Environmental responses in plants : methods and protocols

著者

    • Duque, Paula

書誌事項

Environmental responses in plants : methods and protocols

edited by Paula Duque

（Methods in molecular biology / John M. Walker, series editor, 1398）（Springer protocols）

Humana Press, c2016

  • : pbk

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注記

"Humana Press is brand of Springer"--T.p. verso

Includes bibliographical references and index

"Softcover reprint of the hardcover 1st edition 2016" -- pbk. t.p. verso

内容説明・目次

内容説明

This volume describes different up-to-date methodological approaches, ranging from physiological assays to imaging and molecular techniques, to study a wide variety of plant responses to environmental cues. Environmental Responses in Plants: Methods and Protocols is divided into four sections: Tropisms, Photoperiodism and Circadian Rhythms, Abiotic Stress Responses, and Plant-Pathogen Interactions. The chapters in these sections include detailed protocols to investigate some of the many key biological processes underlying plant environmental responses, mostly in the model organism Arabidopsis thaliana, but also in Physcomitrella patens and in different crop species such as rice, potato, barley, or tomato. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Environmental Responses in Plants: Methods and Protocols, is a great resource for plant physiologists, biochemists, and cell and molecular scientists interested in this exciting and fast-growing research topic.

目次

PaPart I Tropisms 1.11. Hydrotropism: Analysis of the Root Response to a Moisture Gradient Regina Antoni, Daniela Dietrich, Malcolm J. Bennett, and Pedro L. Rodriguez 2. Assessing Gravitropic Responses in Arabidopsis Richard Barker, Benjamin Cox, Logan Silber, Arah Sangari, Amir Assadi, and Patrick Masson 3. Physiological Analysis of Phototropic Responses in Arabidopsis Mathias Zeidler 4. Automatic Chloroplast Movement Analysis Henrik Johansson and Mathias Zeidler 5. Micro scopic and Biochemical Visualization of Auxins in Plant Tissues Joshua J. Blakeslee and Angus S. Murphy 6. Immunolocalization of PIN and ABCB Transporters in Plants Nicola Carraro and Wendy A. Peer Part II Photoperiodism and Circasian Rhythms 7. Analysis of Circadian Leaf Movements Niels A. Muller and Jose M. Jimemez-Gomez 8. Sample Preparation of Arabidopsis Thaliana Shoot Apices for Expression Studies of Photoperiod-Induced Genes Fernando Andres, Stefano Torti, Coral Vincent, and George Coupland 9. A Luciferase-Based Assay to Test Whether Gene Expression Responses to Environmental Inputs are Temporally Restricted by the Circadian Clock Amaury de Montaigu, Markus C. Berns, and Geroge Coupland 10. Identification of Arabidopsis Transcriptional Regulators by Yeast One-Hyrbid Screens Using a Transcription Factor ORFeome Ghislain Breton, Steve A. Kay, and Jose L. Pruneda-Paz 11. Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes Craig G. Simpson, John Fuller, Cristiane P. G. Calixto, Jim McNicol, Clare Booth, John W. S. Brown, and Dorothee Staiger 12. Assessing the Impact of Photosynthetic Sugars in the Arabidopsis Circadian Clock Michael J. Haydon and Alex A. R. Webb 13. Assessing Protein Stability Under Different Light and Circadian Conditions Takatoshi Kiba and Rossana Henriques Part III Abiotic Stress Responses 14. Screening for Abiotic Stress Tolerance in Rice: Salt, Cold, and Drought Diego M. Almeida, M. Cecilia Almadanim, Tiago Louren co, Isabel A. Abreu, Nelson J. M. Saibo, and M. Margarida Oliveira 15. Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis Thaliana Urszula Piskurewicz and Luis Lopez-Molina 16. Assessing Tolerance to Heavy-Metal Stress in Arabidopsis Thaliana Seedings Estelle Remy and Paula Duque 17. Assessing Drought Responses Using Thermals Infrared Imaging Ankush Prashar and Hamlyn G. Jones 18. Generating Targeted Gene Knockout Lines in Physcomotrella Patens to Study Evolution of Stress- Responsive Mechanisms Monika Maronova and Maria Kalyna 19. Screening Stress Tolerance Traits in Arabidopsis Cell Cultures Imma P. Salamo, Bogata Boros, and L aszlo Szabados 20. Using Arabidopsis Protoplasts to Study Cellular responses to Environmental Stress Ana Confraria and Elena Baena-Gonzalez 21. Construction of Artificial miRNAs to Prevent Drought Stress in Solanum Tuberosum Anna Wyrzykowska, Marcin Pieczynski, and Zofia Szweykowska-Kulinska Part IV Plant-Pathogen Interactions 22. Virus-Induced Gene Silencing for Gene Function Studies in Barley Maria Barciszewska-Pacak, Artur Jarmolowski, and Andrzej Pacak 23. Methods for Long-Term Stable Storage of Colletotrichum Species Kei Hiruma and Yusuke Saijo 24. Plant Inoculation with the Fungal Leaf Pathogen Colletotrichum Higginsianum Kei Hiruma and Yusuke Saijo 25. Tracing Plant Defense Responses in Roots Upon MAMP/DAMP Treatment Kei Hiruma and Yusuke Saijo 26. Analysis of the Immunity=Related Oxidative Bursts by a Luminol-Based Assay Marco Trujillo 27. Quantitative Analysis of Microbe-Associated-Molecular-Pattern (MAMP)-Induced Ca2+ Transients in Plants Fabian Trempel, Stefanie Ranf, Dierk Scheel, and Justin Lee 28. Rapid Assessment of DNA Methylation Changes in Response to Salicylic Acid by Chop-qPCR Stephanie Rausch and Sascha Laubinger 29. Determining Nucleosome Position at Individual Loci after Biotic Stress Using MNase-qPCR Margaux Kaster and Sascha Laubinger 30. Phosphoprotein Enrichment Combined with Phosphopeptide Enrichment to Identify Putative Phosphoproteins During Defense Response in Arabidopsis Thaliana Ines Lassowskat, Wolfgang Hoehenwarter, Justin Lee, and Dierk Scheel 2. 2. 1 1 1 1.

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