Plant germline development : methods and protocols

著者

• Schmidt, Anja

書誌事項

Plant germline development : methods and protocols

edited by Anja Schmidt

（Methods in molecular biology, 1669）（Springer protocols）

Humana Press, c2017

注記

Includes bibliographical references and index

内容説明・目次

内容説明

This detailed volume explores common and numerous specialized methods to study various aspects of plant germline development and targeted manipulation, including imaging and hybridization techniques to study cell-type specification, cell lineage, signaling and hormones, cell cycle, and the cytoskeleton. In addition, cell-type specific methods for targeted ablation or isolation are provided, protocols to apply "omics" technologies and to perform bioinformatics data analysis, as well as methods relevant for aspects of biotechnology or plant breeding. This includes protocols that are relevant for the targeted manipulation of pathways, for crop plant transformation, or for conditional induction of phenotypes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Germline Development: Methods and Protocols serves as a comprehensive guide not only to studying basic questions related to different aspects of plant reproductive development but also for state of the art methods, in addition to being a source of inspiration for new approaches and research questions in many laboratories.

目次

Part I: Plant Germline Development: From Investigation to Application 1. An Introduction to Male Germline Development Hannes Vogler, Ueli Grossniklaus, and Afif Hedhly 2. Apomixis: Engineering the Ability to Harness Hybrid Vigor in Crop Plants Joann A. Conner and Peggy Ozias-Akins Part II: Clearing, Imaging Techniques, and Immunolocalization to Study Cell Lineage, Cell Type Specificity, Cytoskeleton, Cell Cycle, Signaling, and Hormones 3. 3D Imaging of Whole-Mount Ovules at Cellular Resolution to Study Female Germline Development in Rice Ethel Mendocilla Sato, Wenjing She, and Celia Baroux 4. Live-Cell Imaging of F-Actin Dynamics During Fertilization in Arabidopsis thaliana Daichi Susaki, Daisuke Maruyama, Ramesh Yelagandula, Frederic Berger, and Tomokazu Kawashima 5. Development and Observation of Mature Megagametophyte Cell-Specific Fluorescent Markers Mark A. Chamberlin and Shai J. Lawit 6. Analysis of Fluorescent Reporter Activity in the Male Germline During Pollen Development by Confocal Microscopy David Twell and Lynette Brownfield 7. In Vivo Ploidy Determination of Arabidopsis thaliana Male and Female Gametophytes Burcu Nur Keceli, Nico De Storme, and Danny Geelen 8. Staining and Clearing of Arabidopsis Reproductive Tissue for Imaging of Fluorescent Proteins Daniel Slane, Patrick Burgel, and Martin Bayer 9. Live Cell Imaging of Auxin and Cytokinin Signaling in Maize Female Gametophytes Antony M. Chettoor and Matthew M.S. Evans 10. Imaging Ca2+ Dynamics in Wild-Type and NADPH Oxidase-Deficient Mutant Pollen Tubes with Yellow Cameleon and Confocal Laser Scanning Microscopy Christina Maria Franck, Jens Westermann, and Aurelien Boisson-Dernier 11. Immunolocalization of AGPs and Pectins in Quercus suber Gametophytic Structures Mario Luis da Costa, Ana Lucia Lopes, Maria Isabel Amorim, and Silvia Coimbra Part III: Chromosome Spreading, Whole Mount In Situ, and Transmission Electron Microscopy (TEM) 12. Optimization of Cell Spreading and Image Quality for the Study of Chromosomes in Plant Tissues Laksana Kantama, Erik Wijnker, and Hans de Jong 13. Whole Mount RNA-FISH on Ovules and Developing Seeds Andrea Bleckmann and Thomas Dresselhaus 14. Analysis of Peroxisome Biogenesis in Pollen by Confocal Microscopy and Transmission Electron Microscopy Peng-Fei Jia, Hong-Ju Li, and Wei-Cai Yang 15. Transmission Electron Microscopy (TEM) to Study Histology of Pollen and Pollen Tubes Peng-Fei Jia, Hong-Ju Li, and Wei-Cai Yang Part IV: Isolation of Specific Cells of the Male and Female Germlines 16. Isolation of Arabidopsis Pollen, Sperm Cells, and Vegetative Nuclei by Fluorescence-Activated Cell Sorting (FACS) Mario R. Santos, Claudia Bispo, and Joerg D. Becker 17. Isolation of Rice Sperm Cells for Transcriptional Profiling Scott D. Russell, Daniel S. Jones, Sarah Anderson, Xinkun Wang, Venkatesan Sundaresan, and Xiaoping Gou 18. Manual Isolation of Living Cells from the Arabidopsis thaliana Female Gametophyte by Micromanipulation Maria Englhart, Lucija Soljic, and Stefanie Sprunck Part V: Omics Technologies and Bioinformatic Analyses 19. Isolation and Detection of Small RNAs from Pollen German Martinez 20. Analysis of Proteins Enriched in Rice Gamete Takashi Okamoto 21. Phosphoprotein Enrichment from Tobacco Mature Pollen Crude Protein Extract Jan Fila and David Honys 22. Identification of Cis-Regulatory Modules that Function in the Male Germline of Flowering Plants Benjamin Peters, Jack Aidley, Murray Cadzow, David Twell, and Lynette Brownfield 23. RNA-Seq Data Analysis: From Raw Data Quality Control to Differential Expression Analysis Weihong Qi, Ralph Schlapbach, and Hubert Rehrauer 24. RNA-Seq Data Analysis Protocol: Combining In-House and Publicly Available Data Marc W. Schmid Part VI: Technologies with Outreach for Plant Manipulation, Studies of Pathways, and Applications in Crop Species 25. Impedance Flow Cytometry as a Tool to Analyze Microspore and Pollen Quality Iris Heidmann and Marco Di Berardino < 26. Agrobacterium-Mediated Sorghum Transformation Emily Wu and Zuo-Yu Zhao 27. Use of the Cas9 Orthologues from Streptococcus thermophilus and Staphylococcus aureus for Non-Homologous End-Joining Mediated Site-Specific Mutagenesis in Arabidopsis thaliana Jeannette Steinert, Carla Schmidt, and Holger Puchta 28. Transgenic Reproductive Cell Ablation Shai J. Lawit and Mark A. Chamberlin 29. In Vivo Reporters for Protein Half-Life Pavel Reichman and Nico Dissmeyer 30. Conditional Modulation of Biological Processes by Low-Temperature Degrons Nico Dissmeyer

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詳細情報

• NII書誌ID(NCID): BB25616337
• ISBN:
  • 9781493972852
• LCCN: 2017950215
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xiii, 422 p.
• 大きさ: 27 cm
• 親書誌ID:
  • BA07889873
  • BA88873632