Natural products from marine algae : methods and protocols
著者
書誌事項
Natural products from marine algae : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, 1308)(Springer protocols)
Humana Press, c2015
- : pbk
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注記
Includes bibliographical references and index
"Softcover reprint of the hardcover 1st edition 2015"--T.p. verso
内容説明・目次
内容説明
This volume provides a fundamental overview of the current state of the art in natural products from marine algae, linking the complex and diverse natural resource with recent developments in extraction, analytical and bioactivity testing methodologies. Natural Products from Marine Algae: Methods and Protocols guides readers through protocols and techniques on algal biotechnology, metabolites, Solid-Liquid Extraction (SLE), Microwave Assisted Extraction (MAE), Liquid Chromatography, Gas Chromatography, Nuclear Magnetic Resonance Spectroscopy, Infra-red spectroscopy and Raman Spectroscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Natural Products from Marine Algae: Methods and Protocols hopes to aid scientists unravel and quantify algal chemical diversity and support further marine biotechnological developments.
目次
1. Marine Algae a Source Of Biomass for Biotechnological Applications
Dagmar B Stengel and Solene Connan
2. Structure and Function of Macro algal Natural Products
Ryan M. Young, Kathryn M. Schoenrock, Jacqueline L. von Salm, Charles D. Amsler, and Bill J. Baker
3. Spectrophotometric Assays of Major Compounds Extracted From Algae
Solene Connan
4. Extraction and Enrichment of Protein From Red and Green Macroalgae
Padraigin A Harnedy and Richard. J. FitzGerald
5. Extraction and Purification of R-phycoerythrin from Marine Red Algae
Justine Dumay, Michele Morancais, Trang Nguyen Huu Phuo, and Joel Fleurence
6. Extraction and Analysis of Mycosporine-like Amino Acids in Marine Algae
Nedeljka N. Rosic, Christoph Braun, and David Kvaskoff
7. Extraction and Purification Of Phlorotannins from Brown Algae
Erwan Ar Gall, Florian Lelchat, Melanie Hupel, Camille Jegou, and Valerie Stiger-Pouvreau
8. Enzyme-enhanced Extraction Of Antioxidant Ingredients From Algae
Bjoern V. Adalbjoernsson and Rosa Jonsdottir
9. Microwave-assisted Extraction Of Fucoidan From Marine Algae
Solange I. Mussatto
10. Extraction and Analysis of Oxylipins from Macroalgae Illustrated on the Example Gracilaria vermiculophylla
Dominique Jacquemoud and Georg Pohnert
11. Lipids and Fatty Acids In Algae: Extraction, Fractionation Into Lipid Classes, and Analysis by Gas Chromatography Coupled with Flame Ionisation Detector (GC-FID)
Freddy Guiheneuf, Matthias Schmid, and Dagmar B. Stengel
12. HRMAS NMR Analysis Of Algae and Identification of Molecules of Interest Via Conventional 1D and 2D NMR: Sample Preparation and Optimization Of Experimental Conditions
Gaelle Simon, Nelly Kervarec, and Stephane
Cerantola
13. Extraction, Purification and NMR Analysis Of Terpenes From Brown Algae
Marc Gaysinski, Annick Ortalo-Magne, Olivier P. Thomas, and Gerald Culioli
14. Extraction, Isolation, and Identification of Sesquiterpenes From Laurencia Species
Angelica Ribeiro Soares
15. The use of HPLC for the Characterisation Of Phytoplankton Pigments
Jose L. Garrido and Suzanne Roy
16. Characterization of Phlorotannins from Brown Algae by LC-HRMS
Jeremy E. Melanson and Shawna L. MacKinnon
17. Analysis of Betaines From Marine Algae using LC-MS-MS
Shawna L. MacKinnon and Cheryl Craft
18. Analysis of Marine Biotoxins using LC-MS/MS
Bernd Luckas, Katrin Erler, and Bernd Krock
19. Fucoidan Analysis by Tandem MALDI-TOF and ESI Mass Spectrometry
Stanislav D. Anastyuk, Natalia M. Shevchenko, and Vladimir I. Gorbach
20. Determination of Substitution Patterns of Galactans from Green Seaweeds Of The Bryopsidales
Paula Ximena Arata, Paula Virginia Fernandez, and Marina Ciancia
21. Structural Characterization Of A Hybrid Carrageenan-Like Sulphated Galactan from a Marine Red Alga Furcellaria lumbricalis
Youjing Lv, Bo Yang, Xia Zhao, Junzeng Zhang, and Guangli Yu
22. Characterisation Of Alginates By Nuclear Magnetic Resonance (NMR) and Vibrational Spectroscopy (IR, NIR, Raman) in Combination with Chemometrics
Henrik Max Jensen, Flemming Hofmann Larsen, and Soren Balling Engelsen
23. Imaging and Identification of Marine Algal Bio-active Compounds by Surface Enhanced Raman Spectroscopy (SERS)Mats Josefson, Alexandra Walsh, and Katarina Abrahamsson
24. In vitro protocols for Measuring the Antioxidant Capacity of Algal Extracts Owen Kenny, Nigel P. Brunton, and Thomas J. Smyth
25. Disk
Diffusion Assay to Assess the Antimicrobial Activity of Marine Algal Extracts
Andrew P. Desbois and Valerie J. Smith
26. Screening of a Marine Algal Extract for Antifungal Activities
Graciliana Lopes, Paula B. Andrade, and Patricia Valentao
27. Protocol for Assessing Antifouling Activities of Macroalgal Extracts
Claire Hellio, Rozenn Trepos , R. Noemi Aguila-Ramirez, and Claudia J. Hernandez-Guerrero
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