Molecular toxicology protocols
著者
書誌事項
Molecular toxicology protocols
（Methods in molecular biology / John M. Walker, series editor, 1105）（Springer protocols）
Humana Press, c2014
2nd ed
- : hbk
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Molecular Toxicology Protocols, Second Edition aims to bring together a series of articles describing validated methods to elucidate specific molecular aspects of toxicology, the emphasis being on the application of molecular methods to genetic toxicology. The volume is divided into ten parts, roughly corresponding to the spectrum of biomarkers intermediate between exposure and disease outcomes as proposed in molecular epidemiology models. Subjects of these new chapters range from preparation of fluid specimens for analysis of cellular inflammatory responses to genotoxic insults to sensitive methods for proteomic analysis and aberrant DNA methylation patterns. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Molecular Toxicology Protocols, Second Edition addresses not only the needs of molecular biologists and toxicologists, but also those of individuals interested in applying molecular methods to clinical applications, such as geneticists, pathologists, biochemists, and epidemiologists.
目次
- Part I. Toxicoproteomics 1. Array-Based Immunoassays with Rolling-Circle Amplification Detection Katie Partyka, ShuangshuangWang, Ping Zhao, Brian Cao, and Brian Haab 2. Analysis of Protein Changes Using Two Dimensional Difference Gel Electrophoresis Weimin Gao Part II. Toxicant-Induced Inflammation 3. Assessment of Pathological and Physiological Changes in Mouse Lung Through Bronchoalveolar Lavage Yuanpu Peter Di 4. Analysis of Clinical and Biological Samples Using Microsphere-Based Multiplexing Luminex System Yingze Zhang, Rahel Birru, and Y. Peter Di Part III. Gene Promoter Methylation 5. Detection of DNA Methylation by MeDIP and MBDCap Assays: An Overview of Techniques Hang-Kai Hsu, Yu-I Weng, Pei-Yin Hsu, Tim H.-M. Huang, and Yi-Wen Huang 6. Screening of DNA Methylation Changes by Methylation-Sensitive Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR) Kamaleshwar P. Singh Part IV. Array Technologies 7. Strategies for Measurement of Biotransformation Enzyme Gene Expression Marjorie Romkes and Shama C. Buch 8. Genotyping Technologies: Application to Biotransformation Enzyme Genetic Polymorphism Screening Marjorie Romkes and Shama C. Buch 9. TaqMan Fluorogenic Detection System to Analyze Gene Transcription in Autopsy Material Kaori Shintani-Ishida, Bao-Li Zhu, and Hitoshi Maeda Part V. Analysis of DNA Adducts 10. 32P-Postlabelling Analysis of DNA Adducts David H. Phillips and Volker M. Arlt 11. Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot Masako Ochiai, Takashi Sugimura, and Minako Nagao 12. DNA Isolation and Sample Preparation for Quantitation of Adduct Levels by Accelerator Mass Spectrometry Karen H. Dingley, Esther A. Ubick, John S. Vogel, Ted J. Ognibene, Michael A. Malfatti, Kristen Kulp, and Kurt W. Haack 13. Analysis of DNA Strand Cleavage at Abasic Sites Walter A. Deutsch and Vijay Hegde Part VI. Detection of Chromosomal and Genome-Wide Damage 14. Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes without Mitogen Stimulation for Chromosome Aberration Analysis Using Specific Whole Chromosome DNA Hybridization Probes Rupak Pathak and Pataje G. S. Prasanna 15. Mutagen Sensitivity as Measured by Induced Chromatid Breakage as a Marker of Cancer Risk Xifeng Wu, Yun-Ling Zheng, and T. C. Hsu 16. Pulsed-Field Gel Electrophoresis Analysis of Multicellular DNA Double-Strand Break Damage and Repair Nina Joshi and Stephen G. Grant Part VII. Detection and Characterization of Surrogate Gene Mutation 17. Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and Mice Vasily N. Dobrovolsky, Xuefei Cao, Javed A. Bhalli, and Robert H. Heflich 18. The Blood-Vased Glycophorin A Human In vivo Somatic Mutation Assay Nicole T. Myers and Stephen G. Grant 19. Flow Cytometric Quantification of Mutant T Cells with Altered Expression of the T-Cell Receptor: Detecting Somatic Mutants in Humans and Mice Seishi Kyoizumi, Yoichiro Kasunoki, and Tomonori Hayashi 20. Analysis of in Vivo Mutation at the HPRT and TK Genes of Mouse Lymphocytes Vasily N. Dobrovolsky, Joseph G. Shaddock, and Robert H. Heflich 21. Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems Roy R. Swiger 22. The Human T-Cell Cloning Assay: Identifying Genotypes Susceptible to Drug Toxicity and Somatic Mutation Sai-Mei Hou 23. Molecular Analysis of HPRT Mutations in Human Lymphocytes Phouthone Keohavong, Liqiang Xi, Stephen G. and Grant 24. Simultaneous Quantification of t(14
- 18) and HPRT Exon 2/3 Deletions in Human Lymphocytes James C. Fuscoe Part VIII. Detection and Characterization of Cancer Gene Mutation 25. Mutation Screening of the TP53 Gene by Temporal Temperature Gel Electrophoresis Therese Sorlie, Hilde Johnson, Phuong Vu, Guro Elisabeth Lind, Ragnhild Lothe, and Anne-Lise Borreson-Dale 26. Detection of Point Mutations of K-ras Oncogene and p53 Tumor Suppressor Gene in Sputum Samples Wei-Min Gao and Phouthone Keohavong 27. ACB-PCR Quantification of Somatic Oncomutations Meagan B. Myers, Page B. McKinzie, Yiying Wang, Fanxue Meng, and Barbara L. Parsons 28. Gel-Based Nonradioactive Single Strand Conformational Polymorphism and Mutation Detection Vibhuti Gupta, Reetakshi Arora, Sailesh Gochhait, Narendra K. Bairwa, and Rameshwar N. K. Bamezai 29. Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesion Toshinari Minamoto 30. Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR Sheetal Sing, Shyh-Jen Shih, and Andrew T. M. Vaughan Part IX. Analysis of DNA Damage and Repair Mechanisms 31. Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian Cells Amy Furda, Janine H. Santos, Joel N. Meyer, and Bennett Van Houten 32. The Sister Chromatid Exchange (SCE) Assay Dawn M. Stults, Michael W. Killen, and Andrew J. Pierce 33. The Gene Cluster Instability (GCI) Assay for Recombination Michael W. Killen, Dawn M. Stults, and Andrew J. Pierce 34. Measuring Recombination Proficiency in Mouse Embryonic Stem Cells Andrew J. Pierce and Maria Jasin 35. Microsatellite Instability: An Indirect Assay to Detect Defects in the Cellular Mismatch Repair Machinery Narendra K. Bairwa, Anjana Saha, Sailesh Gochhait, Ranjana Pal, Vibhuti Gupta, and Rameshwar N. K. Bamezai 36. Unscheduled DNA Synthesis: The Clinical and Functional Assay for Global Genomic Nucleotide Excision Repair Jean J. Latimer, and Carytal M. Kelly 37. An Immunoassay for Measuring Repair of UV Photoproducts Shirley McCready 38. Analysis of Double-Strand Break Repair by Non-Homologous DNA End Joining in Cell-Free Extracts from Mammalian Cells Petra Pfeiffer, Andrea Odersky, Wolfgang Goedecke, and Steffi Kuhfittig-Kulle Part X. Analysis of Cellular Bioenergetics and Apoptosis 39. Bioenergetic Analysis of Intact Mammalian Cells Using the Seahorse XF42 Extracellular Flux Analyzer and a Luciferase ATP Assay Michelle Barbi de Moura, and Bennett Van Houten 40. Quantification of Selective Phosphatidylserine Oxidation During Apoptosis James P. Fabisiak, Yulia Y. Tyurina, and Valerian E. Kagan 41. Quantitative Method of Measuring Phophatidylserine Externalization During Apoptosis Using Electron Paramagnetic Resonance (EPR) Spectroscopy and Annexin-Conjugated Iron James P. Fabisiak, Grigory G. Borisenko, and Valerian E. Kagan 42. Detection of Programmed Cell Death in Cells Exposed to Genotoxic Agents Using a Caspase Activation Assay Madhu Gupta, Madhumita Santra, and Patrick P. Koty
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