Plant mitochondria : methods and protocols
Author(s)
Bibliographic Information
Plant mitochondria : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1305）（Springer protocols）
Humana Press, c2015
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
The chapters compiled in this detailed collection outline a number of methods used to study plant mitochondria today, starting from the isolation of mitochondria to detailed analyses of RNA, protein and enzymatic activities. Given that the ability to uncover mitochondria's unique features is underpinned by current methodology, this book explores the subject from morphology to detailed molecular mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Plant Mitochondria: Methods and Protocols serves as a vital resource to beginners in the field as well as to expert researchers who find themselves being pulled into the field of mitochondrial research as it links to so many important aspects of plant cell biology.
Table of Contents
1. Isolation of Intact Mitochondria from the Model Plant Species Arabidopsis thaliana and Oryza sativa
Monika W. Murcha and James Whelan
2. Determining Mitochondrial Transcript Termini for the Study of Transcription Start Sites and Transcript 5' End Maturation
Stefan Binder and Kristina Kuhn
3. Mitochondrial Run-On Transcription Assay Using Biotin Labeling
Kristina Kuhn
4. In Vitro RNA Uptake Studies in Plant Mitochondria
Szymon Kubiszewski-Jakubiak, Cyrille Megel, Elodie Ubrig, Thalia Salinas, Anne-Marie Duchene, and Laurence Marechal-Drouard
5. In Vitro and In Vivo Protein Uptake Studies in Plant Mitochondria
Owen Duncan, Chris Carrie, Yan Wang, and Monika W. Murcha
6. Plant Mitochondrial Proteomics
Nicolas L. Taylor and A. Harvey Millar
7. Identification of Lysine-Acetylated Mitochondrial Proteins and Their Acetylation Sites
Markus Hartl, Ann-Christine Koenig, and Iris Finkemeier
8. A Flowchart to Analyze Protease Activity in Plant Mitochondria
Pedro F. Teixeira, Rui M. Branca, Beata Kmiec, and Elzbieta Glaser
9. Activity Measurements of Mitochondrial Enzymes in Native Gels
Peter Schertl and Hans-Peter Braun
10. Activity Assay for Plant Mitochondrial Enzymes
Shaobai Huang, Chun Pong Lee, and A. Harvey Millar
11. Analysis of Type II NAD(P)H Dehydrogenases
Kathleen L. Soole and Chevaun A. Smith
12. Assessment of Respiration in Isolated Plant Mitochondria Using Clark Type Electrodes
Richard P. Jacoby, A. Harvey Millar, and Nicolas L. Taylor
13. Micro-Respiration Measurements in Plants
Yun Shin Sew, A. Harvey Millar, and Elke Stroeher
14. Improvements to Define Mitochondrial Metabolomics Using Non Aqueous Fractionation
Richard Fly, James Lloyd, Stephan Krueger, Alisdair Fernie, and Margaretha J. van der Merwe
15. Mitochondrial Markers of Programmed Cell Death in Arabidopsis thaliana
Theresa J. Reape, Joanna Kacprzyk, Niall Brogan, Lee Sweetlove, and Paul F. McCabe
16. Imaging and Analysis of Mitochondrial Dynamics in Living Cells
Sanjaya B. Ekanayake, Amr M. El Zawily, Gael Paszkiewicz, Aurelia Rolland, and David C. Logan
17. Analysis of Plant Mitochondrial Function Using Fluorescent Protein Sensors
Stephan Wagner, Thomas Nietzel, Isabel Aller, Alex Costa, Mark D. Fricker, Andreas J. Meyer, and Markus Schwarzlander
18. In Planta Analysis of Leaf Mitochondrial Superoxide and Nitric Oxide
Marina Cvetkovska and Greg C. Vanlerberghe
19. Databases and Informatics Resources For Analysis of Plant Mitochondria
Reena Narsai
20. Expression and Crystallization of the Plant Alternative Oxidase
Benjamin May, Catherine Elliott, Momi Iwata, Luke Young, Julia Shearman, Mary S. Albury, and Anthony L. Moore
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