Post-transcriptional gene regulation
著者
書誌事項
Post-transcriptional gene regulation
（Methods in molecular biology / John M. Walker, series editor, v. 1358）
Humana, c2016
2nd ed.
大学図書館所蔵 全1件
注記
Includes bibliographical references and index
内容説明・目次
内容説明
This volume aims to provide the most recent advances in techniques for studying gene expression regulation at the post-transcriptional level. Post-Transcriptional Gene Regulation, Second Edition is organized in six sections describing bioinformatics approaches for studying post-transcriptional regulation, various expression profiling approaches, protein-RNA interaction and non-coding RNA investigation techniques, methods for profiling RNA modifications, and other techniques such as alternative translation initiation or polyadenylation sites determination. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Post-Transcriptional Gene Regulation, Second Edition is a versatile resource to researchers studying post-transcriptional regulation, introducing the most recent techniques and providing a comprehensive guide to their implementation.
目次
PART I: Bioinformatics
1. Introduction to Bioinformatics Resources for Post-Transcriptional Regulation of Gene Expression
Alessandro Quattrone and Erik Dassi
2. A Computational Approach for the Discovery of Protein-RNA Networks
Domenica Marchese, Carmen Maria Livi, and Gian Gaetano Tartaglia
PART II: Expression Studies
3. Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian Cells
Hannah Pham, Nicola A. Kearns, and Rene Maehr
4. Studying the Translatome with Polysome Profiling
Paola Zuccotti and Angelika Modelska
5. Exploring Ribosome Positioning on Translating Transcripts with Ribosome Profiling
Pieter Spealman, Hao Wang, Gemma May, Carl Kingsford, and C. Joel Mcmanus
6. Studying Isoform-Specific Recruitment to Polyribosomes with Frac-Seq
Rocio T. Martinez-Nunez and Jeremy Sanford
7. Use of the Pbutr Reporter System for Scalable Analysis of 3' UTR-Mediated Gene Regulation
Arindam Chaudhury and Joel R. Neilson
PART III: RBP Interactomics
8. Comprehensive Identification of RNA-Binding Proteins by RNA Interactome Capture
Alfredo Castello, Rastislav Horos, Claudia Strein, Bernd Fischer, Katrin Eichelbaum, Lars M. Steinmetz, Jeroen Krijgsveld, and Matthias W. Hentze
9. Identifying RBP Targets with RIP-Seq
Hans-Herman Wessels, Antje Hirsekorn, Uwe Ohler, and Neelanjan Mukherjee
10. PAR-CLIP: A Method for Transcriptome-Wide Identification of RNA Binding Protein Interaction Sites
Charles Danan, Sudhir Manickavel, and Markus Hafner
11. Profiling the Binding Sites of RNA-Binding Proteins with Nucleotide Resolution Using Iclip
FX Reymond Sutandy, Andrea Hildebrandt, and Julian Koenig
12. A Pipeline for PAR-CLIP Data Analysis
Marvin Jens
PART IV: Non-Coding RNAs
13. Capture and Identification Of Mirna Targets By Biotin Pulldown And RNA-Seq
Shen Mynn Tan and Judy Lieberman
14. Identification of Mirna-Target RNA Interactions Using CLASH
Alexandra Helwak and David Tollervey
PART V: RNA Modifications
15. Genome-Wide Analysis of A-To-I RNA Editing
Yiannis A. Savva, Georges St. Laurent, and Robert A. Reenan
16. Nucleotide-Level Profiling Of M5c RNA Methylation
Tennille Sibbritt, Andrew Shafik, Susan J. Clark, and Thomas Preiss
17. Probing N6-Methyladenosine (M6a) RNA Modification in Total RNA with SCARLET
Nian Liu and Tao Pan
PART VI: Other Aspects Of PTR
18. Genome-Wide Identification of Alternative Polyadenylation Events Using 3'T-Fill
Stefan Wilkening, Vicent Pelechano, and Lars M. Steinmetz
19. Genome-Wide Profiling Of Alternative Translation Initiation Sites
Xiangwei Gao, Ji Wan, and Shu-Bing Qian
20. Genome-Wide Study Of Mrna Isoform Half-Lives
Joseph V. Geisberg and Zarmik Moqtaderi
21. Visualizing Mrna Dynamics in Live Neurons and Brain Tissues
Hye Yoon Park and Minho Song
22. Single Molecule Live-Cell Visualization of Pre-Mrna Splicing
Robert M. Martin, Jose Rino, Ana C. De Jesus, and Maria Carmo-Fonseca
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