Innate antiviral immunity : methods and protocols
著者
書誌事項
Innate antiviral immunity : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, 1656)(Springer protocols)
Humana Press, c2017
大学図書館所蔵 全2件
  青森
  岩手
  宮城
  秋田
  山形
  福島
  茨城
  栃木
  群馬
  埼玉
  千葉
  東京
  神奈川
  新潟
  富山
  石川
  福井
  山梨
  長野
  岐阜
  静岡
  愛知
  三重
  滋賀
  京都
  大阪
  兵庫
  奈良
  和歌山
  鳥取
  島根
  岡山
  広島
  山口
  徳島
  香川
  愛媛
  高知
  福岡
  佐賀
  長崎
  熊本
  大分
  宮崎
  鹿児島
  沖縄
  韓国
  中国
  タイ
  イギリス
  ドイツ
  スイス
  フランス
  ベルギー
  オランダ
  スウェーデン
  ノルウェー
  アメリカ
注記
"This Human Press imprint is published by Springer Nature. The registered company is Springer Science+Business Media LLS"--T.p. verso
Includes bibliographical references and index
内容説明・目次
内容説明
This book explores methods to study the complex and evolving interplay between a virus and its host that range from model systems to the detection of chemical molecules. The collection starts with the application of humanized mice and zebrafish as model organisms to study virus-host interactions and induction of innate immune responses. Subsequent chapters outline diverse methods to detect small interfering RNAs, microRNAs, and virus-derived dsRNA from a variety of cells, tissues, and organisms, as well as to interrogating the cytosolic RNA and DNA sensing pathways, including using RNA PAMPs as molecular tools, purification of cGAMP from virus particles and infected cells, and mechanisms to visualize the subcellular localization and activation of the adaptor proteins MAVS and STING. Cutting-edge methods, including high-throughput and genome-wide CRISPR/Cas9 screens, chromosome conformation capture, and whole-exome sequencing, are described to identify novel mediators, pathways, and variants underlying host susceptibility. Given the importance of studying these pathways and players under physiologic conditions, methods describing the isolation of primary mouse sensory neurons and group 2 innate lymphoid cells are also provided. Finally, this collection comes full circle back to the whole organism level and concludes with epidemiological methods to investigate virus-host interactions and the induction of innate immunity. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Innate Antiviral Immunity: Methods and Protocols spans a diverse array of approaches to study and elucidate the intricacies of this vital area of study.
The chapter 'Morphological Separation of Clustered Nuclei in Histological Images' is published open access under a CC BY 4.0 license at link.springer.com.
目次
1. The Application of Humanized Mouse Models for the Study of Human Exclusive Viruses
Fatemeh Vahedi, Elizabeth C. Giles, and Ali A. Ashkar
2. Zebrafish as a Model for the Study of Host-Virus Interactions
Peng Fei Zou and Pin Nie
3. Northern Blot Detection of Virus-Derived Small Interfering RNAs in Caenorhabditis elegans Using Non-Radioactive Oligo Probes
Tianyun Long and Rui Lu
4. Extraction and qPCR-Based Detection of miRNAs from Cultured PBMCs of Bubaline Origin
Chandra S. Mukhopadhyay, Ramneek Verma, and Jasdeep Singh
5. Visualizing Virus-Derived dsRNA Using Antibody-Independent and -Dependent Methods
Sarah J. Poynter and Stephanie J. DeWitte-Orr
6. RNA PAMPs as Molecular Tools for Evaluating RIG-I Function in Innate Immunity
Renee C. Ireton, Courtney Wilkins, and Michael Gale, Jr.
7. Methods to Visualize MAVS Subcellular Localization
Christine Vazquez, Dia C. Beachboard, and Stacy M. Horner
8. Purification of Cyclic GMP-AMP from Viruses and Measurement of Its Activity in Cell Culture
Alice Mayer, Jonathan Maelfait, Anne Bridgeman, and Jan Rehwinkel
9. cGAMP Quantification in Virus-Infected Human Monocyte-Derived Cells by HPLC-Coupled Tandem Mass Spectrometry
Jennifer Paijo, Volkhard Kaever, and Ulrich Kalinke
10. Methods of Assessing STING Activation and Trafficking
Vladislav Pokatayev and Nan Yan
11. Genome-Wide CRISPR/Cas9 Screening for High-Throughput Functional Genomics in Human Cells
Shiyou Zhu, Yuexin Zhou, and Wensheng Wei
12. High Throughput Screening for Identification of Novel Innate Immune Activators
Bryan J. Gall and Victor R. DeFilippis
13. Chromosome Conformation Capture for Research on Innate Antiviral Immunity
Yoon Jung Kim and Tae Hoon Kim
14. Discovery of Variants Underlying Host Susceptibility to Virus Infection Using Whole-Exome Sequencing
Gabriel A. Leiva-Torres, Nestor Nebesio, and Silvia M. Vidal
15. Isolation, Purification, and Culture of Primary Murine Sensory Neurons
Sarah Katzenell, Jorge R. Cabrera, Brian J. North, and David A. Leib
16. Isolation of Group 2 Innate Lymphoid Cells from Mouse Lungs
Claudia U. Duerr and Joerg H. Fritz
17. Epidemiological Methods
Biao Wang and Mark Loeb
「Nielsen BookData」 より