Plant genomics : methods and protocols

著者

• Busch, Wolfgang

書誌事項

Plant genomics : methods and protocols

edited by Wolfgang Busch

（Methods in molecular biology / John M. Walker, series editor, 1610）（Springer protocols）

Humana Press : Springer Nature, c2017

• : hard

注記

"This Humana Press imprint is published by Springer Nature. The registered company is Springer Science+Business Media LLC"--T.p. verso

Includes bibliographical references and index

内容説明・目次

内容説明

This volume provides protocols that revolve around three pillars of progress in the plant genomics field: genotypes, phenotypes, and the molecular processes in between. Chapters in Plant Genomics: Methods and Protocols are not restricted to the predominant model species Arabidopsis thaliana, hoping to encourage and facilitate other researchers to expand their research to other species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Genomics: Methods and Protocols aims to serve as an inspiration for further studies in plant genomics.

目次

Part I: Genotypes 1. CRISPR/Cas-mediated in planta Gene Targeting Simon Schiml, Friedrich Fauser, and Holger Puchta 2. User Guide for the LORE1 Insertion Mutant Resource Terry Mun, Anna Malolepszy, Niels Sandal, Jens Stougaard, and Stig U. Andersen 3. Enabling Reverse Genetics in Medicago truncatula Using High Throughput Sequencing for Tnt1 Flanking Sequence Recovery Xiaofei Cheng, Nick Krom, Shulan Zhang, Kirankumar S. Mysore, Michael Udvardi, and Jiangqi Wen 4. Generation of Doubled Haploid Lines for QTL Mapping Daniele L. Filiault, Danelle K. Seymour, Ravi Maruthachalam, and Julin N. Maloof Part II: Phenotypes 5. Assessing Distribution and Variation of Genome-wide DNA Methylation using Short Read Sequencing Joerg Hagmann and Claude Becker 6. Circular Chromosome Conformation Capture in Plants Stefan Grob 7. Genome Wide Profiling of Histone Modifications and Histone Variants in Arabidopsis thaliana and Marchantia polymorpha Ramesh Yelagandula, Akihisa Osakabe, Elin Axelsson, Frederic Berger, and Tomokazu Kawashima 8. Tissue-Specific Transcriptome Profiling in Arabidopsis Roots Erin E. Sparks and Philip N. Benfey 9. Sample Preparation Protocols for Protein Abundance, Acetylome and Phosphoproteome Profiling of Plant Tissues Gaoyuan Song, Maxwell R McReynolds, and Justin W Walley 10. Automated High-throughput Root Phenotyping of Arabidopsis thaliana under Nutrient Deficiency Conditions Santosh B. Satbhai, Christian Go schl, and Wolfgang Busch< 11. Large-scale Phenotyping of Root Traits in the Model Legume Lotus japonicus Marco Giovannetti, Anna Malolepszy, Christian Goeschl, and Wolfgang Busch 12. Long-term Confocal Imaging of Arabidopsis thaliana Roots for Simultaneous Quantification of Root Growth and Fluorescent Signals Delyana Stoeva, Christian Goeschl, and Wolfgang Busch Part III: Molecular Bases of Phenotypes 13. Identification of Protein-DNA Interactions Using Enhanced Yeast One-Hybrid Assays and a Semi-Automated Approach Allison Gaudinier, Michelle Tang, Anne-Maarit Bagman, and Siobhan M. Brady 14. Mapping Protein- protein Interaction using High-throughput Yeast-2-Hybrid Jessica Lopez and M. Shahid Mukhtar 15. Mapping Protein-protein Interactions using Affinity Purification and Mass Spectrometry Chin-Mei Lee, Christopher Adamchek, Ann Feke, Dmitri A. Nusinow, and Joshua M. Gendron 16. Measuring Protein Movement, Oligomerization State, and Protein-Protein Interaction in Arabidopsis Roots Using Scanning Fluorescence Correlation Spectroscopy (scanning FCS) Natalie M. Clark and Rosangela Sozzani 17. Studying Protein-Protein Interactions in planta using Advanced Fluorescence Microscopy Marc Somssich and Rudiger Simon 18. Chemiluminescence Based Detection of Peptide Activity and Peptide-Receptor Binding in Plants Mari Wildhagen, Markus Albert, and Melinka A. Butenko 19. Application of Chemical Genomics to Plant-Bacteria Communication: A High-Throughput System to Identify Novel Molecules Modulating the Induction of Bacterial Virulence Genes by Plant Signals Elodie Vandelle, Maria Rita Puttilli, Andrea Chini, Giulia Devescovi, Vittorio Venturi, and Annalisa Polverari

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詳細情報

• NII書誌ID(NCID): BB24301782
• ISBN:
  • 9781493970018
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xi, 317 p.
• 大きさ: 27 cm
• 親書誌ID:
  • BA10794673
  • BA88873632