Human T-lymphotropic viruses : methods and protocols

著者

    • Casoli, Claudio

書誌事項

Human T-lymphotropic viruses : methods and protocols

edited by Claudio Casoli

(Methods in molecular biology / John M. Walker, series editor, 1582)(Springer protocols)

Humana Press, c2017

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

While HIV-1 continues to be well-researched, this detailed volume draws attention to other members of the Retrovirus family, namely the Human T-lymphotropic Viruses (HTLVs), featuring the most updated technical information about HTLV determination and the methods to investigate their interaction with the host immune system and interfering pathogens. The contents include essential aspects of epidemiology and virus transmission, novel and robust methodologies for studying the effects of trans-activating regulatory HTLVs' proteins, the latest techniques for genotyping and gene expression analysis, as well as cellular phenotype and dynamics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Human T-Lymphotropic Viruses: Methods and Protocols serves as an ideal guide to an area of study that is very much worthy of further research.

目次

Part I: Epidemiology and Transmission 1. Serological and Molecular Methods to Study Epidemiological Aspects of Human T-Cell Lymphotropic Virus Type 1 Infection Olivier Cassar and Antoine Gessain 2. Molecular Epidemiology Database for Sequence Management and Data Mining Thessika Hiala Almeida Araujo, Filipe Ferreira de Almeida Rego, and Luiz Carlos Junior Alcantara 3. Reporter Systems to Study HTLV-1 Transmission Christine Gross and Andrea K. Thoma-Kress 4. Quantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Infection Using Co-Culture with Jurkat LTR-Luciferase or Jurkat LTR-GFP Reporter Cells Sandrine Alais, Helene Dutartre, and Renaud Mahieux 5. Isolation of Exosomes from HTLV-Infected Cells Robert A. Barclay, Michelle L. Pleet, Yao Akpamagbo, Kinza Noor, Allison Mathiesen, and Fatah Kashanchi Part II: Promoter Activity of HTLVs' Proteins 6. A Luciferase Functional Quantitative Assay for Measuring NF-qB Promoter Transactivation Mediated by HTLV-1 and HTLV-2 Tax Proteins Elisa Bergamo, Erika Diani, Umberto Bertazzoni, and Maria Grazia Romanelli 7. Generation of a Tet-On Expression System to Study Transactivation Ability of Tax-2 Fabio Bignami, Riccardo Alessio Sozzi, Umberto Bertazzoni, and Elisabetta Pilotti 8. EGF Uptake and Degradation Assay to Determine the Effect of HTLV Regulatory Proteins on the ESCRT-Dependent MVB Pathway Colin Murphy and Noreen Sheehy Part III: Genotyping and Gene Expression 9. Methods for Identifying and Examining HTLV-1 HBZ Post-Translational Modifications Jacob Al-Saleem, Mamuka Kvaratskhelia, and Patrick L. Green 10. High-Throughput Mapping and Clonal Quantification of Retroviral Integration Sites Nicolas A. Gillet, Anat Melamed, and Charles R.M. Bangham 11. STR Profiling of HTLV-1-Infected Cell Lines Vittoria Raimondi, Sonia Minuzzo, Vincenzo Ciminale, and Donna M. D'Agostino 12. Expression of HTLV-1 Genes in T-cells Using RNA Electroporation Mariangela Manicone, Francesca Rende, Ilaria Cavallari, Andrea K. Thoma-Kress, and Vincenzo Ciminale Part IV: Cellular Dynamics 13. Quantification of Cell Turnover in the Bovine Leukemia Virus Model Alix de Brogniez, Pierre-Yves Barez, Alexandre Carpentier, Geronimo Gutierrez, Michal Reichert, Karina Trono, and Luc Willems 14. Analysis of NK Cell Function and Receptor Expression During HTLV-1 and HTLV-2 Infection Federica Bozzano, Francesco Marras, and Andrea De Maria Part V: Therapy 15. Overview of Targeted Therapies for Adult T-Cell Leukemia/Lymphoma Rihab Nasr, Ambroise Marcais, Olivier Hermine, and Ali Bazarbachi

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