumann and Piotr Lisik
9. Isolation of Vacuoles and the Tonoplast
Jan Zouhar
10. Isolation of Endoplasmic Reticulum and Its Membrane
G. Eric Schaller
11. Enrichment of Golgi Membranes from Triticum aestivum (Wheat) Seedlings
Wei Zeng, Berit Ebert, Harriet T. Parsons, Carsten Rautengarten, Antony Bacic, and Joshua L. Heazlewood
12. Isolation of Autolysosomes from Tobacco BY-2 Cells
Chihiro Takatsuka, Yuko Inoue, and Yuji Moriyasu
13. Isolation of Protein Storage Vacuoles and Their Membranes
Tomoo Shimada and Ikuko Hara-Nishimura
Part II: Isolation of Other Structures and Compartments
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14. Isolation of the Cell Wall
Herve Canut, Cecile Albenne, and Elisabeth Jamet
15. Isolation of Plasmodesmata
Christine Faulkner and Emmanuelle M.F. Bayer
16. Isolation of Plasma Membrane and Plasma Membrane Microdomains
Anzu Minami, Daisuke Takahashi, Yukio Kawamura, and Matsuo Uemura
17. Enrichment of the Plant Cytosolic Fraction
Jeemeng Lao, Andreia M. Smith-Moritz, Jennifer C. Mortimer, and Joshua L. Heazlewood
18. Isolation of Apoplast
Erik Andreasson, Kibrom B. Abreha, and Svante Resjoe
19. Isolation of Cytosolic Ribosomes
Hanna Klang Arstrand and Maria V. Turkina
20. Isolation of Plastid Ribosomes
Kenichi Yamaguchi
21. Isolation of Mitochondrial Ribosomes
Adam J. Carroll
22. Isolation of Microtubules and Microtubule-Associated Proteins
Takahiro Hamada and Seiji Sonobe
23. Isolation of Actin and Actin Binding Proteins
Etsuo Yokota
24. Purification of 26S Proteasomes and Their Sub-Complexes from Plants
Richard S. Marshall, David C. Gemperline, and Richard D. Vierstra
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