RNA-protein complexes and interactions : methods and protocols
著者
書誌事項
RNA-protein complexes and interactions : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1421）（Springer protocols）
Humana Press : Springer, c2016
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This detailed volume explores the continuing techniques of
studying RNA-protein complexes and interactions as research in these areas
expand. After an introductory chapter,
the book continues with ways to purify RNA-protein complexes assembled in cells
or in isolated cellular extracts, methods for measuring various biochemical
activities of RNA-interacting proteins or ribonucleoproteins, biochemical
methods for measuring direct RNA-protein contact, as well as various new or
innovative methods pertinent to the subject. Written for the highly successful Methods in Molecular Biology series,
chapters contain brief introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, RNA-Protein
Complexes and Interactions: Methods and Protocols provides a set of useful
protocols, both basic and advanced, designed to inspire researchers working
with RNA and RNA-interacting proteins.
目次
1. Characterization of RNA-Protein
Interactions: Lessons from Two RNA-Binding Proteins, SRSF1 and SRSF2
Lindsey
Skrdlant and Ren-Jang Lin
2. Identification of mRNA-Interacting Factors by
MS2-TRAP (MS2-Tagged RNA Affinity Purification)
Je-Hyun Yoon and Myriam Gorospe
3. Biotin-Streptavidin Affinity Purification of
RNA-Protein Complexes Assembled In Vitro
Shuai Hou, Lei Shi, and
Haixin Lei
4. Detecting RNA-Protein Interaction Using End-Labeled Biotinylated
RNA Oligonucleotides and Immunoblotting
Xuexiu Zheng, Sunghee Cho, Heegyum
Moon, Tiing Jen Loh, Hana Jang, and Haihong Shen
5. Purification of RNA-Protein Splicing Complexes Using a
Tagged Protein from In Vitro Splicing Reaction Mixture
Naoyuki Kataoka
6. Loading of Argonaute Protein with Small
Duplex RNA in Cellular Extracts
Keith T. Gagnon
7. In Vitro Analysis of Ribonucleoprotein Complex
Remodeling and Disassembly
Hua-Lin Zhou and Hua Lou
8. Single-Turnover Kinetics of Methyl
Transfer to tRNA by Methyltransferases
Ya-Ming Hou
9. Purification and Functional Reconstitution of Box
H/ACA Ribonucleoprotein Particles
Chao Huang, Guowei Wu, and Yi-Tao Yu
10. Northwestern Blot Analysis: Detecting RNA-Protein
Interaction After Gel Separation of Protein Mixture
Shangbing Zang and Ren-Jang Lin
11. Measuring mRNA Translation by Polysome Profiling
Marek Kudla and Fedor V. Karginov
12. Genome-Wide Profiling of RNA-Protein
Interactions Using CLIP-Seq
Cheryl Stork and Sika Zheng
13. Identification of Endogenous mRNA-Binding Proteins in
Yeast Using Crosslinking and PolyA Enrichment
Sarah F. Mitchell and Roy Parker
14. Ribo-Proteomics Approach to Profile RNA-Protein and
Protein-Protein Interaction Networks
Hsin-Sung Yeh, Jae-Woong Chang, and
Jeongsik Yong
15. Detection of Protein-Protein Interaction Within an
RNA-Protein Complex Via Unnatural-Amino-Acid-Mediated Photochemical
Crosslinking
Fu-Lung Yeh, Luh Tung,
and Tien-Hsien Chang
16. Evolution of Cell-Type-Specific RNA
Aptamers Via Live Cell-Based SELEX
Jiehua Zhou and John J. Rossi
17. mCarts: Genome-Wide Prediction of Clustered Sequence
Motifs as Binding Sites for RNA Binding Proteins
Sebastien M. Weyn-Vanhentenryck and
Chaolin Zhang
18. Design of RNA-Binding Proteins:
Manipulate Alternative Splicing in Human Cells with Artificial Splicing Factors
Yang Wang and Zefeng Wang
19. Informational Suppression to Probe RNA:RNA
Interactions in the Context of Ribonucleoproteins: U1 and 5' Splice-Site
Base-Pairing
Jiazi Tan and Xavier Roca
20. Analysis of Alternative Pre-RNA Splicing in the Mouse
Retina Using a Fluorescent Reporter
Daniel Murphy, Saravanan
Kolandaivelu, Visvanathan Ramamurthy, and Peter Stoilov
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