Synthetic Protein Switches : methods and protocols
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Bibliographic Information
Synthetic Protein Switches : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1596）（Springer protocols）
Humana Press, c2017
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Includes bibliographical references and index
Description and Table of Contents
Description
This detailed book provides state-of-the-art protocols to facilitate the construction of synthetic protein switches for a variety of applications in biotechnology and basic research. Beginning with general strategies, the volume continues with coverage of peptide switches, fluorescent and bioluminescent sensors, -lactamase sensors, proteolytic sensors, optogenetic switches, as well as cellular signaling switches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Synthetic Protein Switches: Methods and Protocols serves as a useful reference for life scientists from diverse research fields that range from traditional, discovery-centered disciplines, like cancer research, to newly emerging disciplines, such as synthetic biology.
Table of Contents
Part I: General Strategies and Considerations
1. Synthetic Protein Switches: Theoretical and Experimental Considerations
Viktor Stein
2. Construction of Allosteric Protein Switches by Alternate Frame Folding and Intermolecular Fragment Exchange
Jeung-Hoi Ha and Stewart N. Loh
3. Construction of Protein Switches by Domain Insertion and Directed Evolution
Lucas F. Ribeiro, Tiana D. Warren, and Marc Ostermeier
Part II: Peptide Switches
4. Catalytic Amyloid Fibrils that Bind Copper to Activate Oxygen
Alex Sternisha and Olga Makhlynets
Part III: Fluorescent and Bioluminescent Sensors
5. Ancestral Protein Reconstruction and Circular Permutation for Improving the Stability and Dynamic Range of FRET Sensors
Ben E. Clifton, Jason H. Whitfield, Inmaculada Sanchez-Romero, Michel K. Herde, Christian Henneberger, Harald Janovjak, and Colin J. Jackson
6. Method for Developing Optical Sensors Using a Synthetic Dye-Fluorescent Protein FRET Pair and Computational Modelling and Assessment
Joshua A. Mitchell, William H. Zhang, Michel K. Herde, Christian Henneberger, Harald Janovjak, Megan L. O'Mara, and Colin J. Jackson
7. Rational Design and Applications of Semi-Synthetic Modular Biosensors: SNIFITs and LUCIDs
Helen Farrants, Julien Hiblot, Rudolf Griss, and Kai Johnsson
8. Ultrasensitive Firefly Luminescent Intermediate-Based Protein-Protein Interaction Assay (FlimPIA) Based on the Functional Complementation of Mutant Firefly Luciferases
Yuki Ohmuro-Matsuyama and Hiroshi Ueda
9. Quantitative and Dynamic Imaging of ATM Kinase Activity
Shyam Nyati, Grant Young, Brian Dale Ross, and Alnawaz Rehemtulla
Part IV: -lactamase Sensors
10. Creation of Antigen-Dependent -Lactamase Fusion Protein Tethered by Circularly Permuted Antibody Variable Domains
Hiroto Iwai, Miki Kojima-Misaizu, Jinhua Dong, and Hiroshi Ueda
11. Protein and Protease Sensing by Allosteric Derepression
Goh Hui Chin, Farid J. Ghadessy, and Saurabh Nirantar
12. DNA-Specific Biosensors Based on Intramolecular -Lactamase-Inhibitor Complex Formation
Wouter Engelen and Maarten Merkx
Part V: Proteolytic Sensors
13. Engineering and Characterizing Synthetic Protease Sensors and Switches
Viktor Stein and Kirill Alexandrov
14. Characterizing Dynamic Protein-Protein Interactions Using the Genetically Encoded Split Biosensor Assay Technique Split TEV
Jan P. Wintgens, Moritz J Rossner, and Michael C. Wehr
Part VI: Optogenetic Switches
15. Development of a Synthetic Switch to Control Protein Stability in Eukaryotic Cells with Light
Christof Taxis
16. Light-Regulated Protein Kinases Based on the CRY2-CIB1 System
Wignand W.D. Muhlhauser, Maximilian Hoerner, Wilfried Weber, and Gerald Radziwill
17. Yeast-Based Screening System for the Selection of Functional Light-Driven K+ Channels
Cristian Cosentino, Laura Alberio, Gerhard Thiel, and Anna Moroni
18. Primer-Aided Truncation for the Creation of Hybrid Proteins
Robert Stabel, Birthe Stuven, Robert Ohlendorf, and Andreas Moeglich
Part VII: Cellular Signaling Switches
19. Engineering Small Molecule Responsive Split-Protein Kinases
Javier Castillo-Montoya and Indraneel Ghosh
20. Directed Evolution Methods to Rewire Signaling Networks
Raphael B. Di Roberto, Benjamin M. Scott, and Sergio G. Peisajovich
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