Cyclin-dependent kinase (CDK) inhibitors : methods and protocols

著者

    • Orzáez, Mar
    • Sancho Medina, Mónica
    • Pérez-Payá, Enrique

書誌事項

Cyclin-dependent kinase (CDK) inhibitors : methods and protocols

edited by Mar Orzáez, Mónica Sancho Medina, Enrique Pérez-Payá

（Methods in molecular biology / John M. Walker, series editor, 1336）（Springer protocols）

Humana Press, c2016

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This volume contains a collection of relevant information for drug discovery in cell cycle research. Protocols to develop screening assays or to identify novel CDK inhibitors are discussed in the first part of the book. The second part of the book describes elaborate procedures to evaluate activity and mechanism of action of new and already identified CDK inhibitors. The third part of the book talks about protocols to evaluate metabolomics changes associated to inhibitor treatment. Drug delivery strategies focused on nanoparticle development to provide alternative internalization systems for increasing inhibitor efficacy are also described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Cyclin-Dependent Kinase (CDK) Inhibitors: Methods and Protocols is a useful tool for scientists interested in this research field.

目次

1. Immunoprecipitation of CDK-Cyclin Complexes for Determination of Kinase Activity Edurne Gallastegui and Oriol Bachs 2. Expression and Purification of Recombinant Cyclins and CDKs for Activity Evaluation Edurne Gallastegui and Oriol Bachs 3. Expression and Purification of Recombinant CDKs: CDK7, CDK8 and CDK9 Reena Pinhero and Krassimir Yankulov 4. Preparation of CDK/Cyclin Inhibitor Complexes for Structural Determination Asterios I. Grigoroudis and George Kontopidis 5. Fragment-Based De Novo Design of Cyclin-Dependent Kinase 2 Inhibitors Sunil Kumar Tripathi, Poonam Singh, and Sanjeev Kumar Singh 6. Protein-Protein Interaction Inspire the De Novo Design of Cyclin-Dependent Kinases Peptide Inhibitors Arumugasamy Karthiga, Sunil Kumar Tripathi, Poonam Singh, and Sanjeev Kumar Singh 7. Identification of Cyclin A Binders with a Fluorescent Peptide Sensor Elena Pazos, Jose L. Mascarenas and M. Eugenio Vazquez 8. Cell Synchronization Techniques to Study the Action of CDK Inhibitors Beatriz Perez-Benavente and Rosa Farras 9. Analysis of CDK Inhibitors Action on Mitochondria-Mediated Apoptosis Anna Gortat 10. Evaluating the Effects of CDK Inhibitors in Ischemia-Reperfusion Injury Models Tatiana Guevara 11. Assessing Cell Cycle Independent Function of the CDK Inhibitor p21CDKN1A in DNA Repair Ilaria Dutto, Micol Tillhon, and Ennio Prosperi 12. Drug Delivery Strategies of Chemical CDK Inhibitors Daniel Alvira and Laura Mondragon 13. Animal Models for Studying the In Vivo Functions of Cell Cycle CDKs Sanjiv Risal, Deepak Adhikari, and Kui Liu 14. Evaluating Chemical CDK Inhibitors as Cell Death Inducers Hiroshi Hirai and Yoko Nakatsuru 15. Models for the Study of the Crosstalk between Inflammation and Cell Cycle Laura J. Hoodless, Calum T. Robb, Jennifer M. Felton, Carl S. Tucker, and Adriano G. Rossi 16. Metabolomic Applications to the Characterization of the Mode-of-Action of CDK Inhibitors Martina Palomino-Schatzlein and Antonio Pineda-Lucena

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