Cancer stem cells : methods and protocols
著者
書誌事項
Cancer stem cells : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1692）（Springer protocols）
Humana Press, c2018
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This detailed volume gathers a comprehensive collection of methods, protocols, and procedures used for the identification, characterization, and selection of cancer stem cells. These include surface marker expression, side population, spheres formation, ALDH activity, and numerous others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert tips and troubleshooting advice to avoid potential pitfalls.
Comprehensive and practical, Cancer Stem Cells: Methods and Protocols provides researchers with the techniques they will need in order to pursue vital research into this complex field of study.
目次
1. Introduction to Cancer Stem Cells: Past, Present, and Future
David Bakhshinyan, Ashley A. Adile, Maleeha A. Qazi, Mohini Singh, Michelle M. Kameda-Smith, Nick Yelle, Chirayu Chokshi, Chitra Venugopal, and Shiela K. Singh
2. Surface Markers for the Identification of Cancer Stem Cells
Vinod Gopalan, Farhadul Islam, and Alfred King-yin Lam
3. The Role of CD44 and Cancer Stem Cells
Liang Wang, Xiangsheng Zuo, Keping Xie, and Daoyan Wei
4. Evaluation and Isolation of Cancer Stem Cells Using ALDH Activity Assay
Luigi Mele, Davide Liccardo, and Virginia Tirino
5. Isolation of Cancer Stem Cells by Side Population Method
Masayuki Shimoda, Masahide Ota, and Yasunori Okada
6. Self-Renewal and CSCs In Vitro Enrichment: Growth as Floating Spheres
Pooja Mehta, Caymen Novak, Shreya Raghavan, Maria Ward, and Geeta Mehta
7. In Vitro Tumorigenic Assay: The Tumor Spheres Assay
Hui Wang, Anna Paczulla, Martina Konantz, and Claudia Lengerke
8. In Vitro Tumorigenic Assay: Colony Forming Assay for Cancer Stem Cells
Vijayalakshmi Rajendran and Mayur Vilas Jain
9. Xenograft as In Vivo Experimental Model
Manuela Porru, Luca Pompili, Carla Caruso, and Carlo Leonetti
10. How to Assess Drug Resistance in Cancer Stem Cells
Maria Laura De Angelis, Ruggero De Maria, and Marta Baiocchi
11. Tumor Tissue Analogs for Assessment of Radioresistance in Cancer Stem Cells
Meenakshi Upreti
12. Generation of In Vitro Model of Epithelial Mesenchymal Transition (EMT) Via the Expression of a Cytoplasmic Mutant Form of Promylocytic Leukemia Protein (PML)
Anna Di Biase, Amanda K. Miles, and Tarik Regad
13. Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System
Magdalena E. Buczek, Stephen P. Reeder, and Tarik Regad
14. Determination of miRNAs from Cancer Stem Cells Using a Low Density Array Platform
Hiromichi Kawasaki, Angela Lombardi, and Michele Caraglia
15. Assessing DNA Methylation in Cancer Stem Cells
Sudipto Das, Bruce Moran, and Antoinette S. Perry
16. Histones Acetylation and Cancer Stem Cells (CSCs)
Vivian Petersen Wagner, Manoela Domingues Martins, and Rogerio Moraes Castilho
17. Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods
Martina Intartaglia, Rosalaura Sabetta, Monica Gargiulo, Giovanna Roncador, Federica Zito Marino, and Renato Franco
18. Circulating Tumor Cells
Sebastian A. Garcia, Jurgen Weitz, and Sebastian Schoelch
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