Food allergens : methods and protocols

著者

    • Lin, Jing
    • Alcocer, Marcos J.C.

書誌事項

Food allergens : methods and protocols

edited by Jing Lin, Marcos Alcocer

（Methods in molecular biology / John M. Walker, series editor, 1592）（Springer protocols）

Humana Press, c2017

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This volume provides a collection of methodologies for basic research, clinical diagnosis, and treatment pertaining to food allergens, including food allergen production, purification, characterization, detection, quantification, and bioinformatics approaches to modern food allergen studies. The chapters in the book are divided into 4 parts: Part I discusses food allergen purification and production, and explores methods of producing recombinant food allergens in bacterial and yeast expression systems; Part II looks at allergen discovery, detection, and quantification covering 3 types of methods-DNA-, protein-, and cell-based methods; Part III focuses on allergenic epitope mapping; and Part IV talks about future developments concentrated around new concepts of allergenicity as an outcome of protein and food matrix interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Food Allergens: Methods and Protocols is a valuable resource for immunologists, biochemists, molecular biologists, and medical doctors and students working in the food allergy field. This book is also useful for people in the food industry, legislators, food standard agencies, allergologists, pediatricians, and clinicians in the allergic diseases and immunology fields.

目次

Preface... Table of Contents... Contributing Authors... 1: Overview of the Commonly used Methods for Food Allergens Jing Lin and Marcos J.C. Alcocer Part I Food Allergen Purification and Production 2: Allergen Extraction and Purification from Natural Products: Main Chromatographic Techniques Barbara Cases, Carlos Pastor-Vargas, and Marina Perez-Gordo 3: Recombinant Allergen Production in E. Coli Changqi Liu, LeAnna N. Willison, and Shridhar K. Sathe 4: Recombinant Allergens Production in Yeast Maria Neophytou and Marcos J.C. Alcocer Part II Food Allergen Discovery, Detection, and Quantification 5: 2D-Electrophoresis and Immunoblotting in Food Allergy Galina Grishina, Luda Bardina, and Alexander Grishin 6: Two Dimensional Electrophoresis and Identification by Mass Spectrometry (MS) Fernando de la Cyesta, Gloria Alvarez-Llamas, and Maria G. Barderas 7: Enzyme-Linked Immunosorbent Assay (ELISA) George N. Konstantinou 8: Detection of Food Allergens by Taqman Real-Time PCR Methodology Aina Garcia, Raquel Madrid, Teresa Garcia, Rosario Martin, and Isabel Gonzalez 9: Detection of Food Allergens by Phage Displayed Produced Antibodies Raquel Madrid, Silvia de la Cruz, Aina Garcia, Rosario Martin, Isabel Gonzalez, and Teresa Garcia 10: Protein Microarray-Based IgE-Immunoassay for Allergy Diagnosis Nuzul N. Jambari, XiaoWei Wang, and Marcos J.C. Alcocer 11: Basophil Degranulation Assay Madhan Masilamani, Mohanapriya Kamalakannan, and Hugh A. Sampson 12: Use of Humanized RS-ATL8 Reporter System for Detection of Allergen-Specific IgE Sensitization in Human Food Allergy Eman Ali Ali, Ryosuke Nakamura, and Franco H. Falcone Part III Allergenic Epitope Mapping 13: Assessment of IgE Reactivity of -Casein by Western Blotting after Digestion with Simulated Gastric Fluid Sara Benede, Rosina Lopez-Fandino, and Elena Molina 14: IgE Epitope Mapping using Peptide Microarray Immunoassay Jing Lin and Hugh A. Sampson 15: T Cell Proliferation Assay: Determination of Immunodominant T Cell Epitopes of Food Allergens Madhan Masilamani, Mariona Pascal, and Hugh A. Sampson 16: Tetramer Guided Epitope Mapping: A Rapid Approach to Identify HLA-Restricted T-Cell Epitopes from Composite Allergens Luis Diego Archila and William W. Kwok 17: T-Cell Epitope Prediction George N. Konstantinou 18: An Overview of Bioinformatics Tools and Resources in Allergy Zhiyan Fu and Jing Lin Part IV Methods Currently being Developed and Future Development 19: The Use of a Semi-Automated System to Measure Mouse Natural Killer T (NKT) Cell Activation by Lipid Loaded Dendritic Cells Ashfaq Ghumra and Marcos J.C. Alcocer 20: Recent Advances in the Detection of Allergens in Foods Silvia de la Crus, Ines Lopez-Calleja, Rosario Martin, Isabel Gonzalez, Marcos J.C. Alcocer, and Teresa Garcia

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