Proteomics in biology
著者
書誌事項
Proteomics in biology
(Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 585-586)
Academic Press, 2017
1st ed
- pt. A
- pt. B
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注記
Includes bibliographical references and indexes
内容説明・目次
- 巻冊次
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pt. A ISBN 9780128097427
内容説明
Proteomics in Biology Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field, and a focus on proteomics for this updated volume.
目次
1. An Easy and Fast Protocol for Affinity Bead-Based Protein Enrichment and Storage of Proteome Samples A. Otto, S. Maass, F. Bonn, K. Buttner and D. Becher 2. Filter Aided Sample Preparation the Versatile and Efficient Method for Proteomic Analysis J.R. Wisniewski 3. An Overview of Advanced SILAC Labeling Strategies for Quantitative Proteomics F. Terzi and S. Cambridge 4. Label Free and Standard Free Absolute Quantitative Proteomics Using the 'Total Protein' and 'Proteomic Ruler' Approaches J.R. Wisniewski 5. Separation of Two Distinct O-Glycoforms of Human IgA1 by Serial Lectin-Chromatography Followed by Mass Spectrometry O-Glycan Analysis S. Lehoux and T. Ju 6. Monitoring Protein Synthesis in Caenorhabditis Elegans Using SILAC M. Miyagi 7. Identification of Novel Macropinocytosing Human Antibodies by Phage Display and High-Content Analysis K.D. Ha, S.M. Bidlingmaier, Y. Su, N.-K. Lee and B. Liu 8. Beyond the Natural Proteome: Non-Degenerate Saturation Mutagenesis - Methodologies and Advantages M.M. Ferreira Amaral, L. Frigotto and A.V. Hine 9. WGCNA Application to Proteomic and Metabolomic Data Analysis G. Pei, L. Chen and W. Zhang 10. A Protocol for Large-Scale Proteomic Analysis of Microdissected Formalin Fixed and Paraffin Embedded Tissue P. Ostasiewicz and J.R. Wisniewski 11. Body Fluid Degradomics and Characterization of Basic N-Terminome F. Sabino, O. Hermes and U. auf dem Keller 12. Reannotation of Genomes by Means of Proteomics Data J. Armengaud 13. Proteogenomics: Recycling Public Data to Improve Genome Annotations A. McAfee and L.J. Foster 14. Targeted in-Depth Quantification of Signaling Using Label-Free Mass Spectrometry P.R. Cutillas 15. Biotin Switch Assays for Quantitation of Reversible Cysteine Oxidation R. Li and J. Kast 16. Application of a Thioredoxin Trapping Mutant for Analysis of the Cellular Nitrosoproteome M. Benhar 17. Combining Click-Chemistry Based Proteomics with Dox-Inducible Gene Expression J. Gebert, M. Schnoelzer, U. Warnken and J. Kopitz 18. Analysis of the Proteome of Hair-Cell Stereocilia by Mass Spectrometry J.F. Krey, P.A. Wilmarth, L.L. David and P.G. Barr-Gillespie 19. Proteomic Analysis of Metabolic Responses to Biofuels and Chemicals in Photosynthetic Cyanobacteria T. Sun, L. Chen and W. Zhang 20. Deep Profiling of Proteome and Phosphoproteome by Isobaric Labeling, Extensive Liquid Chromatography and Mass Spectrometry B. Bai, H. Tan, V.R. Pagala, A.A. High, V.P. Ichhaporia, L. Hendershot and J. Peng 21. Glycoprotein Enrichment Analytical Techniques: Advantages and Disadvantages R. Zhu, L. Zacharias, K.M. Wooding, W. Peng and Y. Mechref 22. Quantitative Glycomics: A Combined Analytical and Bioinformatics Approach L. Veillon, S. Zhou and Y. Mechref
- 巻冊次
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pt. B ISBN 9780128097434
内容説明
Proteomics in Biology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in proteomics.
目次
1. Protein Cysteinyl-S-Nitrosylation: Analysis and Quantification 2. Quantitative Proteomics of the E. coli Membranome 3. Comparative Analysis and Validation of Different Steps in Glycomics Studies 4. Analysis of Translocation-Competent Secretory Proteins by HDX-MS 5. Site-Specific Quantification of Lysine Acetylation Using Isotopic Labeling 6. Posttranslational Modifications and Plant-Environment Interaction 7. Identification of Posttranslational Modifications of Endogenous Chromatin Proteins From Testicular Cells by Mass Spectrometry 8. Quantitative Analysis of Yeast Checkpoint Protein Kinase Activity by Combined Mass Spectrometry Enzyme Assays 9. Retrieving Quantitative Information of Histone PTMs by Mass Spectrometry 10. Evaluating Exosome Protein Content Changes Induced by Virus Activity Using SILAC Labeling and LC-MS/MS 11. Exoproteomics of Pathogens: Analysis of Toxins and Other Virulence Factors by Proteomics 12. Integrated and Quantitative Proteomics of Human Tumors 13 .Mass Spectrometry-Based Analysis for the Discovery and Validation of Potential Colorectal Cancer Stool Biomarkers 14. Mass Spectrometry-Based Methodology for Identification of Native Histone Variant Modifications From Mammalian Tissues and Solid Tumors 15. Drug Target Identification Using an iTRAQ-Based Quantitative Chemical Proteomics Approach-Based on a Target Profiling Study of Andrographolide 16. A Super-SILAC Strategy for the Accurate and Multiplexed Profiling of Histone Posttranslational Modifications 17. A Comparison of Two-Hybrid Approaches for Detecting Protein-Protein Interactions 18. Recent Achievements in Characterizing the Histone Code and Approaches to Integrating Epigenomics and Systems Biology 19. Rapid Proteomics to Prospect and Validate Novel Bacterial Metabolism Induced by Environmental Burden 20. Quantitation of Human Metallothionein Isoforms in Cells, Tissues, and Cerebrospinal Fluid by Mass Spectrometry 21. A Cautionary Tale on the Inclusion of Variable Posttranslational Modifications in Database-Dependent Searches of Mass Spectrometry Data 22. Kinase Assay-Linked Phosphoproteomics: Discovery of Direct Kinase Substrates
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