Functional genomics : methods and protocols
Author(s)
Bibliographic Information
Functional genomics : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1654）（Springer protocols）
Humana Press, c2017
3rd ed
Available at / 3 libraries
-
No Libraries matched.
- Remove all filters.
Note
"This Human Press imprint is published by Springer nature. The registered company is Springer Science+Business Media, LLS"--T.p. verso
Includes bibliographical references and index
Description and Table of Contents
Description
Reflecting developments in genome editing, this third edition volume fully updates a collection of key techniques for the study of functional genomics. The book is broken up into sections on bioinformatics, DNA, RNA, and protein analysis, as well as a closing section entitled "From Genotype to Phenotype." Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Updated and authoritative, Functional Genomics: Methods and Protocols, Third Edition seeks to aid scientists in establishing or extending technologies and techniques in their laboratories.
Table of Contents
Part I: Bioinformatics
1. Predicting RNA Structure with Vfold
Chenhan Zhao, Xiaojun Xu, and Shi-Jie Chen
2. RNA Function Prediction
Yongsheng Li, Juan Xu, Tingting Shao, Yunpeng Zhang, Hong Chen, and Xia Li
3. Computational Prediction of Novel miRNAs from Genome-Wide Data
Georgina Stegmayer, Cristian Yones, Laura Kamenetzky, Natalia Macchiaroli, and
Diego H. Milone
4. Protein Structure Modeling with MODELLER
Benjamin Webb and Andrej Sali
5. Protein Function Prediction
Leonardo Magalhaes Cruz, Sheyla Trefflich, Vinicius Almir Weiss, and Mauro Antonio Alves Castro
Part II: DNA Analysis
6. Capturing Three-Dimensional Genome Organization in Individual Cells by Single-Cell Hi-C
Takashi Nagano, Steven W. Wingett, and Peter Fraser
7. Genome-Wide Cell Type-Specific Mapping of In Vivo Chromatin Protein Binding Using an FLP-Inducible DamID System in Drosophila
Alexey V. Pindyurin
8. DNA Methylation Profiling Using Long-Read Single-Molecule Real-Time Bisulfite Sequencing (SMRT-BS)
Yao Yang and Stuart A. Scott
9. Copy Number Variation Analysis by Droplet Digital PCR
Suvi K. Harmala, Robert Butcher, and Chrissy H. Roberts
10. MicroScale Thermophoresis: A Rapid and Precise Method to Quantify Protein-Nucleic Acid Interactions in Solution
Adrian Michael Muller, Dennis Breitsprecher, Stefan Duhr, Philipp Baaske, Thomas Schubert, and Gernot Langst
11. Establishment of the CRISPR/Cas9 System for Targeted Gene Disruption and Gene Tagging
Eric Ehrke-Schulz, Maren Schiwon, Claudia Hagedorn, and Anja Ehrhardt
Part III: RNA Analysis
12. Holistic and Affordable Analyses of MicroRNA Expression Profiles Using Tagged cDNA Libraries and a Multiplex Sequencing Strategy
Patrick P. Weil, Yan Jaszczyszyn, Anne Baroin-Tourancheau, Jan Postberg, and Laurence Amar
13. MicroRNA Expression Analysis Using Small RNA Sequencing Discovery and RT-qPCR-Based Validation
Alan Van Goethem, Pieter Mestdagh, Tom Van Maerken, and Jo Vandesompele
14. Using Firefly (R) Particle Technology for Multiplex MicroRNA Profiling Without RNA Purification
Michael R. Tackett and Izzudin Diwan
15. Multiplex Real-Time PCR Using Encoded Microparticles for MicroRNA Profiling
Seungwon Jung and Sang Kyung Kim
16. Optimized Whole Transcriptome Profiling of Motor Axons
Lena Saal, Michael Briese, and Michael Sendtner
Part IV: Protein Analysis
17. 2D-DIGE in Proteomics
Matias Pasquali, Tommaso Serchi, Sebastien Planchon, and Jenny Renaut
18. STAGE-Diging in Proteomics
Paolo Soffientini and Angela Bachi
19. Protein Arrays I: Antibody Arrays
Yulin Yuan, Zuan-Tao Lin, Hongting Wang, Xia Hong, Mikala Heon, and Tianfu Wu
20. Protein Arrays II: Antigen Arrays
Yulin Yuan, Hongting Wang, Zuan-Tao Lin, Xia Hong, Mikala Heon, and Tianfu Wu
21. Protein Arrays III: Reverse-Phase Protein Arrays
Yulin Yuan, Xia Hong, Zuan-Tao Lin, Hongting Wang, Mikala Heon, and Tianfu Wu
22. Isolation of Exosomes for the Purpose of Protein Cargo Analysis with the Use of Mass Spectrometry
Monika Pietrowska, Sonja Funk, Marta Gawin, Lukasz Marczak, Agata Abramowicz, Piotr Widlak, and Theresa Whiteside
Part V: From Genotype to Phenotype
23. Virus-Induced Gene Silencing (VIGS) and Foreign Gene Expression in Pisum sativum L. Using the 'One-Step' Bean pod mottle virus (BPMV) Viral Vector
Chouaib Meziadi, Sophie Blanchet, Valerie Geffroy, and Stephanie Pflieger
24. Re-Expressing Epigenetically Silenced Genes by Inducing DNA Demethylation through Targeting of Ten-Eleven Translocation 2 to Any Given Genomic Locus
Julio Cesar Rendon, David Cano-Rodriguez, and Marianne G. Rots
25. Knockdown of Rice MicroRNA166 by Short Tandem Target Mimic (STTM)
Sachin Teotia, Dabing Zhang, and Guiliang Tang
26. RNAi-Mediated Knockdown of Protein Expression
Volker Baumann, Cornelia Lorenzer, Michael Thell, Anna-Maria Winkler, and Johannes Winkler
27. Engineered Zinc Finger DNA-Binding Domains: Synthesis, Assessment of DNA-Binding Affinity, and Direct Protein Delivery to Mammalian Cells
Mir A. Hossain, Isaac J. Knudson, Shaleen Thakur, Yong Shen, Jared R. Stees, Joeva J. Barrow, and Joerg Bungert
28. Production, Purification, and Titration of First-Generation Adenovirus Vectors
Ramona F. Kratzer and Florian Kreppel
by "Nielsen BookData"
