Advanced technologies for protein complex production and characterization
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書誌事項
Advanced technologies for protein complex production and characterization
（Advances in experimental medicine and biology, v. 896）
Springer, 2016
- : hbk.
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内容説明・目次
内容説明
This book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists.
Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance.
This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies.
目次
- Preface. Complex Biologics: Engineering the Tools and the Hosts. M. Cristina Vega, Francisco J. Fernandez and Imre Berger Section 1: Introduction 1. Protein complex production from the drug discovery standpoint
- Ismail Moarefi <2. Choose a suitable expression host: a survey of available protein production platforms
- Francisco J. Fernandez and M. Cristina Vega Section 2: Prokaryotic expression hosts 3. ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts
- Yan Nie, Maxime Chaillet, Christian Becke, Matthias Haffke, Martin Pelosse, Daniel Fitzgerald, Ian Collinson, Christiane Schaffitzel and Imre Berger 4. Complex reconstitution and characterization by combining co-expression techniques in Escherichia coli with high-throughput
- Renaud Vincentelli and Christoph Romier 5. Membrane protein production in E. coli for applications in drug discovery
- Harm Jan (Arjan) Snijder and Jonna Hakulinen 6. Cell-free Synthesis of Macromolecular Complexes
- Matthieu Botte, Aurelien Deniaud and Christiane Schaffitzel 7. A Bacillus megaterium system for the production of recombinant proteins and protein complexes
- Rebekka Biedendieck 8. Protein complex production in alternative prokaryotic hosts
- Sara Gomez, Miguel Lopez-Estepa, Francisco J. Fernandez and M. Cristina Vega Section 3: Lower Eukaryotic expression hosts 9. Production of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts
- Francisco J. Fernandez, Miguel Lopez-Estepa, Javier Querol-Garcia and M. Cristina Vega 10. Leishmania tarentolae for the Production of Multi-Subunit Complexes
- Tomoaki Niimi 11. Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes
- Sara Gomez, Miguel Lopez-Estepa, Francisco J. Fernandez, Teresa Suarez and M. Cristina Vega Section 4: Higher Eukaryotic expression hosts 12. Fundamentals of baculovirus expression and applications
- Tom A. Kost and Christopher W. Kemp 13. The MultiBac Baculovirus / Insect Cell Expression Vector System for Producing Complex Protein Biologics
- Duygu Sari, Kapil Gupta, Deepak Balaji Govinda Raj, Alice Aubert, Petra Dmcova, Frederic Garzoni, Daniel Fitzgerald and Imre Berger 14. Fundamentals of expression in mammalian cells
- Michael R. Dyson 15. Assembling multi-subunit complexes using mammalian expression
- Bahar Baser and Joop van den Heuvel Section 5: Plant expression 16. Microalgae as solar-powered protein factories
- Franziska Hempel and Uwe G. Maier 17. Strategies and methodologies for the co-expression of multiple proteins in plants
- Albert Ferrer, Montserrat Arro, David Manzano and Teresa Altabella 18. Transient expression systems in plants-potentialities and constraints
- Tomas Canto Section 6: Complex reconstitution 19. Complex reconstitution from individual protein modules
- Jerome Basquin, Michael Taschner and Esben Lorentzen 20. Structural reconstruction of protein-protein complexes involved in intracellular signaling
- Klara Kirsch, Peter Sok, and Attila Remeny Section 7: Biophysical methods to assess protein complexes 21. The use of small-angle scattering for the characterization of multi subunit complexes
- Adam Round 22. Application of nuclear magnetic resonance and hybrid methods to structure determination of complex systems. Filippo Prischi and Annalisa Pastore
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