CiNii Books - Extracellular vesicles : methods and protocols

This volume examines established methods and protocols to isolate and characterize extracellular vesicles (EVs) and their composition, among other techniques including purification, imaging, biofluid-specific and cell-specific isolation and downstream genomic and proteomic profiling. The international group of expert scientists who have contributed to this collection provide a variety of different techniques related to the growing assortment of EV applications, given that at times using only one technique or two is insufficient to address the question at hand. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Extracellular Vesicles: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of EV functions and applications.

Table of Contents

1. Extracellular Vesicles: A Brief Overview and Its Role in Precision Medicine Mingyu Shang, John Ji, Chao Song, Bao Jun Gao, Jason Gang Jin, Winston Patrick Kuo, and Hongjun Kang 2. Red Blood Cells: A Source of Extracellular Vesicles Winston Patrick Kuo, John Tigges, Vasilis Toxavidis, and Ionita Ghiran Part I: Isolation of Extracellular Vesicles 3. Isolation of Extracelullar Vesicles by Ultracentrifugation Fatemeh Momen-Heravi 4. Sequential Filtration: A Gentle Method for the Isolation of Functional Extracellular Vesicles Mitja L. Heinemann and Jody Vykoukal 5. Paper-Based Devices for Isolation of Extracellular Vesicles Yi-Hsing Hsiao and Chihchen Chen 6. Filter-Based Extracellular Vesicle mRNA Isolation and High-Throughput Gene Expression Analysis Cindy M. Yamamoto, Taku Murakami, and Shu-Wing Ng 7. Specific and Generic Isolation of Extracellular Vesicles with Magnetic Beads Ketil W. Pedersen, Bente Kierulf, and Axl Neurauter Part II: Purification of Extracellular Vesicles 8. Polymer-Based Purification of Extracellular Vesicles Peter N. Brown and Hang Yin 9. Size Exclusion Chromatography: A Simple and Reliable Method for Exosome Purification Richard Lobb and Andreas Moeller 10. Purification Protocols for Extracellular Vesicles Rebecca E. Lane, Darren Korbie, Matt Trau, and Michelle M. Hill Part III: Characterization of Extracellular Vesicles 11. Characterization of Extracellular Vesicles by Surface Plasmon Resonance Hyungsoon Im, Katherine Yang, Hakho Lee, and Cesar M. Castro 12. Extracellular Vesicle Isolation and Analysis by Western Blotting Emma J.K. Kowal, Dmitry Ter-Ovanesyan, Aviv Regev, and George M. Church 13. Analysis of Extracellular Vesicles Using Fluorescence Nanoparticle Tracking Analysis Pauline Carnell-Morris, Dionne Tannetta, Agnieszka Siupa, Patrick Hole, and Rebecca Dragovic 14. Characterization of Extracellular Vesicles by Flow Cytometry Virginia Camacho, Vasilis Toxavidis, and John C. Tigges 15. Characterization of Extracellular Vesicles by Size-Exclusion High-Performance Liquid Chromatography (HPLC) Tao Huang and Jiang He 16. Multi-Surface Antigen Staining of Larger Extracellular Vesicles Veronika Lukacs-Kornek, Henrike Julich, Sabine Katharina Urban, and Miroslaw Kornek 17. Microcapillary Chip-Based Extracellular Vesicle Profiling System Takanori Akagi and Takanori Ichiki Part IV: Imaging of Extracellular Vesicles 18. Detection and Characterization of Extracellular Vesicles by Transmission and Cryo-Transmission Electron Microscopy Petr Cizmar and Yuana Yuana 19. Imaging of Isolated Extracellular Vesicles Using Fluorescence Microscopy Dmitry Ter-Ovanesyan, Emma J.K. Kowal, Aviv Regev, George M. Church, and Emanuele Cocucci Part V: Tracking of Extracellular Vesicles 20. In Vivo Tracking of Extracellular Vesicles in Mice Using Fusion Protein Comprising Lactadherin and Gaussia Luciferase Yuki Takahashi, Makiya Nishikawa, and Yoshinobu Takakura 21. Tracking Extracellular Vesicles Delivery and RNA Translation Using Multiplexed Reporters Anthony Yan-Tang Wu and Charles Pin-Kuang Lai Part VI: Downstream Extracellular Vesicle Applications Genomics and Proteomics 22. Extraction and Analysis of Extracellular Vesicle-Associated miRNAs Following Antibody-Based Extracellular Vesicle Capture from Plasma Samples Davide Zocco and Natasa Zarovni 23. Extracellular Vesicle miRNA Detection Using Molecular Beacons Won Jong Rhee and Seunga Jeong 24. Rapid Isolation of Extracellular Vesicles from Blood Plasma with Size-Exclusion Chromatography Followed by Mass Spectrometry-Based Proteomic Profiling Simion Kreimer and Alexander R. Ivanov 25. An Adaptable Polyethylene Glycol-Based Workflow for Proteomic Analysis of Extracellar Vesicles Stephanie N. Hurwitz and David G. Meckes, Jr. Part VII: Isolation of Extracellular Vesicles from Biofluids 26. Isolation of Extracellular Vesicles from Ovarian Follicular Fluid: Evaluation of Ultracentrifugation and Commercial Kits Shlomit Kenigsberg, Clifford C. Librach, and Juliano C. da Silveira 27. Isolation of Extracellular Vesicles in Saliva Using Density Gradient Ultracentrifugation Kazuya Iwai, Satoshi Yamamoto, Mitsutaka Yoshida, and Kiyotaka Shiba 28. Isolation of Extracellular Vesicles from Breast Milk Xin Wang 29. An Integrated Double-Filtration Microfluidic Device for Detection of Extracellular Vesicles from Urine for Bladder Cancer Diagnosis Li-Guo Liang, Ye-Feng Sheng, Sherry Zhou, Fatih Inci, Lanjuan Li, Utkan Demirci, and ShuQi Wang Part VIII: Extracellular Vesicles Isolated from Cell Culture, Parasites, and Stem Cells 30. Electric Field-Induced Disruption and Releasing Viable Content from Extracellular Vesicles Chris Wang, Austin Wang, Fang Wei, David T.W. Wong, and Michael Tu 31. Production and Characterization of Extracellular Vesicles in Malaria Smart Mbagwu, Michael Walch, Luis Filgueira, and Pierre-Yves Mantel 32. Isolation of Extracellular Vesicles from Stem Cells Zixin Chen, Yongjun Li, Hong Yu, Yan Shen, Chengwei Ju, Genshan Ma, Yutao Liu, Il-man Kim, Neal L. Weintraub, and Yaoliang Tang Part IX: Extracellular Vesicles in Mouse Models 33. The Use of Peripheral Extracellular Vesicles for Identification of Molecular Biomarkers in a Solid Tumor Mouse Model Noemi Garcia-Romero, Gorjana Rackov, Cristobal Belda-Iniesta, and Angel Ayuso-Sacido Part X: Therapeutic Applications of Extracellular Vesicles 34. Therapeutic Applications of Extracellular Vesicles: Perspectives from Newborn Medicine Gareth R. Willis, Stella Kourembanas, and S. Alex Mitsialis 35. Therapeutic Use of Tumor Cell-Derived Extracellular Vesicles Jing Liu, Jingwei Ma, Ke Tang, and Bo Huang

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