Membrane protein structure and function characterization : methods and protocols
Author(s)
Bibliographic Information
Membrane protein structure and function characterization : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1635）（Springer protocols）
Humana Press : Springer Nature, c2017
- : hard
Available at / 1 libraries
-
No Libraries matched.
- Remove all filters.
Note
"This Humana Press imprint is published by Springer Nature. The registered company is Springer Science+Business media LLC"--T.p.verso
Includes bibliographical references and index
Description and Table of Contents
Description
In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowledge of guidelines and tricks may help to increase chances to express, purify and characterize a peculiar membrane protein. Production of correctly folded protein remains a challenge. Moreover, getting a functional and stable protein requires to optimize membrane mimicking environments that can be detergent or artificial membranes. In some cases, the finding of the correct ligand which will stabilize the desired conformation is needed. In other cases, stabilization can be obtained using specific antibodies. This volume also presents different techniques to analyze the functional status of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters in Membrane Protein Structure and Function Characterization: Methods and Protocols provide different techniques to analyze the functional and structural status of membrane proteins. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Membrane Protein Structure and Function Characterization: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Table of Contents
1. Recombinant Overexpression of Mammalian TSPO Isoforms 1 and 2 Lucile Senicourt, Soria Iatmanen-Harbi, Claude Hattab, Mariano A Ostuni, Marie-France Giraud, and Jean-Jacques Lacapere
2. Functional Assembly of Soluble and Membrane Recombinant Proteins of Mammalian NADPH Oxidase Complex
Hajer Souabni, Aymen Ezzine, Tania Bizouarn, and Laura Baciou
3. Direct Extraction and Purification of Recombinant Membrane Proteins from Pichia pastoris protoplasts
Lucie Hartmann, Estelle Metzger, Noemie Ottelard, and Renaud Wagner
4. Cell-free Expression for the Study of Hydrophobic Proteins: The Example of Yeast ATP-synthase subunits
Isabelle Larieu, James Tolchard, Corinne Sanchez, Edmond Yazo Kone, Alexandre Barras, Claire Stines-Chaumeil, Benoit Odaert, and Marie-France Giraud
5. Wheat Germ Cell Free Overexpression for the Production of Membrane Proteins
Marie-Laure Faugeron, Aurelie Badillo, Francois Penin, and Anja Bocjmann
6. Methyl-specific Isotope Labelling Strategies for NMR Studies of Membrane Proteins
Vilius Kurauskas, Paul Schanda, and Remy Sounier
7. Labeling of Membrane Complexes for Electron Microscopy
Francesca Gubellini and Remi Fronzes
8. Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins Case Study of The Mouse 5-HT Receptor
Gherici Hassaine, Cedric Deluz, Luigino Grasso, Romain Wyss, Ruud Hovius, Henning Stahlberg, Takashi Tomizaki, Aline Desmyter, Christophe Moreau, Lucie Peclinovska, Sonja Minniberger, Lamia Mebarki, Xiao-Dan Li, Horst Vogel, and Hugues Nury
9. Characterization of New Detergent and Detergent Mimetics by Scattering Techniques for Membrane Protein Crystallization
Francoise Bonnete and Patrick J Loll
10. Secondary Structure Determination by Means of ATR-FTIR Spectroscopy
Batoul Srour, Stefan Bruechert, Susana Andrade, and Petra Hellwig
11. Native Mass Spectroscopy for the Characterization of Structure and Interactions of Membrane Proteins
Jeroen F Van Dyck, Albert Konijnenberg, and Frank Sobott
12. Mass Spectroscopy of Mitochondrial Membrane Proteins Complexes
Luc NEGRONI, Michel ZIVY, and Claire LEMAIRE
13. Functional Studies on Membrane Proteins by Means of H/D Exchange in Infrared: Structural Changes in Na+NQR from V cholerae in the Presence of Lipids
Yashvin Neehaul, Sebastien Kriegel, Blanca Barquera, and Petra Hellwig
14. Reconstitution of Membrane Protein in Liposomes
Alice Verchere, Isabelle Broutin, and Martin Picard
15. Ion Channels as Reporters of Membrane Receptor Function: Automated Analysis in Xenopus Oocytes
Michel Vivaudou, Zlatomir Todorov, Gina Catalina Reyes-Mejia, and Christophe Moreau
16. The CRACAM Robot: Two Dimensional Crystallization of Membrane Protein
Philippe Rosier, Frederic Gelebart, Nicolas Dumesnil, Gauthier Esnot, Manuela Dezi, Marc Morand, and Catherine Venien-Bryan
17. Reconstitution of Membrane Proteins into Nanodiscs for Single Particle Electron Microscopy
Laetitia Daury, Jean-Christophe Taveau, Dimitri Salvador, Marie Glavier, and Olivier Lambert
18. Solid-state NMR of Membrane Protein Reconstituted in Proteoliposomes, the Case of TSPO
Lucile Senicourt, Luminita Duma, Vassilios Papadopoulos, and Jean-Jacques Lacapere
19. Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR
Denis Lacabanne, Britta Kunert, Carole Gardiennet, Beat H Meier, and Anja Bockmann
20. Simulation of Ligand Binding To Membrane Proteins
Samuel Murail
21. Molecular modelling of Transporters: From Low Resolution Cryo-Electron Microscopy Map to Conformational Exploration. The Example of TSPO Molecular Dynamic of Membrane Protein in Different Environments
Aurore Vaitinadapoule and Catherine Etchebest
by "Nielsen BookData"
