Affinity chromatography : methods and protocols
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Bibliographic Information
Affinity chromatography : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, v. 1286）（Springer protocols）
Humana Press, c2015
3rd ed
- : hbk
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
The aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols. Affinity Chromatography: Methods and Protocols, Third Edition guides readers through new state of the art protocols, molecular modelling, and the study of ligand-target interactions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Affinity Chromatography: Methods and Protocols, Third Edition is designed as a useful resource for those interested in the rapid and quantitative isolation of biomolecules with high purity.
Table of Contents
1. Affinity Chromatography: A Historical
David S. Hage and Ryan Matsuda
Part I: General Modes and Ligands of Affinity Chromatography
2. Protein Purification by Aminosquarylium Cyanine-Affinity Chromatography
Vania C. Graca, Fani Sousa, Paulo F. Santos and Paulo S. Almeida
3. One Step Purification of Phosphinothricin Acetyltransferase using Reactive Dye Affinity Chromatography
Cunxi Wang, Thomas C. Lee, Kathleen S. Crowley, and Erin Bell
4. Antibody Purification from Human Plasma by Metal-Chelated Affinity Membranes
Handan Yavuz, Nilay Bereli, Fatma Yilmaz, Canan Armutcu and Adil Denizli
5. Specific Recognition of Supercoiled Plasmid DNA by Affinity Chromatography using a Synthetic Aromatic Ligand
Catarina Caramelo-Nunes and Candida T. Tomaz
6. Cell Affinity Separations on Microfluidic Devices
Yan Gao, Wenjie Li, Ye Zhang and Dimitri Pappas
7. Aptamer-modified :Magnetic Beads in Affinity Separation of Proteins
Guohong Zhu and Johanna-Gabriela Walter
8. The Strep-tag System for One-step Purification and High Affinity Detection or Capturing of Proteins
Thomas Schmidt and Arne Skerra
9. Robotic High-throughput Purification of Affinity-tagged Recombinant Proteins
Simone C. Wiesler and Robert O. J. Weinzierl
Part II: Matrices in Affinity Chromatography
10. Macroporous Silica Particles Derivatized for Enhanced Lectin Affinity Enrichment of Glycoproteins
Ben Mann
11. Immobilized Magnetic Beads based Multi-Target Affinity Selection Coupled with HPLC-MS for Screening Active Compounds from Traditional Chinese Medicine and Natural Products
Yaqi Chen, Zhui Chen and Yi Wang
12. Mixed-bed Affinity Chromatography: Principles And Methods
Egisto Boschetti and Pier Giorgio Righetti
13. Preparation and characterization of fluorophenylboronic acid-functionalized Affinity Monolithic Columns for the Selective Enrichment of Cis-diol-containing Biomolecules
Qianjin Li and Zhen Liu
Part III: Macroporous Polymers as Matrix in Affinity Chromatography
14. Introduction to Macroporous Cryogels
Senta Reichelt
15. Cryogels with Affinity Ligands as Tools in Protein Purification
Solmaz Hajizadeh and Bo Mattiasson
16. Direct Capture of His6-tagged Proteins Using Megaporous Cryogels Developed for Metal-ion Affinity Chromatography
Naveen Kumar Singh, Roy N. DSouza, Noor Shad Bibi and Marcelo Fernandez-Lahore
17. Ni(II) Chelated IDA Functionalized Poly(HEMA-GMA) Cryogels for Urease Adsorption
Murat UYGUN
18. A Novel Chromatographic Media: Histidine Containing Composite Cryogels for HIgG Separation from Human Serum
Goezde Baydemir and Mehmet Odabasi
19. Molecularly Imprinted Cryogels for Human Serum Albumin Depletion
Muge Andac, Igor Yu Galaev, Handan Yavuz and Adil Denizli
20. Interpenetrating Polymer Network Composite Cryogels with Tailored Porous Morphology and Sorption Properties
Ecaterina Stela Dragan and Maria Valentina Dinu
Part IV: Spectroscopy, Binding Studies and Molecular Modelling
21. Analysis of Drug-Protein Interactions by High-Performance Affinity Chromatography: Interactions of Sulfonylurea Drugs with Normal and Glycated Human Serum Albumin
Ryan Matsuda, Jeanethe Anguizola, Krina S. Hoy and David S. Hage
22. Accurate protein-peptide Titration Experiments by Nuclear Magnetic Resonance using Low-volume Samples
Christian Koehler, Raphael Recht, Marc Quinternet, Frederic de Lamotte, Marc-Andre Delsuc, and Bruno Kieffer
23. Characterization of the Binding Strengths between Boronic Acids and Cis-Diol-containing Biomolecules by Affinity Capillary Electrophoresis
Chenchen Lu and Zhen Liu
24. Determination of the Kinetic Rate Constant of Cyclodextrin Supramolecular Systems by High Performance Affinity Chromatography
Jiwen Zhang, Haiyan Li, Lixin Sun, and Caifen Wang
25. Molecular Modeling of the Affinity Chromatography of Monoclonal Antibodies
Matteo Paloni and Carlo Cavallotti
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