Metabonomics : methods and protocols
著者
書誌事項
Metabonomics : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, 1265)
Humana Press, c2015
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注記
"Softcover reprint of the hardcover 1st edition 2015"--T.p. verso
Includes bibliographical references and index
内容説明・目次
内容説明
This volume on metabonomics provides detailed information on the procedures involved in nuclear magnetic resonance (NMR) spectroscopy, gas chromatography-mass spectrometry (GS-MS), liquid chromatography-mass spectrometry (LC-MS), and capillary electrophoresis-mass spectrometry (CE-MS). Chapters focus on technologies and chemometrics, generation of metabonomics data, extraction of meaningful information from data, drug development, toxicology, diagnostics, and describing metabonomics as an essential part of systems biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
目次
1. Metabonomics - Analytical Techniques and Associated Chemometrics at a Glance
Jacob T. Bjerrum
2. Sample Collection and Preparation of Biofluids and Extracts for NMR Spectroscopy
Gwenaelle Le Gall
3. NMR Spectroscopy of Biofluids and Extracts
Gwenaelle Le Gall
4. High-resolution Magic-Angle-Spinning NMR Spectroscopy of Intact Tissue
Guro F. Giskeodegard, Maria D. Cao, and Tone F. Bathen
5. Sample Collection and Preparation of Biofluids and Extracts For Liquid Chromatography-Mass Spectrometry
Peiyuan Yin, Lina Zhou, Xinjie Zhao, and Guowang Xu
6. Liquid Chromatography-Mass Spectrometry of Biofluids and Extracts
Xinjie Zhao, Lina Zhou, Peiyuan Yin, and Guowang Xu
7. Sample Collection and Preparation of Biofluids and Extracts For Gas-Chromatography-Mass Spectrometry
Abdul-Hamid M. Emwas, Zeyad A. Al-Talla, and Najeh M. Kharbatia
8. Gas Chromatography-Mass Spectrometry of Biofluids and ExtractsAbdul-Hamid M. Emwas, Zeyad A. Al-Talla, Yang Yang, and Najeh M. Kharbatia
9. Capillary Electrophoresis Mass SpectrometryMasataka Wakayama, Akiyoshi Hirayama, and Tomoyoshi Soga
10. Preprocessing of Raw Metabonomics Data
Riyas Vettukattil
11. Extracting Meaningful Information From Metabonomics Data Using Multivariate Statistics
Max Bylesjoe
12. Combining Metabonomics and Other -omics DataMattias Rantalainen
13. The Strengths and Weaknesses of NMR Spectroscopy and Mass Spectrometry With Particular Focus on Metabolomics Research
Abdul-Hamid M. Emwas
14. Metabonomics and Drug Development
Pranov Ramana, Erwin Adams, Patrick Augustijns, and Ann Van Schepdael
15. Metabonomics and Toxicology
Liang Zhao and Thomas Hartung
16. Metabonomics and Diagnostics
Lucy C. Hicks, Simon J.L. Ralphs, and Horace R.T. Williams
17. Metabonomics and Systems Biology
Vicky De Preter
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