Bacterial protein secretion systems : methods and protocols

著者

• Journet, Laure
• Cascales, Eric

書誌事項

Bacterial protein secretion systems : methods and protocols

editors, Laure Journet and Eric Cascales

（Methods in molecular biology / John M. Walker, series editor, 1615）（Springer protocols）

Humana Press, c2017

注記

Includes bibliographical references and index

内容説明・目次

内容説明

This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but are also of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope.

目次

1. Identification of Protein Secretion Systems in Bacterial Genomes using MacSyFinder Sophie S Abby and Eduardo PC Rocha 2. Protein Sorting Prediction Henrik Nielsen 3. Cell Fractionation Melissa Petiti, Laetitia Houot, and Denis Duche 4. Defining Lipoprotein Localisation by Fluorescence Microscopy Maria Guillermina Casabona, Mylene Robert-Genthon, Didier Grunwald, and Ina Attree 5. Components Sub-cellular Localization: Identification of Lipoproteins using Globomycin and Radioactive Palmitate Nienke Buddelmeijer 6. Defining Membrane Protein Localization by Isopycnic Density Gradients Rhys A. Dunstan, Iain D. Hay, and Trevor Lithgow 7. Components Sub-cellular Localisation: Cell Surface Exposure Anna Konovalova 8. Probing Inner Membrane Protein Topology by Proteolysis Maxence S. Vincent and Eric Cascales 9. Mapping of Membrane Protein Topology by Substituted Cysteine Accessibility Method (SCAMTM) Mikhail Bogdanov 10. Defining Membrane Protein Topology using pho-lac Reporter Fusions Gouzel Karimova and Daniel Ladant 11. In vivo and in vitro Protein-peptidoglycan Interactions Gang Li and S. Peter Howard 12. Measure of Peptidoglycan Hydrolase Activity Yoann G. Santin and Eric Cascales 13. Protein-protein Interaction: Bacterial Two-Hybrid Gouzel Karimova, Emilie Gauliard, Marilyne Davi, Scot P. Ouellette, and Daniel Ladant 14. Protein-protein Interactions: Yeast two-hybrid Jer-Sheng Lin and Erh-Min Lai 15. Protein-protein Interactions: Cytology Two-hybrid Krishnamohan Atmakuri 16. Fusion Reporter Approaches to Monitor Transmembrane Helix Interactions in Bacterial Membranes Laureen Logger, Abdelrahim Zoued, and Eric Cascales 17. Protein-protein Interactions: Co-immunoprecipitation Jer-Sheng Lin and Erh-Min Lai 18. Protein-protein Interaction: Tandem Affinity Purification in Bacteria Julie P.M. Viala and Emmanuelle Bouveret 19. Site-directed and Time-resolved Photocrosslinking in Cells Metabolically Labeled With Radioisotopes Raffaele Ieva 20. Protein-protein Interactions: Pull-down Assays Arthur Louche, Suzana P. Salcedo, and Sarah Bigot 21. Protein-protein interactions: Surface Plasmon Resonance Badreddine Douzi 22. Assessing Energy-dependent Protein Conformational Changes in the TonB System Ray A. Larsen 23. Defining Assembly Pathways by Fluorescence Microscopy Abdelrahim Zoued and Andreas Diepold 24. Large Complexes: Cloning Strategy, Production, and Purification Eric Durand and Roland Lloubes 25. Shearing and Enrichment of Extracellular Type IV Pili Alba Katiria, Gonzalez Rivera, and Katrina T. Forest 26. Blue Native PAGE Analysis of Bacterial Secretion Complexes Susann Zilkenat, Tobias Dietsche, Julia V. Monjaras Feria, Claudia E. Torres-Vargas, Mehari Tesfazgi Mebrhatu, and Samuel Wagner 27. In situ Imaging of Bacterial Secretion Systems by Electron Cryo-tomography Gregor L. Weiss, Joao M. Medeiros, and Martin Pilhofer 28. Structural Analysis of Protein Complexes by Cryo Electron Microscopy Tiago R. D. Costa, Athanasios Ignatiou, and Elena V. Orlova 29. Bacterial Filamentous Appendages Investigated by Solid-state NMR Spectroscopy Birgit Habenstein and Antoine Loquet 30. Identification of Effectors: Precipitation of Supernatant Material Nicolas Flaugnatti and Laure Journet 31. Energy Requirements for Protein Secretion via the Flagellar type-III Secretion System Marc Erhardt 32. Screening for Secretion of the Type VI Secretion Protein Hcp by Enzyme-Linked Immunosorbent Assay and Colony Blot Brent S. Weber, Pek Man Ly, and Mario F. Feldman 33. Effector Translocation: Cya Reporter Assay Suma Chakravarthy, Bethany Huot, and Brian H. Kvitko 34. Monitoring Effector Translocation with the TEM-1 Beta-lactamase Reporter System Julie Allombert, Anne Vianney, and Xavier Charpentier 35. Effector Translocation Assay: Differential Solubilization Irina S. Franco, Sara V. Pais, Nuno Charro, and Luis Jaime Mota 36. Quantitative Determination of Anti-bacterial Activity during Bacterial Co-culture Juliana Alcoforado Diniz, Birte Hollmann, and Sarah Coulthurst

「Nielsen BookData」 より

詳細情報

• NII書誌ID(NCID): BB25062836
• ISBN:
  • 9781493970315
• LCCN: 2017944103
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xv, 528 p.
• 大きさ: 27 cm
• 分類:
  • DC23 : 572.64
• 件名:
  • LCSH : Protein -- Protein interactions -- Research -- Methodology
  • LCSH : Secretion
  • LCSH : Microbiology
• 親書誌ID:
  • BA10794673
  • BA88873632