Cohesin and condensin : methods and protocols
著者
書誌事項
Cohesin and condensin : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1515）（Springer protocols）
Humana Press, c2017
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
The volume provides comprehensive, state-of-the-art experimental techniques that are now available to dissect the molecular mechanisms of regulation and function of cohesin and the related factor condensin in vitro and in vivo across different model organisms, as well as in human cells. Cohesin and Condensin: Methods and Protocols is divided into three parts: Part I explores various in vitro and in vivo systems used to study the fundamental mechanism of cohesin regulation in mitosis and meiosis; Part II summarizes experimental systems in a variety of organisms that are used to address interphase functions of cohesin and Nipbl in gene regulation and chromatin interaction, ribosome biogenesis and DNA repair, which contribute significantly to cohesion-associated disorders; Part III covers related condensin complex and describes techniques to study its role in mitosis and interphase. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Cohesin and Condensin: Methods and Protocols is a valuable resource for diverse audiences with interests in the relationship between chromatin organization and genomic functions.
目次
Part I: In Vitro and In Vivo Systems to Dissect Molecular Mechanisms of Cohesin Regulation in Mitosis and Meiosis
1. A Sister Chromatid Cohesion Assay Using Xenopus Egg Extracts
Keishi Shintomi and Tatsuya Hirano
2. An In Vitro Assay for Monitoring Topological DNA Entrapment by the Chromosomal Cohesin Complex
Yasuto Murayama and Frank Uhlmann
3. Biochemical and Functional Assays of Human Cohesin-Releasing Factor Wapl
Ge Zheng, Zhuqing Ouyang, and Hongtao Yu
4. Detection of Cohesin SUMOylation In Vivo
Marcelino Bermudez-Lopez and Luis Aragon
5. Analysis of Meiotic Sister Chromatid Cohesion in Caenorhabditis elegans
Aaron F. Severson
6. Protein and Chromosome Analysis in Mammalian Meiocytes
Michelle Stevense, Rolf Jessberger, and Attila Toth
Part II: The Roles of Cohesin in Gene Regulation, Chromatin Interactions, Ribosome Biogenesis, and DNA Repair
7. Resolving the Genomic Localization of the Kollerin Cohesin-Loader Complex
Kerstin S. Wendt
8. Measuring Sister Chromatid Cohesion Protein Genome Occupancy in Drosophila melanogaster by ChIP-seq
Dale Dorsett and Ziva Misulovin
9. A Dual-Color Reporter Assay of Cohesin-Mediated Gene Regulation in Budding Yeast Meiosis
Jinbo Fan, Hui Jin, and Hong-Guo Yu
10. Methods to Study the Atypical Roles of DNA Repair and SMC Proteins in Gene Silencing
Misty R. Peterson, Omar Hamdani, and Rohinton T. Kamakaka
11. Zebrafish as a Model to Study Cohesin and Cohesinopathies
Akihiko Muto and Thomas F. Schilling
12 Analysis of Cohesin Function in Gene Regulation and Chromatin Organization in Interphase
Preksha Gupta, Thais Lavagnolli, Hegias Mira-Bontenbal, and Matthias Merkenschlager
13. Using Fluorescent Reporters in Conjunction with Cytometry and Statistics to Assess Nuclear Accumulation of Ribosomal Proteins
Dong-Hwan Kim, Andrew C. Box, Hua Li, and Jennifer L. Gerton
14. The Use of Laser Microirradiation to Investigate the Roles of Cohesins in DNA Repair
Xiangduo Kong, Alexander R. Ball, Jr., and Kyoko Yokomori
Part III: Dissection of Condensin Function in Mitotic Chromosome Condensation and Gene Regulation
15. A Protocol for Measuring Mitotic Chromosome Condensation Quantitatively in Fission Yeast CellsChristoph Schiklenk, Boryana Petrova, and Christian H. Haering
16. ChIP-seq Analysis of Condensin Complex in Cultured Mammalian Cells
Toyonori Sakata, Katsuhiko Shirahige, Takashi Sutani
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