Cartilage tissue engineering : methods and protocols

著者

    • Doran, Pauline M.

書誌事項

Cartilage tissue engineering : methods and protocols

edited by Pauline M. Doran

（Methods in molecular biology / John M. Walker, series editor, 1340）（Springer protocols）

Humana Press, 2015

大学図書館所蔵 件 / 全2件

この図書・雑誌をさがす

注記

Includes bibliographical references and index

内容説明・目次

内容説明

This volume aims to describe clearly and in detail the key practical skills involved in cartilage tissue engineering. Methods are outlined for isolation and expansion of chondrocytes and stem cells; differentiation; synthesis and application of three-dimensional scaffolds; design and operation of bioreactors; in vivo testing of engineered constructs; and molecular and functional analysis of cartilage cells and tissues. Frequently used technologies are covered, as well as more recent advances in inspirational areas such as 'smart' biomaterial development, novel bioreactor design, -omics analysis, and genetic manipulation of matrix synthesis. The key procedures discussed either underpin the progress already achieved in cartilage tissue engineering or are indicative of the direction of future research in the area. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cartilage Tissue Engineering: Methods and Protocols is a useful resource that informs the scientific community about the experimental work covering a broad range of objectives for cartilage synthesis and regeneration.

目次

Part I: Overview 1. Cartilage Tissue Engineering: What Have We Learned in Practice? Pauline M. Doran Part II: Cell Isolation, Expansion and Differentiation 2. Human Foetal and Adult Chondrocytes Kifah Shahin, Nastaran Mahmoudifar, and Pauline M. Doran 3. Mesenchymal Stem Cells Derived from Human Bone Marrow Oliver F. W. Gardner, Mauro Alini, and Martin J. Stoddart 4. Mesenchymal Stem Cells Derived from Human Adipose Tissue Nastaran Mahmoudifar and Pauline M. Doran 5. Derivation and Chondrogenic Commitment of Human Embryonic Stem Cell-Derived Mesenchymal Progenitors Hicham Drissi, Jason D. Gibson, Rosa Guzzo, and Ren-He Xu 6. Differentiation of Human Induced Pluripotent Stem Cells to Chondrocytes Rosa M. Guzzo and Hicham Drissi 7. Gene Transfer and Gene Silencing in Stem Cells to Promote Chondrogenesis Feng Zhang and Dong-An Wang Part III: Biomaterials and Scaffolds 8. Hydrogels with Tunable Properties Peggy P.Y. Chan 9. Decellularized Extracellular Matrix Scaffols for Cartilage Regeneration Shraddha Thakkar, Hugo Fernandes, and Lorenzo Moroni 10. Use of Interim Scaffolding and Neotissue Development to Produce a Scaffold-Free Living Hyaline Cartilage Graft Ting Ting Lau, Wenyan Leong, Yvonne Peck, Kai Su, and Dong-An Wang 11. Bioprinted Scaffolds for Cartilage Tissue Engineering Hyun-Wook Kang, James J. Yoo, and Anthony Atala 12. Scaffolds for Controlled Release of Cartilage Growth Factors Marie Morille, Marie-Claire Venier-Julienne, and Claudia N. Montero-Menei 13. Nanostructured Capsules for Cartilage Tissue Engineering Clara R. Correia, Rui L. Reis, and Joao F. Mano 14. Stratified Scaffolds for Osteochondral Tissue Engineering Patcharakamon Nooeaid, Gundula Schulze-Tanzil, and Aldo R. Boccaccini Part IV: Bioreactors 15. Mechanobioreactors for Cartilage Tissue Engineering Joanna F. Weber, Roman Perez, and Stephen D. Waldman 16. Shear and Compression Bioreactor for Cartilage Synthesis Kifah Shahin and Pauline M. Doran 17. Microbioreactors for Cartilage Tissue Engineering Yu-Han Chang and Min-Hsien Wu Part V: In Vivo Application 18. Transplantation of Tissue-Engineered Cartilage in an Animal Model (Xenograft and Autograft): Construct Validation Hitoshi Nemoto, Deborah Watson, and Koichi Masuda Part VI: Evaluation 19. Proteomic Analysis of Engineered Cartilage Xinzhu Pu and Julia Thom Oxford 20. Mechanical Testing of Cartilage Constructs Dinorath Olvera, Andrew Daly, and Daniel John Kelly

「Nielsen BookData」 より

関連文献： 2件中  1-2を表示

詳細情報

ページトップへ