Post-transcriptional gene regulation

著者

    • Dassi, Erik

書誌事項

Post-transcriptional gene regulation

edited by Erik Dassi

（Methods in molecular biology / John M. Walker, series editor, v. 1358）

Humana, c2016

2nd ed.

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This volume aims to provide the most recent advances in techniques for studying gene expression regulation at the post-transcriptional level. Post-Transcriptional Gene Regulation, Second Edition is organized in six sections describing bioinformatics approaches for studying post-transcriptional regulation, various expression profiling approaches, protein-RNA interaction and non-coding RNA investigation techniques, methods for profiling RNA modifications, and other techniques such as alternative translation initiation or polyadenylation sites determination. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Post-Transcriptional Gene Regulation, Second Edition is a versatile resource to researchers studying post-transcriptional regulation, introducing the most recent techniques and providing a comprehensive guide to their implementation.

目次

PART I: Bioinformatics 1. Introduction to Bioinformatics Resources for Post-Transcriptional Regulation of Gene Expression Alessandro Quattrone and Erik Dassi 2. A Computational Approach for the Discovery of Protein-RNA Networks Domenica Marchese, Carmen Maria Livi, and Gian Gaetano Tartaglia PART II: Expression Studies 3. Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian Cells Hannah Pham, Nicola A. Kearns, and Rene Maehr 4. Studying the Translatome with Polysome Profiling Paola Zuccotti and Angelika Modelska 5. Exploring Ribosome Positioning on Translating Transcripts with Ribosome Profiling Pieter Spealman, Hao Wang, Gemma May, Carl Kingsford, and C. Joel Mcmanus 6. Studying Isoform-Specific Recruitment to Polyribosomes with Frac-Seq Rocio T. Martinez-Nunez and Jeremy Sanford 7. Use of the Pbutr Reporter System for Scalable Analysis of 3' UTR-Mediated Gene Regulation Arindam Chaudhury and Joel R. Neilson PART III: RBP Interactomics 8. Comprehensive Identification of RNA-Binding Proteins by RNA Interactome Capture Alfredo Castello, Rastislav Horos, Claudia Strein, Bernd Fischer, Katrin Eichelbaum, Lars M. Steinmetz, Jeroen Krijgsveld, and Matthias W. Hentze 9. Identifying RBP Targets with RIP-Seq Hans-Herman Wessels, Antje Hirsekorn, Uwe Ohler, and Neelanjan Mukherjee 10. PAR-CLIP: A Method for Transcriptome-Wide Identification of RNA Binding Protein Interaction Sites Charles Danan, Sudhir Manickavel, and Markus Hafner 11. Profiling the Binding Sites of RNA-Binding Proteins with Nucleotide Resolution Using Iclip FX Reymond Sutandy, Andrea Hildebrandt, and Julian Koenig 12. A Pipeline for PAR-CLIP Data Analysis Marvin Jens PART IV: Non-Coding RNAs 13. Capture and Identification Of Mirna Targets By Biotin Pulldown And RNA-Seq Shen Mynn Tan and Judy Lieberman 14. Identification of Mirna-Target RNA Interactions Using CLASH Alexandra Helwak and David Tollervey PART V: RNA Modifications 15. Genome-Wide Analysis of A-To-I RNA Editing Yiannis A. Savva, Georges St. Laurent, and Robert A. Reenan 16. Nucleotide-Level Profiling Of M5c RNA Methylation Tennille Sibbritt, Andrew Shafik, Susan J. Clark, and Thomas Preiss 17. Probing N6-Methyladenosine (M6a) RNA Modification in Total RNA with SCARLET Nian Liu and Tao Pan PART VI: Other Aspects Of PTR 18. Genome-Wide Identification of Alternative Polyadenylation Events Using 3'T-Fill Stefan Wilkening, Vicent Pelechano, and Lars M. Steinmetz 19. Genome-Wide Profiling Of Alternative Translation Initiation Sites Xiangwei Gao, Ji Wan, and Shu-Bing Qian 20. Genome-Wide Study Of Mrna Isoform Half-Lives Joseph V. Geisberg and Zarmik Moqtaderi 21. Visualizing Mrna Dynamics in Live Neurons and Brain Tissues Hye Yoon Park and Minho Song 22. Single Molecule Live-Cell Visualization of Pre-Mrna Splicing Robert M. Martin, Jose Rino, Ana C. De Jesus, and Maria Carmo-Fonseca

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