PCR primer design
Author(s)
Bibliographic Information
PCR primer design
（Methods in molecular biology / John M. Walker, series editor, 1275）（Springer protocols）
Humana Press, c2015
2nd ed
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design, and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, PCR Primer Design, Second Edition seeks to aid molecular biology students, researchers, professors and PCR enthusiasts.
Table of Contents
1. Fast Masking Of Repeated Primer Binding Sites In Eukaryotic Genomes
Reidar Andreson, Lauris Kaplinski, and Maido Remm
2. Primer Design for PCR Reactions in Forensic Biology
Kelly M. Elkins
3. Design of Primers and Probes For Quantitative Real-Time PCR MethodsAlicia Rodriguez, Mar Rodriguez, Juan J. Cordoba, and Maria J. Andrade
4. Large-scale Nucleotide Sequence Alignment And Sequence Variability Assessment To Identify the Evolutionarily Highly Conserved Regions For Universal Screening PCR Assay Design: An Example Of Influenza A VirusNagy Alexander, Jirinec, Tomas, Jirincova, Helena, and Havlickova, Martina
5. Low-concentration Initiator Primers Improve The
Amplification Of Gene Targets With High Sequence Variability
Kenneth E. Pierce and Lawrence J. Wangh
6. Multiplex PCR Primer Design for Simultaneous Detection of Multiple Pathogens
Wenchao Yan
7. Degenerate Primer Design for Highly Variable Genomes
Kelvin Li, Susmita Shrivastava, and Timothy B. Stockwell
8. Allele-specific Real Time Polymerase Chain Reaction As A Tool For Urate Transporter 1 Mutation Detection
Juliet O. Makanga, Antonius Christianto, and Tetsuya Inazu
9. MultiPLX: Automatic Grouping And Evaluation Of PCR Primers
Lauris Kaplinski and Maido Remm
10. In silico PCR Primer Designing and Validation
Anil Kumar and Nikita Chordia
11. Primer Design using Primer Express (R) for SYBR Green Based Quantitative PCR
Amarjeet Singh and Girdhar K. Pandey
12. Designing Primers for SNaPshot Technique
Greiciane Gaburro Paneto and Francisco de Paula Careta
13. Rapid and Simple Method of qPCR Primer Design
Brenda Thornton and Chhandak Basu
14. PRIMEGENSw3: A Web-based Tool for High-throughput Primer and Probe Design
Garima Kushwaha
15. Selecting Specific PCR Primers with MFEprimerWubin Qu and Chenggang Zhang
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