Human T-lymphotropic viruses : methods and protocols
著者
書誌事項
Human T-lymphotropic viruses : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1582）（Springer protocols）
Humana Press, c2017
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
While HIV-1 continues to be well-researched, this detailed volume draws attention to other members of the Retrovirus family, namely the Human T-lymphotropic Viruses (HTLVs), featuring the most updated technical information about HTLV determination and the methods to investigate their interaction with the host immune system and interfering pathogens. The contents include essential aspects of epidemiology and virus transmission, novel and robust methodologies for studying the effects of trans-activating regulatory HTLVs' proteins, the latest techniques for genotyping and gene expression analysis, as well as cellular phenotype and dynamics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Human T-Lymphotropic Viruses: Methods and Protocols serves as an ideal guide to an area of study that is very much worthy of further research.
目次
Part I: Epidemiology and Transmission
1. Serological and Molecular Methods to Study Epidemiological Aspects of Human T-Cell Lymphotropic Virus Type 1 Infection
Olivier Cassar and Antoine Gessain
2. Molecular Epidemiology Database for Sequence Management and Data Mining
Thessika Hiala Almeida Araujo, Filipe Ferreira de Almeida Rego, and Luiz Carlos Junior Alcantara
3. Reporter Systems to Study HTLV-1 Transmission
Christine Gross and Andrea K. Thoma-Kress
4. Quantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Infection Using Co-Culture with Jurkat LTR-Luciferase or Jurkat LTR-GFP Reporter Cells
Sandrine Alais, Helene Dutartre, and Renaud Mahieux
5. Isolation of Exosomes from HTLV-Infected Cells
Robert A. Barclay, Michelle L. Pleet, Yao Akpamagbo, Kinza Noor, Allison Mathiesen, and Fatah Kashanchi
Part II: Promoter Activity of HTLVs' Proteins
6. A Luciferase Functional Quantitative Assay for Measuring NF-qB Promoter Transactivation Mediated by HTLV-1 and HTLV-2 Tax Proteins
Elisa Bergamo, Erika Diani, Umberto Bertazzoni, and Maria Grazia Romanelli
7. Generation of a Tet-On Expression System to Study Transactivation Ability of Tax-2
Fabio Bignami, Riccardo Alessio Sozzi, Umberto Bertazzoni, and Elisabetta Pilotti
8. EGF Uptake and Degradation Assay to Determine the Effect of HTLV Regulatory Proteins on the ESCRT-Dependent MVB Pathway
Colin Murphy and Noreen Sheehy
Part III: Genotyping and Gene Expression
9. Methods for Identifying and Examining HTLV-1 HBZ Post-Translational Modifications
Jacob Al-Saleem, Mamuka Kvaratskhelia, and Patrick L. Green
10. High-Throughput Mapping and Clonal Quantification of Retroviral Integration Sites
Nicolas A. Gillet, Anat Melamed, and Charles R.M. Bangham
11. STR Profiling of HTLV-1-Infected Cell Lines
Vittoria Raimondi, Sonia Minuzzo, Vincenzo Ciminale, and Donna M. D'Agostino
12. Expression of HTLV-1 Genes in T-cells Using RNA Electroporation
Mariangela Manicone, Francesca Rende, Ilaria Cavallari, Andrea K. Thoma-Kress, and Vincenzo Ciminale
Part IV: Cellular Dynamics
13. Quantification of Cell Turnover in the Bovine Leukemia Virus Model
Alix de Brogniez, Pierre-Yves Barez, Alexandre Carpentier, Geronimo Gutierrez, Michal Reichert, Karina Trono, and Luc Willems
14. Analysis of NK Cell Function and Receptor Expression During HTLV-1 and HTLV-2 Infection
Federica Bozzano, Francesco Marras, and Andrea De Maria
Part V: Therapy
15. Overview of Targeted Therapies for Adult T-Cell Leukemia/Lymphoma
Rihab Nasr, Ambroise Marcais, Olivier Hermine, and Ali Bazarbachi
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