Plant gene silencing : methods and protocols

Author(s)

• Mysore, Kirankumar S
• Senthil-Kumar, Muthappa

Bibliographic Information

Plant gene silencing : methods and protocols

edited by Kirankumar S. Mysore, Muthappa Senthil-Kumar

（Methods in molecular biology / John M. Walker, series editor, 1287）（Springer protocols）

Humana Press, c2015

• : pbk

Note

Includes bibliographical references and index

Description and Table of Contents

Description

This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Plant Gene Silencing: Methods and Protocols seeks to aid scientists to further understand functional relevance of target genes using gene silencing methods and use these technologies in commercial plant varieties.

Table of Contents

Part I Review Chapters 1. Advanced in Plant Gene Silencing Methods Prachi Pandey, Muthappa Senthil-Kumar, and Kirankumar S. Mysore 2. Strategies for Altering Plant Traits Using Virus-Induced Gene Silencing Technologies Christophe Lacomme 3. Bioinformatics Tools for Achieving Better Gene Silencing in Plants Firoz Ahmed, Xinbin Dai, and Patrick Xuechun Zhao 4. Profiling of Small RNAs Involved in Plant-Pathogen Interactions Dongdong Niu, Zhaoyun Wang, Shune Wang, Lulu Qiao, and Hongwei Zhao 5. RNAi-Mediated Resistance to Viruses in Genetically Engineered Plants Abdulrazak B. Ibrahim and Francisco J. L. Aragao Part II Methods Chapter 6. Simplifying Transgene Locus Structure Through Cre-lox Recombination Vibha Srivastava and David W. Ow 7. Transgene-Induced Gene Silencing in Plants Yun Jin and Hui-Shan Guo 8. Gene Silencing by DNA Interference in Fern Gametophytes Masamitsu Wada and Hidenori Tsuboi9. Induction of Stable Epigenetic Gene Silencing in Plants Using a Virus Vector Akira Kanazawa and Megumi Kasai 10. A Method for Validating MicroRNAs in Plants by miR-RACE Jinggui Fang and Xin Sun 11. MR VIGS: MicroRNA-Based Virus-Induced Gene Silencing in Plants Weiwei Chen, Qj Zhang, Junhua Kong, Feng Hu, Bin Li, Chaoqun Wu, Cheng Qin, Pencheng Zhang, Nongnong Shi, and Yiguo Hong 12. A High-Throughput RNA Interference (RNAi)-Based Approach Using Hairy Roots for the Study of Plant-Rhizobia Interactions Senjuti Sinharoy, Catalina I. Pislariu, and Michael K. Udvardi 13. A Functional Genomics Method for Assaying Gene Function in Phytopathogenic Fungi Through Host-Induced Gene Silencing Mediated by Agroinfiltration Vinay Panwar, Brent McCallum, and Guus Bakkeren 14. An Effective and Convenient Method for the Delivery of Apple Latent Spherical Virus (ALSV)-Based Vectors into Plant Cells by Agroinoculation Tatsuya Kon and Nubuyuki Yoshikawa 15. Virus-Induced Gene Silencing (VIGS) for Functional Genomics in Rice Using rice tungro bacilliform virus (RTBV) as a Vector Ravi Kant, Shweta Sharma, and Indranil Dasgupta 16. Virus-Induced Gene Silencing of Fiber-Related Genes in Cotton John R. Tuttle, Candace H. Haigler, and Dominique Robertson 17. Establishment of an Efficient Virus-Induced Gene Silencing (VIGS) Assay in Arabidopsis by Agrobacterium-Mediated Rubbing Infection Ana Marcia E. de A. Manhaes, Marcos V. V. de Oliveira, and Libo Shan 18. Virus-Induced Gene Silencing as a Scalable Tool to Study Drought Tolerance in Plants Gavin M. George, Michael E. Ruckle, and James R. Lloyd 19. VIGS for Dissecting Mechanisms Involved in the Symbiotic Interaction of Microbes with Plants Mette Gronlund 20. Construction of a Cotton VIGS Library for Functional Genomic Study Maoying Li, Fangjun Li, and Ping He 21. Synthetic Gene Complementation to Determine Off-Target Silencing Dhirendra Kumar22. Construction of Mismatched Inverted Repeat (IR) Silencing Vectors for Maximizing IR Stability and Effective Gene Silencing and in Plants M. E. Chrissie Rey, Johan Harmse, Sarah H. Taylor, Patrick Arbuthnot, and Marc S. Weinberg

by "Nielsen BookData"

Details

• NCID: BB22824280
• ISBN:
  • 9781493924523
  • 9781493948475
• LCCN: 2015930614
• Country Code: us
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: New York
• Pages/Volumes: xi, 307 p.
• Size: 27 cm
• Classification:
  • LCC : QK981.4
  • NLM : QU 25
  • NLM : W1
  • NLM : QK 25
  • DC23 : 572.8
  • DC20 : 581.8
• Subject Headings:
  • LCSH : Plant gene silencing -- Laboratory manuals
  • MESH : Gene Silencing -- Laboratory Manuals
  • MESH : Gene Expression Regulation, Plant -- Laboratory Manuals
• Parent Bibliography ID:
  • BA10794673
  • BA88873632