G protein-coupled receptors

著者

書誌事項

G protein-coupled receptors

edited by Arun K. Shukla

（Methods in cell biology / edited by David M. Prescott, v. 142, 149）

Academic Press, an imprint of Elsevier, 2017-

2nd ed

  • pt. A
  • pt. B

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注記

Includes bibliographical references and index

内容説明・目次

巻冊次

pt. A ISBN 9780128133194

内容説明

G Protein Coupled Receptors, Second Edition, Volume 143, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. It contains a wide array of topics about the G protein coupled receptors, as well as updates of chapters from the first edition.

目次

1. Generating recombinant peptide ligands for GPCRs and their single step purification 2. Ca2+ release assay to study downstream pathways of GPCR signal transduction 3. Expression and purification of GPCR interacting protein, arrestin 4. Isolation and characterization of chemosensory GPCRs from exosomes 5. Characterization of chemosensory bitter taste receptor signalling 6. Reporter gene assays for investigating GPCR signaling 7. Measuring binding affinity of regulatory motifs (peptides) to purified G proteins 8. Measuring nucleotide exchange on purified G proteins 9. Qualitative and quantitative measurement of GPCR-G protein activity in living cells 10. Computational approaches to study GPCR activation 11. Visualization and quantification of GPCR trafficking in mammalian cells by confocal microscopy 12. Computational studies of ssarrestin activation 13. Measuring G protein coupling using Glosensor assay 14. Computational investigations into GPCR dimerization 15. cAMP Assays in GPCR Drug Discovery 16. Assays of adrenal GPCR signaling and regulation: Measuring adrenal beta-arrestin activity in vivo through plasma membrane recruitment
巻冊次

pt. B ISBN 9780128151075

内容説明

G-Protein-Coupled Receptors, Part B, 2nd Edition, Volume 149, the latest release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers Optical Approaches for Visualization of Arrestin Binding to Muscarinic Receptors, Luciferase Reporter Assay for Unlocking Ligand-mediated Signaling of GPCRs, Assays to Measure GPCR Dependent Cellular Migration, Characterization of the Frizzled GPCRs, Binding Assays for Bradykinin and Angiotensin Receptors, Detection of Misfolded Rhodopsin Aggregates in Cells, Measuring GPCR Ubiquitination and Trafficking, Culture of Primary Neurons and its Use in Studying GPCR Trafficking, and much more.

目次

1. Optical approaches for visualization of arrestin binding to muscarinic receptor Seung Ryoung Jung 2. Luciferase Reporter Assay for Unlocking Ligand-mediated Signaling of GPCRs Hamiyet Unal 3. Assays to measure GPCR dependent cellular migration Carole A. Parent 4. Characterization of the Frizzled GPCRs Vladimir L. Katanaev 5. Binding assays for bradykinin and angiotensin receptors Renan Martin 6. Detection of misfolded rhodopsin aggregates in cells Paul Park 7. Measuring GPCR ubiquitination and trafficking Samarjit Bhattacharyya 8. Culture of primary neurons and its use in studying GPCR trafficking Prem N. Yadav 9. ELISA based assay for measuring agonist-induced GPCR endocytosis Arun K. Shukla 10. Western blot assay for measuring agonist-induced ERK1/2 phosphorylation Arun K. Shukla 11. Understanding GPCR dimerization Agata Faron-Gorecka 12. Investigating the Frizzled family of receptors Vladimir L. Katanaev 13. Measuring GPCR signaling through xCELLigence system based assay Laura Heitman 14. Novel mechanisms of GPCR signaling and bias Gabriel Carmona Rosas 15. co-IP assays to measure GPCR-arrestin interactions Anastasios Lymperopoulos 16. Investigating arrestin dependent GPCR signaling Sadashiva Karnik 17. Measuring CRH1 receptor signaling Susana Silberstein 18. Site-specific side chain labeling for NMR studies of G protein-coupled receptors Brian F. Volkman 19. Modeling chemokine receptors and their orthosteric site interactions Brian F. Volkman 20. Receptor-channel cross-talk Uri Kahanovitch 21. Wnt receptor signaling Nibaldo C. Inestrosa

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  • Methods in cell biology

    edited by David M. Prescott

    Academic Press 1964-

    v. 1 , v. 2 , v. 3 , v. 4 , v. 5 , v. 6 , v. 7 , v. 8 , v. 9 , v. 10 , v. 11 , v. 12 , v. 13 , v. 14 , v. 15 , v. 20 , v. 21A , v. 21B , v. 24 , v. 25 , v. 26 , v. 27 , v. 28 , v. 29 , v. 30 , v. 31 , v. 32 , v. 33 , v. 34 , v. 35 , v. 36 , v. 40 , v. 44 , v. 54 , v. 72 , v. 73

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