Long non-coding RNAs : methods and protocols

著者

    • Feng, Yi
    • Zhang, Lin

書誌事項

Long non-coding RNAs : methods and protocols

edited by Yi Feng, Lin Zhang

（Methods in molecular biology / John M. Walker, series editor, 1402）（Springer protocols）

Humana Press, c2016

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This volume assembles a broad spectrum of methods used in long non-coding RNAs (lncRNA) research, ranging from computational annotation of lncRNA genes to molecular and cellular analyses of the function of individual lncRNA. Long Non-Coding RNAs: Methods and Protocols also discusses methods used to study circular RNAs and RNA splicing, as well as influential findings on lncRNA in human diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Long Non-Coding RNAs: Methods and Protocols is a must-have for molecular biologists, cell and developmental biologists, specialists who conduct disease-oriented research, and bioinformatics experts who seek a better understanding on lncRNA expression and function by computational analysis of the massive sequencing data that are rapidly accumulating in recent years.

目次

1. LncRNA Pulldown Combined with Mass Spectrometry to Identify the Novel LncRNA-Associated Proteins Zhen Xing, Chunru Lin, and Liuqing Yang 2. Cross-Linking Immunoprecipitation and qPCR (CLIP-qPCR) Analysis to Map Interactions between Long Non-Coding RNAs and RNA-Binding Proteins Je-Hyun Yoon and Myriam Gorospe 3. Characterization of Long Non-Coding RNA Associated Proteins by RNA-Immunoprecipitation Youyou Zhang, Yi Feng, Zhongyi Hu, Xiaowen Hu, Chao-Xing Yuan, Yi Fan, Lin Zhang 4. Isolation of Protein Complexes Associated with Long Non-Coding RNAs Kiranmai Gumireddy, Jinchun Yan, and Qihong Huang 5. Profiling Long Non-Coding RNA Expression Using Custom-Designed Microarray Xinna Zhang, Gabriel Lopez-Berestein, Anil K. Sood, and George A. Calin 6. Long Non-Coding RNA Expression Profiling Using Arraystar LncRNA Microarrays Yanggu Shi and Jindong Shang 7. Nuclear RNA Isolation and Sequencing Navroop K. Dhaliwal and Jennifer A. Mitchell 8. Targeted LncRNA Sequencing with the SeqCap RNA Enrichment System John C. Tan, Venera D. Bouriakov, Liang Feng, Todd A. Richmond, and Daniel Burgess 9. ChIP-Seq: Library Preparation and Sequencing Karyn L. Sheaffer and Jonathan Schug 10. Stellaris (R) RNA Fluorescence In Situ Hybridization for the Simultaneous detection of Immature and Mature Long Non-Coding RNAs in Adherent Cells Arturo V. Orjalo, Jr., and Hans E. Johansson 11. Simultaneous RNA-DNA FISH Lan-Tian Lai, Zhenyu Meng, Fangwei Shao & Li-Feng Zhang 12. Visualizing Long Non-Coding RNAs on Chromatin Michael Hinten, Emily Maclary, Srimonta Gayen, Clair Harris, and Sundeep Kalantry 13. Non-Isotopic Method for In Situ LncRNA Visualization and Quantitation Botoul Maqsodi and Corina Nikoloff 14. Detection of Long Non-Coding RNA Expression by Non-Radioactive Northern Blots Xiaowen Hu, Yi Feng, Zhongyi Hu, Youyou Zhang, Chao-Xing Yuan, Xiaowei Xu, and Lin Zhang 15. Assessment of In Vitro SiRNA Delivery in Cancer Mouse Models Hiroto Hatakeyama, Sherry Y. Wu, Lingegowda S. Mangala, Gabriel Lopez-Berestein, and Anil K. Sood 16. Targeting Long Non-Coding RNA with Antisense Oligonucleotide Technology as Cancer Therapeutics Tianyuan Zhou, Youngsoo Kim, and A. Robert Macleod 17. Characterization of Circular RNAs Yang Zhang, Li Yang, and Ling-Ling Chen 18. Methods for Characterization of Alternative RNA Splicing Samuel E. Harvey and Chonghui Cheng 19. NONCODEv4: Annotation of Non-Coding RNAs with Emphasis on Long Non-Coding RNAs Yi Zhao, Jiao Yuan, and Runsheng Chen 20. Computational Analysis of LncRNA from cDNA Sequences Susan Boerner and Karen M. McGinnis 21. Analyzing MiRNA: LncRNA Interactions Maria D. Paraskevopoulou and Artemis G. Hatzigeoriou 22. Long Non-Coding RNAs: An Overview Dongmei Zhang, Minmin Xiong, Congjian Xu, Peng Xiang, and Xiaomin Zhong

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