Imaging flow cytometry : methods and protocols

著者

    • Barteneva, Natasha S
    • Vorobjev, Ivan A

書誌事項

Imaging flow cytometry : methods and protocols

edited by Natasha S. Barteneva, Ivan A. Vorobjev

（Methods in molecular biology / John M. Walker, series editor, 1389）（Springer protocols）

Humana Press, c2016

大学図書館所蔵 件 / 全2件

この図書・雑誌をさがす

注記

Includes bibliographical references and index

内容説明・目次

内容説明

This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.

目次

Part I: General 1. Quantitative Functional Morphology by Imaging Flow Cytometry Ivan A. Vorobjev and Natasha S. Barteneva 2. Principles of Amnis Imaging Flow Cytometry David A. Basiji 3. Ultrafast Microfluidic Cellular Imaging by Optical Time-Stretch Andy K.S. Lau, Terence T.W. Wong, Ho Cheung Shum, Kenneth K.Y. Wong, and Kevin K. Tsia 4. Applications of Imaging Flow Cytometry for Microalgae Mark Hildebrand, Aubrey Davis, Raffaela Abbriano, Haley R. Pugsley, Jesse C. Traller, Sarah R. Smith, Roshan P. Shrestha, Orna Cook, Eva Sanchez, Kalpana Manandhar-Shrestha, and Benjamin Alderete Part II: IFC in Detection of Nuclei and Nucleic Acids 5. The Analysis of Cell Cycle, Proliferation, and Asymmetric Cell Division by Imaging Flow Cytometry Andrew Filby, William Day, Sukhveer Purewal, and Nuria Martinez-Martin 6. Quantitation of Chromosome Damage by Imaging Flow Cytometry Lindsay A. Beaton-Green and Ruth C. Wilkins 7. Fluorescent In Situ Hybridization In Suspension by Imaging Flow Cytometry Orla Maguire, Paul K. Wallace, and Hans Minderman 8. Analysis of Nucleo-Cytoplasmic Protein Shuttling by Imaging Flow Cytometry Elizaveta Fasler-Kan, Yeldar Baiken, Ivan Vorobjev, and Natasha S. Barteneva 9. Usin g Image-Based Flow Cytometry with a FISH-Based FlowRNA Assay to Simultaneously Detect Intracellular TNF- Protein and mRNA in Monocytes Following LPS Stimulation Adam S. Venable, Andrea L. Henning, Eric A. Prado, and Brian K. McFarlin Part III: IFC in Immune Response and Drug Screening 10. Multiparametric Characterization of Human T-Cell Immune Synapses by InFlow Microscopy Guido H. Wabnitz and Yvonne Samstag 11. Studying T Cells N-Glycosylation by Imaging Flow Cytometry Ana M. Dias, Catarina R. Almeida, Celso A. Reis, and Salome S. Pinho 12. Assessment of Granulocyte Subset Activation: New Information from Image-Based Flow Cytometry Brian K. McFarlin, Eric A. Prado, Adam S. Venable, Andrea L. Henning, and Randall R. Williams 13. Using Image-Based Flow Cytometry to Assess Monocyte Oxidized LDL Phagocytosis Capacity Andrea L. Henning, Adam S. Venable, Eric A. Prado, and Brian K. McFarlin 14. Screening for Drugs Against the Plasmodium falciparum Digestive Vacuole by Imaging Flow Cytometry Yan Quan Lee, Brian E.Hall, and Kevin S.W. Tan Part IV: IFC in Studies of Cell Death and Apoptosis 15. Accurate Assessment of Cell Death by Imaging Flow Cytometry Aja M. Rieger and Daniel R. Barreda 16. Temporal Heterogeneity in Apo ptosis Determined by Imaging Flow Cytometry Ivan A. Vorobjev and Natasha S. Barteneva Part V: Imaging Cytometry in Cell Classification and Morphological Analysis 17. FlowCAM: Quantification and Classification of Phytoplankton by Imaging Flow Cytometry Nicole J. Poulton 18. Detection and Characterization of Rare Circulating Endothelial Cells by Imaging Flow Cytometry Leigh Samsel and J. Philip McCoy, Jr. 19. Imaging Flow Cytometric Analysis of Primary Bone Marrow Megakaryocytes Lisa M. Niswander, James Palis, and Kathleen E. McGrath 20. Sickle Cell Imaging Flow Cytometry Assay (SIFCA) Kleber Y. Fertrin, Leigh Samsel, Eduard J. van Beers, Laurel Mendelsohn, Gregory J. Kato, and J. Philip McCoy

「Nielsen BookData」 より

関連文献： 2件中  1-2を表示

詳細情報

ページトップへ