Biomedical nanotechnology : methods and protocols

著者

    • Petrosko, Sarah Hurst
    • Day, Emily S.

書誌事項

Biomedical nanotechnology : methods and protocols

edited by Sarah Hurst Petrosko, Emily S. Day

(Methods in molecular biology / John M. Walker, series editor, 1570)(Springer protocols)

Humana Press, c2017

2nd ed.

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This second edition volume provides an overview of some of the types of nanostructures commonly used in nanobiomedicine. The chapters in this book discuss practical information on the synthesis and characterization of a variety of solution-phase and surface-bound nanomaterials, with examples of how they can be used in sensing, imaging, and therapeutics. Specific topics include the synthesis and characterization of molecule and biomolecule-functionalized nanoconjugates with gold, iron oxide, or polymeric cores; the development of biosensing, imaging, and therapeutic applications of multicomponent/multifunctional nanostructures; and the application of flow cytometry in nanobiomedicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.< Thorough and comprehensive, Biomedical Nanotechnology: Methods and Protocols, Second Edition is a useful resource for scientists and researchers at all levels who are interested in working in a new area of nanoscience and technology, or in expanding their knowledge base in their current field.

目次

Preface... Table of Contents... Contributing Authors... 1. Quantification of siRNA Duplexes Bound to Gold Nanoparticle Surfaces Jilian R. Melamed, Rachel S. Riley, Danielle M. Valcourt, Margaret M. Billingsley, Nicole L. Kreuzberger, and Emily S. Day 2. Ligand Exchange and 1H NMR Quantification of Single- and Mixed-Moiety Thiolated Ligand Shells on Gold Nanoparticles Ashley M. Smith and Jill E. Millstone 3. Nanoparticle Tracking Analysis for Determination of Hydrodynamic Diameter, Concentration, and Zeta Potential of Polyplex Nanoparticles David R. Wilson and Jordan J. Green 4. Magnetic Characterization of Iron Oxide Nanoparticles for Biomedical Applications Lorena Maldonado-Camargo, Mythreyi Unni, and Carlos Rinaldi 5. Preparation of Magnetic Nanoparticles for Biomedical Applications Xiaolian Sun and Shouheng Sun 6. Brain-Penetrating Nanoparticles for Analysis of the Brain Microenvironment Elizabeth Nance 7. Volumetric Bar-Chart Chips for Biosensing Yujun Song, Ying Li, and Lidong Qin 8. qFlow Cytometry-Based Receptoromic Screening: A High-Throughput Quantification Approach Informing Biomarker Selection and Nanosensor Development Si Chen, Jared Weddell, Pavan Gupta, Grace Conard, James Parkin, and Princess I. Imoukhuede 9. Evaluating Nanoparticle Binding to Blood Compartment Immune Cells in High-Throughput with Flow Cytometry Shann S. Yu 10. A Gold@Polydopamine Core-Shell Nanoprobe for Long-Term Intracellular Detection of MicroRNAs in Differentiating Stem Cells Chun Kit K. Choi, Chung Hang J. Choi, and Liming Bian 11. Antibody-Conjugated Single Quantum Dot Tracking of Membrane Neurotransmitter Transporters in Primary Neuronal Cultures Danielle M. Bailey, Oleg Kovtuna, and Sandra J. Rosenthal 12. Spectroscopic Photoacoustic Imaging of Gold Nanorods Austin Van Namen and Geoffrey P. Luke 13. Dual Wavelength-Triggered Gold Nanorods for Anti-Cancer Treatment Dennis B. Pacardo, Frances S. Ligler, and Zhen Gu 14. Photolabile Self-Immolative DNA-Drug Nanostructures Xuyu Tan and Ke Zhang 15. Enzyme-Responsive Nanoparticles for the Treatment of Disease Cassandra E. Callmann and Nathan C. Gianneschi 16. NanoScript: A Versatile Nanoparticle-Based Synthetic Transcription Factor for Innovative Gene Manipulation Kholud Dardir, Christopher Rathnam, and KiBum Lee 17. Glucose-Responsive Insulin Delivery by Microneedle-Array Patches Loaded with Hypoxia-Sensitive Vesicles Jicheng Yu, Yuqi Zhang, and Zhen Gu 18. Electrospun Nanofiber Scaffolds and their Hydrogel Composites for the Engineering and Regeneration of Soft Tissues Ohan S. Manoukian, Rita Matta, Justin Letendre, Paige Collins, Augustus D. Mazzocca, and Sangamesh G. Kumbar 19. Application of Hydrogel Template Strategy in Ocular Drug Delivery Crystal S. Shin, Daniela C. Marcano, Kinam Park, and Ghanashyam Acharya 20. High-Accuracy Determination of Cytotoxic Responses from Graphene Oxide Exposure using Imaging Flow Cytometry Sandra Vranic and Kostas Kostarelos 21. Air-Liquid Interface Cell Exposures to Nanoparticle Aerosols Nastassja A. Lewinski, Nathan J. Liu, Akrivi Asimakopoulou, Eleni Papaioannou, Athanasios Konstandopoulos, and Michael Riediker 22. Returning to the Patent Landscapes for Nanotechnology: Assessing the Garden That It Has Grown Into Diana M. Bowman, Douglas J. Sylvester, and Anthony D. Marino

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