Plant stress tolerance : methods and protocols
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Bibliographic Information
Plant stress tolerance : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1631）（Springer protocols）
Humana Press : Springer Nature, c2017
2nd ed
- : hard
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"This Humana Press imprint is published by Springer Nature. The registered company is Springer Science+Business Media LLC"--T.p. verso
Includes bibliographical references and index
Description and Table of Contents
Description
This second edition provides up-to-date and new chapters covering the most important widely-used techniques in plant stress tolerance, including cutting-edge strategies, in a manner that ensures effective results. Plant Stress Tolerance: Methods and Protocols, Second Edition guides readers through reviews on epigenetics, long noncoding RNAs, and the influence of microbiome on drought tolerance, the volume then continues with methods describing the identification of stress-responsive genes, proteins, posttranslational redox modifications, DNA methylation, histone modifications, measurement of glutathione, lipids, raffinose family oligosaccharides, polyamines, as well as quantification of abscisic acid, and the enzymes involved in ROS detoxification. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Stress Tolerance: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Table of Contents
Part I: Review Chapters
1. Epigenetics and RNA Processing: Connections to Drought, Salt and ABA?
Min May Wong, Geeng Loo Chong and Paul Verslues
2. The Fundamental Role Of Reactive Oxygen Species In Stress Response
Michael Liebthal and Karl-Josef Dietz
3. Role of Long Non-coding RNAs on Plant Stress Tolerance
Yuepeng Song and Deqiang Zhang
4. Towards a Resilient, Functional Microbiome: Drought Tolerance-Alleviating Microbes for Sustainable Agriculture
Venkatachalam Lakshmanan, Prasun Ray, and Kelly D. Craven
Part II: Methodology Chapters
5. Mining and Quantifying In Vivo Molecular Interactions in Abiotic Stress Acclimation
Thorsten Seidel and Derya Kirasi
6. Generation of a Stress-inducible Luminescent Arabidopsis and Its Use in Genetic Screening for Stress-responsive Gene Deregulation Mutants
Si-in Yu and Byeong-ha Lee
7. Detection of Differential DNA Methylation under Stress Conditions using Bisulfite-Sequence Analysis
Ibtisam Al Harrasi, Rashid Al-Yahyai and Mahmoud W Yaish
8. ChIP-seq Analysis for Identifying Genome-wide Histone Modifications Associated With Stress-responsive Genes in Plants
Guosheng Li, Guru Jagadeeswaran, Andrew Mort, and Ramanjulu Sunkar
9. Isolation of Polysomal RNA for Analyzing Stress-Responsive Genes Regulated at the Translational Level in Plants
Yong-Fang Li, Ramamurthy Mahalingam, and Ramanjulu Sunkar
10. Global Proteomic Profiling and Identification of Stress-Responsive Proteins using Two-dimensional Gel Electrophoresis
Pragya Barua, Deepak Gayen, Nilesh Vikram Lande, Subhra Chakraborty, and Niranjan Chakraborty
11. Phosphoproteomics Analysis for Probing Plant Stress Tolerance
Christof Rampitsch
12. Probing Posttranslational Redox Modifications
Patrick Treffon, Michael Liebthal, Wilena Telman, and Karl-Josef Dietz
13. Zymographic Method for Distinguishing Different Classes of Superoxide Dismutases in Plants
Ashwini R. Jamdhade, Ramanjulu Sunkar, and Vandana K. Hivrale
14. Determination of Enzymes Associated with Sulfite Toxicity in Plants: Kinetic Assays For SO, APR, SiR and in-gel SiR Activity
Galina Brychkova, Assylay Kurmanbayeva, Aizat Bekturova, Inna Khozin, Dominic Standing, Dmitry Yarmolinsky, and Moshe Sagi
15. Determination of Total Sulfur, Sulfate, Sulfite, Thiosulfate, and Sulfolipids in Plants
Assylay Kurmanbayeva, Galina Brychkova, Aizat Bekturova, Inna Khozin, Dominic Standing, Dmitry Yarmolinsky, and Moshe Sagi
16. Determining Glutathione Levels in Plans
Smita Sahoo, Jay Prakash Awasthi, Ramanjulu Sunkar, and Sanjib Kumar Panda
17. Porous Graphitic Carbon Liquid Chromatography-Mass Spectrometry Analysis of Drought Stress-responsive Raffinose Family Oligosaccharides in Plant Tissues
Tiago F. Jorge, Maria H. Florencio, and Carla Antonio
18. Profiling Abscisic Acid Induced Changes in Fatty Acid Composition in Mosses
Suhas Shinde, Shivakumar Devaiah and Aruna Kilaru
19. Detection of Free Polyamines in Plants Subjected to Abiotic Stresses by High-Performance Liquid Chromatography (HPLC)
Xiaoqing Gong and Jihong Liu
20. Determination of Polyamines by Dansylation, Benzoylation, and Capillary Electrophoresis
G. Veeranagamallaiah and Chinta Sudhakar
21. Rapid Quantification of Abscisic Acid by GC-MS/MS for studies of Abiotic Stress Response
Paul E. Verslues
22. Silencing of Stress-regulated miRNAs in Plants by Short Tandem Target Mimic (STTM) Approach
Sachin Teotia and Guiliang Tang
23. Rhizosphere Sampling Protocols for Microbiome (16S/18S/ITS rRNA) Library Preparation and Enrichment for the Isolation of Drought Tolerance-Promoting Microbes
Venkatachalam Lakshmanan, Prasun Ray, and Kelly D. Craven
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