Plant cytogenetics : methods and protocols
Author(s)
Bibliographic Information
Plant cytogenetics : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1429）（Springer protocols）
Humana Press : Springer, c2016
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Description and Table of Contents
Description
This volume covers a
range of methods used in plant cytogenetics, beginning with basic analysis of
chromosomes and visualizing gene locations, to manipulating and dissecting
chromosomes, and then focusing on less understood features of chromosomes such
as recombination initiation sites and epigenomic marks. The methods described
in Plant Cytogenetics: Methods and Protocols build on each other and
provide, those new to the field, with a comprehensive platform to support their
research endeavours, while also introducing advanced techniques to experienced
researchers. Written in the highly successful Methods in Molecular Biology series
format, chapters include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting
edge and thorough, Plant Cytogenetics: Methods and Protocols, is a
valuable resource for anyone who is interested in the diverse and wonderfully
complex field of cytogenetics.
Table of Contents
1. C-Banding of Plant Chromosomes
Eric N. Jellen
2. Chromosome Painting by GISH and Multi-Color
FISH
Steven S. Xu, Zhao Liu, Qijun Zhang,
Zhixia Niu, Chao-Chien Jan, and Xiwen Cai
3. Fluorescent In Situ Hybridization on Extended Chromatin Fibers for
High-Resolution Analysis of Plant Chromosomes
Daryna Dechyeva and Thomas Schmidt
4. Tyramide Signal Amplification: Fluorescence
In Situ Hybridization for Identifying
Homologous Chromosomes
Araceli Fominaya, Yolanda Loarce,
Juan M. Gonzalez, and Esther Ferrer
5. Localization
of Low-Copy DNA Sequences on Mitotic Chromosomes by FISH
Miroslava Karafiatova, Jan Bartos,
Jaroslav Dolezel
6. Immunolabelling
and In Situ Labelling of Isolated
Plant Interphase Nuclei
Ali Pendle and Peter Shaw
7. Manipulation
of Homologous Chromosome Recombination in Wheat
Adam J. Lukaszewski
8. Dissecting
Plant Chromosomes by the Use of Ionizing Radiation
Penny M.A. Kianian, Katie L.
Liberatore, Marisa Miller, Justin B. Hegstad, and Shahryar F. Kianian
9. Optical
Nano-Mapping and Analysis of Plant Genomes
Ming-Cheng Luo, Karin R. Deal, Armond
Murray, Tingting Zhu, Alex R. Hastie, Will Stedman, Henry Sadowski, and Michael
Saghbini
10. Flow
Sorting Plant Chromosomes
Jan Vrana, Petr Capal,
Jarmila Cihalikova, Marie Kubalakova, and Jaroslav Dolezel
11. Construction
of BAC Libraries from Flow-Sorted Chromosomes
Jan Safar, Hana Simkova, and Jaroslav Dolezel
12. The
Chromosome Microdissection and Microcloning Technique
Ying-Xin Zhang, Chuan-Liang Deng, and
Zan-Min Hu
13. Immunolocalization
on Whole Anther Chromosome Spreads for Male Meiosis
Stefanie Dukowic-Schulze, Anthony
Harris, and Changbin Chen
14. Mapping
Recombination Initiation Sites Using Chromatin Immunoprecipitation
Yan He, Minghui Wang, Qi Sun, and
Wojciech P. Pawlowski
15. Chromatin
Immunoprecipitation to Study Plant Epigenome
Zidian Xie and Gernot Presting
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